Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce
Selenium (Se) uptake by plants depends on its form and salicylic acid (SA) can increase the efficiency of plant biofortification in Se. This study investigated the effects of selenite (Na<sub>2</sub>SeO<sub>3</sub>) and selenomethionine (SeMet) applied individually or togethe...
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MDPI AG
2020-11-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/10/12/584 |
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| author | Iwona Kowalska Sylwester Smoleń Małgorzata Czernicka Maryia Halka Kinga Kęska Joanna Pitala |
| author_facet | Iwona Kowalska Sylwester Smoleń Małgorzata Czernicka Maryia Halka Kinga Kęska Joanna Pitala |
| author_sort | Iwona Kowalska |
| collection | DOAJ |
| description | Selenium (Se) uptake by plants depends on its form and salicylic acid (SA) can increase the efficiency of plant biofortification in Se. This study investigated the effects of selenite (Na<sub>2</sub>SeO<sub>3</sub>) and selenomethionine (SeMet) applied individually or together with SA on a total content of Se, Se speciation forms, yield and content of sugars and ascorbic acid of lettuce, as well as activity of selenocysteine methyltransferase (<i>smt</i>) and methionine methyltransferase (<i>mmt</i>) genes of the Se metabolic pathway. Lettuce was grown in the nutrient film technique (NFT) system. Se and SA were used at doses of 0.5 and 10.0 mg dm<sup>−3</sup> of the nutrient solution, respectively. The treatments were: 1. control, 2. Na<sub>2</sub>SeO<sub>3</sub>, 3. Na<sub>2</sub>SeO<sub>3</sub> + SA, 4. SeMet, 5. SeMet + SA, 6. SA. Se was accumulated more in the roots than the leaves. SeMet was more effective in biofortification than Na<sub>2</sub>SeO<sub>3</sub>. SA enhanced Se uptake, especially organic Se. Plants supplied with SeMet alone or SeMet + SA accumulated in their leaves mainly SeMet and methylselenocysteine (MeSeCys), while those treated with Na<sub>2</sub>SeO<sub>3</sub> or Na<sub>2</sub>SeO<sub>3</sub> + SA accumulated MeSeCys and selenite (SeO<sub>3</sub><sup>−2</sup>). The roots showed no expression of <i>smt</i>, while the expression of two <i>mmt</i> genes was independent of either Se or SA. The leaves of plants supplied with Na<sub>2</sub>SeO<sub>3</sub> demonstrated the strongest expression of <i>mmt</i> and <i>smt</i>. |
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| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T14:32:22Z |
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| spelling | doaj.art-bcd6ca63388f4415bf4e4527786365be2023-11-20T22:28:56ZengMDPI AGAgriculture2077-04722020-11-01101258410.3390/agriculture10120584Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown LettuceIwona Kowalska0Sylwester Smoleń1Małgorzata Czernicka2Maryia Halka3Kinga Kęska4Joanna Pitala5Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandDepartment of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandLaboratory of Mass Spectrometry, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, PolandSelenium (Se) uptake by plants depends on its form and salicylic acid (SA) can increase the efficiency of plant biofortification in Se. This study investigated the effects of selenite (Na<sub>2</sub>SeO<sub>3</sub>) and selenomethionine (SeMet) applied individually or together with SA on a total content of Se, Se speciation forms, yield and content of sugars and ascorbic acid of lettuce, as well as activity of selenocysteine methyltransferase (<i>smt</i>) and methionine methyltransferase (<i>mmt</i>) genes of the Se metabolic pathway. Lettuce was grown in the nutrient film technique (NFT) system. Se and SA were used at doses of 0.5 and 10.0 mg dm<sup>−3</sup> of the nutrient solution, respectively. The treatments were: 1. control, 2. Na<sub>2</sub>SeO<sub>3</sub>, 3. Na<sub>2</sub>SeO<sub>3</sub> + SA, 4. SeMet, 5. SeMet + SA, 6. SA. Se was accumulated more in the roots than the leaves. SeMet was more effective in biofortification than Na<sub>2</sub>SeO<sub>3</sub>. SA enhanced Se uptake, especially organic Se. Plants supplied with SeMet alone or SeMet + SA accumulated in their leaves mainly SeMet and methylselenocysteine (MeSeCys), while those treated with Na<sub>2</sub>SeO<sub>3</sub> or Na<sub>2</sub>SeO<sub>3</sub> + SA accumulated MeSeCys and selenite (SeO<sub>3</sub><sup>−2</sup>). The roots showed no expression of <i>smt</i>, while the expression of two <i>mmt</i> genes was independent of either Se or SA. The leaves of plants supplied with Na<sub>2</sub>SeO<sub>3</sub> demonstrated the strongest expression of <i>mmt</i> and <i>smt</i>.https://www.mdpi.com/2077-0472/10/12/584seleniteorganic seleniumbiofortification<i>smt</i><i>mmt</i> |
| spellingShingle | Iwona Kowalska Sylwester Smoleń Małgorzata Czernicka Maryia Halka Kinga Kęska Joanna Pitala Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce Agriculture selenite organic selenium biofortification <i>smt</i> <i>mmt</i> |
| title | Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce |
| title_full | Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce |
| title_fullStr | Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce |
| title_full_unstemmed | Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce |
| title_short | Effect of Selenium Form and Salicylic Acid on the Accumulation of Selenium Speciation Forms in Hydroponically Grown Lettuce |
| title_sort | effect of selenium form and salicylic acid on the accumulation of selenium speciation forms in hydroponically grown lettuce |
| topic | selenite organic selenium biofortification <i>smt</i> <i>mmt</i> |
| url | https://www.mdpi.com/2077-0472/10/12/584 |
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