Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization
Recently, the demands of biofuels have increased, because of their significant role in reducing various pollutants created by fossil fuels. Here, we have collected 25 samples containing various thermotolerant microorganisms from the nine natural fermented sources of Bangladesh, such as Boiled potato...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-11-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1228477 |
| _version_ | 1818826070906896384 |
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| author | Ali Azam Talukder Farhana Easmin Siraje Arif Mahmud Mamoru Yamada |
| author_facet | Ali Azam Talukder Farhana Easmin Siraje Arif Mahmud Mamoru Yamada |
| author_sort | Ali Azam Talukder |
| collection | DOAJ |
| description | Recently, the demands of biofuels have increased, because of their significant role in reducing various pollutants created by fossil fuels. Here, we have collected 25 samples containing various thermotolerant microorganisms from the nine natural fermented sources of Bangladesh, such as Boiled potato (Bp), Decomposed foods (Df), Municipal liquid waste (Mlw), Municipal solid waste (Msw), Sugarcane juice (Sc), Pantavat (Pv), Sugar molasses (Sm), Tari (Tari) and Watermelon juice (Wm) for bioethanol production. Among them, 18 isolates are capable of producing bioethanol. Cultural, morphological, physiological, biochemical and genetic analyses were carried out under various physiological conditions. Ethanol fermentation was checked by different carbon sources, temperatures and pH. All of the isolates could grow well in the medium containing Dextrose and Arabinose and only two strains Pv-1 and Bp-2 could ferment Xylose as a sole carbon source. At 42 °C, the highest ethanol concentration 6.58% (v/v) was obtained by a strain Wm-1 isolated from Watermelon juice. At 37 °C, maximal ethanol concentrations of 6.74% (v/v), 6.50% (v/v) and 6.22% (v/v) were obtained by the strains Bp-2, Wm-l and Pv-1, respectively. Among the various pH tested, the highest ethanol concentration 6.6% (v/v) was obtained at pH 4.5 by a strain named Tari-2. Finally, yeast 26S rDNA sequencing information identified the strains Sc-2 as Saccharomyces cerevisiae Pv-2, Tari-2 and Df-1 as Pichia kudriavzevii, Mlw-l and Bp-2 as Candida tropicalis, Pv-1 as Pichia guilliermondii and Df-2 as Candida rugosa. |
| first_indexed | 2024-12-19T00:21:49Z |
| format | Article |
| id | doaj.art-bcd8ac7bad0c4fa9ad3bfd7777df5abc |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-19T00:21:49Z |
| publishDate | 2016-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-bcd8ac7bad0c4fa9ad3bfd7777df5abc2022-12-21T20:45:31ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302016-11-013061106111410.1080/13102818.2016.12284771228477Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterizationAli Azam Talukder0Farhana Easmin1Siraje Arif Mahmud2Mamoru Yamada3Jahangirnagar UniversityJahangirnagar UniversityJahangirnagar UniversityYamaguchi UniversityRecently, the demands of biofuels have increased, because of their significant role in reducing various pollutants created by fossil fuels. Here, we have collected 25 samples containing various thermotolerant microorganisms from the nine natural fermented sources of Bangladesh, such as Boiled potato (Bp), Decomposed foods (Df), Municipal liquid waste (Mlw), Municipal solid waste (Msw), Sugarcane juice (Sc), Pantavat (Pv), Sugar molasses (Sm), Tari (Tari) and Watermelon juice (Wm) for bioethanol production. Among them, 18 isolates are capable of producing bioethanol. Cultural, morphological, physiological, biochemical and genetic analyses were carried out under various physiological conditions. Ethanol fermentation was checked by different carbon sources, temperatures and pH. All of the isolates could grow well in the medium containing Dextrose and Arabinose and only two strains Pv-1 and Bp-2 could ferment Xylose as a sole carbon source. At 42 °C, the highest ethanol concentration 6.58% (v/v) was obtained by a strain Wm-1 isolated from Watermelon juice. At 37 °C, maximal ethanol concentrations of 6.74% (v/v), 6.50% (v/v) and 6.22% (v/v) were obtained by the strains Bp-2, Wm-l and Pv-1, respectively. Among the various pH tested, the highest ethanol concentration 6.6% (v/v) was obtained at pH 4.5 by a strain named Tari-2. Finally, yeast 26S rDNA sequencing information identified the strains Sc-2 as Saccharomyces cerevisiae Pv-2, Tari-2 and Df-1 as Pichia kudriavzevii, Mlw-l and Bp-2 as Candida tropicalis, Pv-1 as Pichia guilliermondii and Df-2 as Candida rugosa.http://dx.doi.org/10.1080/13102818.2016.1228477BioethanolDecomposed foodPantavatTarithermotolerant microorganisms |
| spellingShingle | Ali Azam Talukder Farhana Easmin Siraje Arif Mahmud Mamoru Yamada Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization Biotechnology & Biotechnological Equipment Bioethanol Decomposed food Pantavat Tari thermotolerant microorganisms |
| title | Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization |
| title_full | Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization |
| title_fullStr | Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization |
| title_full_unstemmed | Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization |
| title_short | Thermotolerant yeasts capable of producing bioethanol: isolation from natural fermented sources, identification and characterization |
| title_sort | thermotolerant yeasts capable of producing bioethanol isolation from natural fermented sources identification and characterization |
| topic | Bioethanol Decomposed food Pantavat Tari thermotolerant microorganisms |
| url | http://dx.doi.org/10.1080/13102818.2016.1228477 |
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