Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth
Porcine species have a great impact on studies on biomaterial production, organ transplantation and the development of biomedical models. The low efficiency of in vitro-produced embryos to derive embryonic stem cells has made achieving this goal a challenge. The fallopian tube plays an important rol...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/11/609 |
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| author | Xun Fang Bereket Molla Tanga Seonggyu Bang Chaerim Seo Heyyoung Kim Islam M. Saadeldin Sanghoon Lee Jongki Cho |
| author_facet | Xun Fang Bereket Molla Tanga Seonggyu Bang Chaerim Seo Heyyoung Kim Islam M. Saadeldin Sanghoon Lee Jongki Cho |
| author_sort | Xun Fang |
| collection | DOAJ |
| description | Porcine species have a great impact on studies on biomaterial production, organ transplantation and the development of biomedical models. The low efficiency of in vitro-produced embryos to derive embryonic stem cells has made achieving this goal a challenge. The fallopian tube plays an important role in the development of embryos. Extracellular vesicles (EVs) secreted by oviductal epithelial cells play an important role in the epigenetic regulation of embryo development. We used artificially isolated oviductal epithelial cells and EVs. In this study, oviductal epithelial cell (OEC) EVs were isolated and characterized through transmission electron microscopy, nanoparticles tracking analysis, western blotting and proteomics. We found that embryo development and blastocyst formation rate was significantly increased (14.3% ± 0.6% vs. 6.0% ± 0.6%) after OEC EVs treatment. According to our data, the inner cell mass (ICM)/trophectoderm (TE) ratio of the embryonic cell number increased significantly after OEC EVs treatment (43.7% ± 2.3% vs. 28.4% ± 2.1%). Meanwhile, the attachment ability of embryos treated with OEV EVs was significantly improved (43.5% ± 2.1% vs. 29.2% ± 2.5%, respectively). Using quantitative polymerase chain reaction (qPCR), we found that the expression of reprogramming genes (POU5F1, SOX2, NANOG, KLF4 and c-Myc) and implantation-related genes (VIM, KRT8, TEAD4 and CDX2) significantly increased in OEC EV-treated embryos. We report that OEC EV treatment can improve the development and implantation abilities of embryos. |
| first_indexed | 2024-03-09T18:34:37Z |
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| language | English |
| last_indexed | 2024-03-09T18:34:37Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-bcdad4339711431fa33e11ffeb2569672023-11-24T07:14:58ZengMDPI AGVeterinary Sciences2306-73812022-11-0191160910.3390/vetsci9110609Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast OutgrowthXun Fang0Bereket Molla Tanga1Seonggyu Bang2Chaerim Seo3Heyyoung Kim4Islam M. Saadeldin5Sanghoon Lee6Jongki Cho7Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaSchool of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaLaboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaPorcine species have a great impact on studies on biomaterial production, organ transplantation and the development of biomedical models. The low efficiency of in vitro-produced embryos to derive embryonic stem cells has made achieving this goal a challenge. The fallopian tube plays an important role in the development of embryos. Extracellular vesicles (EVs) secreted by oviductal epithelial cells play an important role in the epigenetic regulation of embryo development. We used artificially isolated oviductal epithelial cells and EVs. In this study, oviductal epithelial cell (OEC) EVs were isolated and characterized through transmission electron microscopy, nanoparticles tracking analysis, western blotting and proteomics. We found that embryo development and blastocyst formation rate was significantly increased (14.3% ± 0.6% vs. 6.0% ± 0.6%) after OEC EVs treatment. According to our data, the inner cell mass (ICM)/trophectoderm (TE) ratio of the embryonic cell number increased significantly after OEC EVs treatment (43.7% ± 2.3% vs. 28.4% ± 2.1%). Meanwhile, the attachment ability of embryos treated with OEV EVs was significantly improved (43.5% ± 2.1% vs. 29.2% ± 2.5%, respectively). Using quantitative polymerase chain reaction (qPCR), we found that the expression of reprogramming genes (POU5F1, SOX2, NANOG, KLF4 and c-Myc) and implantation-related genes (VIM, KRT8, TEAD4 and CDX2) significantly increased in OEC EV-treated embryos. We report that OEC EV treatment can improve the development and implantation abilities of embryos.https://www.mdpi.com/2306-7381/9/11/609extracellular vesiclesoviduct epithelial cellsembryonic stem cellsporcineembryo |
| spellingShingle | Xun Fang Bereket Molla Tanga Seonggyu Bang Chaerim Seo Heyyoung Kim Islam M. Saadeldin Sanghoon Lee Jongki Cho Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth Veterinary Sciences extracellular vesicles oviduct epithelial cells embryonic stem cells porcine embryo |
| title | Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth |
| title_full | Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth |
| title_fullStr | Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth |
| title_full_unstemmed | Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth |
| title_short | Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth |
| title_sort | oviduct epithelial cell derived extracellular vesicles improve porcine trophoblast outgrowth |
| topic | extracellular vesicles oviduct epithelial cells embryonic stem cells porcine embryo |
| url | https://www.mdpi.com/2306-7381/9/11/609 |
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