Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes
Berberine (BBR) ameliorates cellular oxidative stress, apoptosis and autophagy induced by lipid metabolism disorder, however, the molecular mechanism associated with it is not well known. To study the mechanism, we started with m<sup>6</sup>A methylation modification to investigate its r...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2370 |
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| author | Meijuan Zhang Jin Liu Chengbing Yu Shangshang Tang Guangzhen Jiang Jing Zhang Hongcai Zhang Jianxiong Xu Weina Xu |
| author_facet | Meijuan Zhang Jin Liu Chengbing Yu Shangshang Tang Guangzhen Jiang Jing Zhang Hongcai Zhang Jianxiong Xu Weina Xu |
| author_sort | Meijuan Zhang |
| collection | DOAJ |
| description | Berberine (BBR) ameliorates cellular oxidative stress, apoptosis and autophagy induced by lipid metabolism disorder, however, the molecular mechanism associated with it is not well known. To study the mechanism, we started with m<sup>6</sup>A methylation modification to investigate its role in lipid deposition zebrafish hepatocytes (ZFL). The results showed that BBR could change the cellular m<sup>6</sup>A RNA methylation level, increase m<sup>6</sup>A levels of <i>Camk1db</i> gene transcript and alter <i>Camk1db</i> gene mRNA expression. Via knockdown of the <i>Camk1db</i> gene, <i>Camk1db</i> could promote cellular ERK phosphorylation levels. Berberine regulated the expression level of <i>Camk1db</i> mRNA by altering the M<sup>6</sup>A RNA methylation of the Camk1db gene, which further affected the synthesis of calmodulin-dependent protein kinase and activated ERK signaling pathway resulting in changes in downstream physiological indicators including ROS production, cell proliferation, apoptosis and autophagy. In conclusion, berberine could regulate cellular oxidative stress, apoptosis and autophagy by mediating <i>Camk1db</i> m<sup>6</sup>A methylation through the targeting of the <i>Camk1db</i>/ERK pathway in zebrafish-hepatocyte. |
| first_indexed | 2024-03-09T17:24:21Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:24:21Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-bce4b9253c4641189450d804082f0c052023-11-24T12:56:53ZengMDPI AGAntioxidants2076-39212022-11-011112237010.3390/antiox11122370Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-HepatocytesMeijuan Zhang0Jin Liu1Chengbing Yu2Shangshang Tang3Guangzhen Jiang4Jing Zhang5Hongcai Zhang6Jianxiong Xu7Weina Xu8Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaShanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No. 800 Jiangchuan Road, Shanghai 200240, ChinaBerberine (BBR) ameliorates cellular oxidative stress, apoptosis and autophagy induced by lipid metabolism disorder, however, the molecular mechanism associated with it is not well known. To study the mechanism, we started with m<sup>6</sup>A methylation modification to investigate its role in lipid deposition zebrafish hepatocytes (ZFL). The results showed that BBR could change the cellular m<sup>6</sup>A RNA methylation level, increase m<sup>6</sup>A levels of <i>Camk1db</i> gene transcript and alter <i>Camk1db</i> gene mRNA expression. Via knockdown of the <i>Camk1db</i> gene, <i>Camk1db</i> could promote cellular ERK phosphorylation levels. Berberine regulated the expression level of <i>Camk1db</i> mRNA by altering the M<sup>6</sup>A RNA methylation of the Camk1db gene, which further affected the synthesis of calmodulin-dependent protein kinase and activated ERK signaling pathway resulting in changes in downstream physiological indicators including ROS production, cell proliferation, apoptosis and autophagy. In conclusion, berberine could regulate cellular oxidative stress, apoptosis and autophagy by mediating <i>Camk1db</i> m<sup>6</sup>A methylation through the targeting of the <i>Camk1db</i>/ERK pathway in zebrafish-hepatocyte.https://www.mdpi.com/2076-3921/11/12/2370berberinem<sup>6</sup>A methylation<i>Camk1db</i>ERKzebrafish hepatocytes (ZFL) |
| spellingShingle | Meijuan Zhang Jin Liu Chengbing Yu Shangshang Tang Guangzhen Jiang Jing Zhang Hongcai Zhang Jianxiong Xu Weina Xu Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes Antioxidants berberine m<sup>6</sup>A methylation <i>Camk1db</i> ERK zebrafish hepatocytes (ZFL) |
| title | Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes |
| title_full | Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes |
| title_fullStr | Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes |
| title_full_unstemmed | Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes |
| title_short | Berberine Regulation of Cellular Oxidative Stress, Apoptosis and Autophagy by Modulation of m<sup>6</sup>A mRNA Methylation through Targeting the <i>Camk1db</i>/ERK Pathway in Zebrafish-Hepatocytes |
| title_sort | berberine regulation of cellular oxidative stress apoptosis and autophagy by modulation of m sup 6 sup a mrna methylation through targeting the i camk1db i erk pathway in zebrafish hepatocytes |
| topic | berberine m<sup>6</sup>A methylation <i>Camk1db</i> ERK zebrafish hepatocytes (ZFL) |
| url | https://www.mdpi.com/2076-3921/11/12/2370 |
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