Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs.
In many parts of the world the damaging potato late blight pathogen, Phytophthora infestans, is spread as a succession of clonal lineages. The discrimination of genetic diversity within such evolving populations provides insights into the processes generating novel lineages and the pathways and driv...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0208606 |
| _version_ | 1819016547657580544 |
|---|---|
| author | Frank N Martin Yonghong Zhang David E L Cooke Mike D Coffey Niklaus J Grünwald William E Fry |
| author_facet | Frank N Martin Yonghong Zhang David E L Cooke Mike D Coffey Niklaus J Grünwald William E Fry |
| author_sort | Frank N Martin |
| collection | DOAJ |
| description | In many parts of the world the damaging potato late blight pathogen, Phytophthora infestans, is spread as a succession of clonal lineages. The discrimination of genetic diversity within such evolving populations provides insights into the processes generating novel lineages and the pathways and drivers of pathogen evolution and dissemination at local and global scales. This knowledge, in turn, helps optimise management practices. Here we combine two key methods for dissecting mitochondrial and nuclear diversity and resolve intra and inter-lineage diversity of over 100 P. infestans isolates representative of key clonal lineages found globally. A novel set of PCR primers that amplify five target regions are provided for mitochondrial DNA sequence analysis. These five loci increased the number of mtDNA haplotypes resolved from four with the PCR RFLP method to 37 (17, 6, 8 and 4 for Ia, Ib, IIa, and IIb haplotypes, respectively, plus 2 Herb-1 haplotypes). As with the PCR RFLP method, two main lineages, I and II were defined. Group I contained 25 mtDNA haplotypes that grouped broadly according to the Ia and Ib types and resolved several sub-clades amongst the global sample. Group II comprised two distinct clusters with four haplotypes corresponding to the RFLP type IIb and eight haplotypes resolved within type IIa. The 12-plex SSR assay revealed 90 multilocus genotypes providing accurate discrimination of dominant clonal lineages and other genetically diverse isolates. Some association of genetic diversity and geographic region of contemporary isolates was observed; US and Mexican isolates formed a loose grouping, distinct from isolates from Europe, South America and other regions. Diversity within clonal lineages was observed that varied according to the age of the clone. In combination, these fine-scale nuclear and maternally inherited mitochondrial markers enabled a greater level of discrimination among isolates than previously available and provided complementary perspectives on evolutionary questions relating to the diversity, phylogeography and the origins and spread of clonal lineages of P. infestans. |
| first_indexed | 2024-12-21T02:49:22Z |
| format | Article |
| id | doaj.art-bcd9e8159f3e40a9ad1e1760691442ca |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T02:49:22Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bcd9e8159f3e40a9ad1e1760691442ca2022-12-21T19:18:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01141e020860610.1371/journal.pone.0208606Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs.Frank N MartinYonghong ZhangDavid E L CookeMike D CoffeyNiklaus J GrünwaldWilliam E FryIn many parts of the world the damaging potato late blight pathogen, Phytophthora infestans, is spread as a succession of clonal lineages. The discrimination of genetic diversity within such evolving populations provides insights into the processes generating novel lineages and the pathways and drivers of pathogen evolution and dissemination at local and global scales. This knowledge, in turn, helps optimise management practices. Here we combine two key methods for dissecting mitochondrial and nuclear diversity and resolve intra and inter-lineage diversity of over 100 P. infestans isolates representative of key clonal lineages found globally. A novel set of PCR primers that amplify five target regions are provided for mitochondrial DNA sequence analysis. These five loci increased the number of mtDNA haplotypes resolved from four with the PCR RFLP method to 37 (17, 6, 8 and 4 for Ia, Ib, IIa, and IIb haplotypes, respectively, plus 2 Herb-1 haplotypes). As with the PCR RFLP method, two main lineages, I and II were defined. Group I contained 25 mtDNA haplotypes that grouped broadly according to the Ia and Ib types and resolved several sub-clades amongst the global sample. Group II comprised two distinct clusters with four haplotypes corresponding to the RFLP type IIb and eight haplotypes resolved within type IIa. The 12-plex SSR assay revealed 90 multilocus genotypes providing accurate discrimination of dominant clonal lineages and other genetically diverse isolates. Some association of genetic diversity and geographic region of contemporary isolates was observed; US and Mexican isolates formed a loose grouping, distinct from isolates from Europe, South America and other regions. Diversity within clonal lineages was observed that varied according to the age of the clone. In combination, these fine-scale nuclear and maternally inherited mitochondrial markers enabled a greater level of discrimination among isolates than previously available and provided complementary perspectives on evolutionary questions relating to the diversity, phylogeography and the origins and spread of clonal lineages of P. infestans.https://doi.org/10.1371/journal.pone.0208606 |
| spellingShingle | Frank N Martin Yonghong Zhang David E L Cooke Mike D Coffey Niklaus J Grünwald William E Fry Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. PLoS ONE |
| title | Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. |
| title_full | Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. |
| title_fullStr | Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. |
| title_full_unstemmed | Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. |
| title_short | Insights into evolving global populations of Phytophthora infestans via new complementary mtDNA haplotype markers and nuclear SSRs. |
| title_sort | insights into evolving global populations of phytophthora infestans via new complementary mtdna haplotype markers and nuclear ssrs |
| url | https://doi.org/10.1371/journal.pone.0208606 |
| work_keys_str_mv | AT franknmartin insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs AT yonghongzhang insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs AT davidelcooke insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs AT mikedcoffey insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs AT niklausjgrunwald insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs AT williamefry insightsintoevolvingglobalpopulationsofphytophthorainfestansvianewcomplementarymtdnahaplotypemarkersandnuclearssrs |