Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius)
Background and Aim: Paratuberculosis (PTB) or John’s disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-06-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.16/June-2023/13.pdf |
| _version_ | 1797807135261720576 |
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| author | El Tigani Ahmed El Tigani-Asil Ghada El Derdiri Abdelwahab El Hadi Ahmed Mohamed Abdu Abdelnasir Mohammed Adam Terab Nasareldien Altaib Hussein Khalil Zhaya Jaber Mohammed Al Marri Mohd Farouk Yuosf Asma Abdi Mohamed Shah Abdelmalik Ibrahim Khalafalla Hassan Zackaria Ali Ishag |
| author_facet | El Tigani Ahmed El Tigani-Asil Ghada El Derdiri Abdelwahab El Hadi Ahmed Mohamed Abdu Abdelnasir Mohammed Adam Terab Nasareldien Altaib Hussein Khalil Zhaya Jaber Mohammed Al Marri Mohd Farouk Yuosf Asma Abdi Mohamed Shah Abdelmalik Ibrahim Khalafalla Hassan Zackaria Ali Ishag |
| author_sort | El Tigani Ahmed El Tigani-Asil |
| collection | DOAJ |
| description | Background and Aim: Paratuberculosis (PTB) or John’s disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed to highlight the anatomopathological changes of PTB in camels, which may help in verifying and validating some diagnostic tests used to detect the etiology of the disease in camel tissues.
Materials and Methods: In August 2017, at Alselaa border’s Veterinary Clinic of Al Dhafra Region, Western Abu Dhabi, UAE, one imported culled she-camel of 2 years old was subjected to clinical, microscopic, and anatomopathological investigations along with real-time quantitative polymerase chain reaction (q-PCR) to confirm the infection and correlate between clinical signs and pathological lesions of the PTB in dromedary camels.
Results: Clinically, typical clinical signs compliant with the pathognomonic gross and histologic lesions of PTB were seen in naturally infected dromedary camel. As presumptive diagnosis microscopically, acid-fast coccobacillus bacterium clumps were demonstrated in direct fecal smears as well as in scraped mucosal and crushed mesenteric lymph node films, and in histopathological sections prepared from a necropsied animal and stained by Ziehl-Neelsen stain. Free and intracellular acid-fast clump phagosomes were further confirmed as Mycobacterium avium subsp. paratuberculosis by q-PCR.
Conclusion: Clinical signs and pathological lesions of paratuberculosis in a dromedary camel were found to be similar to those of the other susceptible hosts. |
| first_indexed | 2024-03-13T06:17:59Z |
| format | Article |
| id | doaj.art-bce4a8be398e4f039e110b20408443d2 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-13T06:17:59Z |
| publishDate | 2023-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-bce4a8be398e4f039e110b20408443d22023-06-10T04:47:38ZengVeterinary WorldVeterinary World0972-89882231-09162023-06-011661277128310.14202/vetworld.2023.1277-1283Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius)El Tigani Ahmed El Tigani-Asil0https://orcid.org/0009-0004-5693-7481Ghada El Derdiri Abdelwahab1https://orcid.org/0000-0001-9309-1682El Hadi Ahmed Mohamed Abdu2https://orcid.org/0009-0001-8100-8922Abdelnasir Mohammed Adam Terab3https://orcid.org/0000-0002-2822-8221Nasareldien Altaib Hussein Khalil4https://orcid.org/0009-0006-8129-3483Zhaya Jaber Mohammed Al Marri5https://orcid.org/0009-0004-5571-1667Mohd Farouk Yuosf6https://orcid.org/0000-0001-7210-1589Asma Abdi Mohamed Shah7https://orcid.org/0009-0000-1161-9018Abdelmalik Ibrahim Khalafalla8https://orcid.org/0000-0001-9156-1361Hassan Zackaria Ali Ishag9https://orcid.org/0000-0003-4723-1909Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Extension Services and Animal Health Division, Animal Wealth Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.Background and Aim: Paratuberculosis (PTB) or John’s disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed to highlight the anatomopathological changes of PTB in camels, which may help in verifying and validating some diagnostic tests used to detect the etiology of the disease in camel tissues. Materials and Methods: In August 2017, at Alselaa border’s Veterinary Clinic of Al Dhafra Region, Western Abu Dhabi, UAE, one imported culled she-camel of 2 years old was subjected to clinical, microscopic, and anatomopathological investigations along with real-time quantitative polymerase chain reaction (q-PCR) to confirm the infection and correlate between clinical signs and pathological lesions of the PTB in dromedary camels. Results: Clinically, typical clinical signs compliant with the pathognomonic gross and histologic lesions of PTB were seen in naturally infected dromedary camel. As presumptive diagnosis microscopically, acid-fast coccobacillus bacterium clumps were demonstrated in direct fecal smears as well as in scraped mucosal and crushed mesenteric lymph node films, and in histopathological sections prepared from a necropsied animal and stained by Ziehl-Neelsen stain. Free and intracellular acid-fast clump phagosomes were further confirmed as Mycobacterium avium subsp. paratuberculosis by q-PCR. Conclusion: Clinical signs and pathological lesions of paratuberculosis in a dromedary camel were found to be similar to those of the other susceptible hosts.https://www.veterinaryworld.org/Vol.16/June-2023/13.pdfacid-fast bacteriadromedary camelmycobacteriumparatuberculosis |
| spellingShingle | El Tigani Ahmed El Tigani-Asil Ghada El Derdiri Abdelwahab El Hadi Ahmed Mohamed Abdu Abdelnasir Mohammed Adam Terab Nasareldien Altaib Hussein Khalil Zhaya Jaber Mohammed Al Marri Mohd Farouk Yuosf Asma Abdi Mohamed Shah Abdelmalik Ibrahim Khalafalla Hassan Zackaria Ali Ishag Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) Veterinary World acid-fast bacteria dromedary camel mycobacterium paratuberculosis |
| title | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
| title_full | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
| title_fullStr | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
| title_full_unstemmed | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
| title_short | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
| title_sort | pathological microscopic and molecular diagnosis of paratuberculosis john s disease in naturally infected dromedary camel camelus dromedarius |
| topic | acid-fast bacteria dromedary camel mycobacterium paratuberculosis |
| url | https://www.veterinaryworld.org/Vol.16/June-2023/13.pdf |
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