Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates
Introduction: Prolonged and improper use of antibiotics increases the resistance among pathogens and leads to life-threatening implications and increases mortality. The incidence of mupirocin resistance in Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates were reportedly increasin...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2022-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/16298/55943_CE[Ra1]_F[SH]_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
| _version_ | 1811167065115983872 |
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| author | P Ganesh Perumal Subisha Kannan B Appalaraju |
| author_facet | P Ganesh Perumal Subisha Kannan B Appalaraju |
| author_sort | P Ganesh Perumal |
| collection | DOAJ |
| description | Introduction: Prolonged and improper use of antibiotics increases the resistance among pathogens and leads to life-threatening implications and increases mortality. The incidence of mupirocin resistance in Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates were reportedly increasing.
Aim: To determine the rate of high level and low level mupirocin resistance in clinical MRSA isolates in a tertiary care hospital.
Materials and Methods: A cross-sectional study was carried out for a period of three months from December 2019 to February 2020 in Department of Microbiology, PSG Institute of Medical Sciences and Research (PSG IMSR), Coimbatore, Tamil Nadu, India. A total of 100 non duplicate Staphylococcus aureus isolates from different specimens were subjected to mupirocin susceptibility by Kirby-Bauer disc diffusion method as per Clinical and Laboratory Standards Institute (CLSI) 2019 standards. The low (MuL) and high (MuH) level mupirocin resistance were detected by using 5 and 200 μg mupirocin discs (Himedia) respectively. The isolate exhibiting diameter of ≥14 mm indicates its susceptibility. The isolate exhibiting diameter ≤13 mm for both 5 and 200 μg indicates MuL and MuH strains respectively. Pearson’s Chi-square test was calculated and p-value of <0.05 was considered as statistically significant.
Results: Total 51% Staphylococcus aureus isolates were found to be MRSA. In present study, 6 out of 51 (11.8%) MRSA isolates were found to exhibit MuL and 5 out of 51 (9.8%) MRSA isolates were found to be having MuH. Low level and high level mupirocin resistance were not observed in Methicillin Sensitive Staphylococcus aureus (MSSA).
Conclusion: The incidence of MuH and MuL resistance among MRSA were found to be 9.8% and 11.8%, respectively. Screening for mupirocin resistant MRSA to be carried out periodically and stringent infection control practices to be in place to prevent further spread of mupirocin resistant MRSA. |
| first_indexed | 2024-04-10T16:02:55Z |
| format | Article |
| id | doaj.art-bcdd818580b34e6ab54a51299ddada53 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T16:02:55Z |
| publishDate | 2022-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-bcdd818580b34e6ab54a51299ddada532023-02-10T10:15:18ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-05-01165DC06DC1010.7860/JCDR/2022/55943.16298Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus IsolatesP Ganesh Perumal0Subisha Kannan1B Appalaraju2Assistant Professor, Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.Student, Department of Microbiology, San International College of Arts and Science, Coimbatore, Tamil Nadu, India.Professor and Head, Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.Introduction: Prolonged and improper use of antibiotics increases the resistance among pathogens and leads to life-threatening implications and increases mortality. The incidence of mupirocin resistance in Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates were reportedly increasing. Aim: To determine the rate of high level and low level mupirocin resistance in clinical MRSA isolates in a tertiary care hospital. Materials and Methods: A cross-sectional study was carried out for a period of three months from December 2019 to February 2020 in Department of Microbiology, PSG Institute of Medical Sciences and Research (PSG IMSR), Coimbatore, Tamil Nadu, India. A total of 100 non duplicate Staphylococcus aureus isolates from different specimens were subjected to mupirocin susceptibility by Kirby-Bauer disc diffusion method as per Clinical and Laboratory Standards Institute (CLSI) 2019 standards. The low (MuL) and high (MuH) level mupirocin resistance were detected by using 5 and 200 μg mupirocin discs (Himedia) respectively. The isolate exhibiting diameter of ≥14 mm indicates its susceptibility. The isolate exhibiting diameter ≤13 mm for both 5 and 200 μg indicates MuL and MuH strains respectively. Pearson’s Chi-square test was calculated and p-value of <0.05 was considered as statistically significant. Results: Total 51% Staphylococcus aureus isolates were found to be MRSA. In present study, 6 out of 51 (11.8%) MRSA isolates were found to exhibit MuL and 5 out of 51 (9.8%) MRSA isolates were found to be having MuH. Low level and high level mupirocin resistance were not observed in Methicillin Sensitive Staphylococcus aureus (MSSA). Conclusion: The incidence of MuH and MuL resistance among MRSA were found to be 9.8% and 11.8%, respectively. Screening for mupirocin resistant MRSA to be carried out periodically and stringent infection control practices to be in place to prevent further spread of mupirocin resistant MRSA.https://www.jcdr.net/articles/PDF/16298/55943_CE[Ra1]_F[SH]_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdfclinical isolatesinducible clindamycin resistancemupirocin resistant staphylococcus aureus |
| spellingShingle | P Ganesh Perumal Subisha Kannan B Appalaraju Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates Journal of Clinical and Diagnostic Research clinical isolates inducible clindamycin resistance mupirocin resistant staphylococcus aureus |
| title | Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates |
| title_full | Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates |
| title_fullStr | Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates |
| title_full_unstemmed | Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates |
| title_short | Detection and Distribution of Low Level and High Level Mupirocin Resistance among Clinical Methicillin Resistant Staphylococcus aureus Isolates |
| title_sort | detection and distribution of low level and high level mupirocin resistance among clinical methicillin resistant staphylococcus aureus isolates |
| topic | clinical isolates inducible clindamycin resistance mupirocin resistant staphylococcus aureus |
| url | https://www.jcdr.net/articles/PDF/16298/55943_CE[Ra1]_F[SH]_PF1(AG_SS)_PFA(AG_KM)_PN(KM).pdf |
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