Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia
Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2020-12-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/27127 |
| _version_ | 1818822698044751872 |
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| author | Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon |
| author_facet | Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon |
| author_sort | Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon |
| collection | DOAJ |
| description | Bacterial resistance to antibiotics is one of the largest medical concerns
worldwide. One of the bacteria of critical priority is E. coli, since
it presents different resistance mechanisms and some of its strains
have evolved resistance to beta-lactam antibiotics. We characterized 32
antibiotic resistant bacterial isolates from confirmed cases of urinary tract
infections from an array of patients in Nariño, southern Colombia. Macro
and microscopic descriptions of the 32 clinical isolates were conducted.
Resistance profiles, biochemical, and molecular characterization (via 16S
rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed.
All the isolates were identified as E. coli and had resistance to beta-lactams,
aminoglycosides, and fluoroquinolones. This resistance was related to
plasmids carrying the blaTEM, blaSHV1, and blaCTXM1 genes. There
were significant differences between the resistance proportions of the
samples (p value: 0.0000), mainly to penicillin, cefotoxin, and imipenem.
Using ERIC-PCR, four clonal states were evidenced that corroborate
a degree of genetic differentiation within the isolate set. Antibiotic
resistance observed in the isolates is associated with resistance genes
present in the bacterial chromosome and plasmids. |
| first_indexed | 2024-12-18T23:28:13Z |
| format | Article |
| id | doaj.art-bce519f083384edeaa3ea1cf7e53ca75 |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-12-18T23:28:13Z |
| publishDate | 2020-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-bce519f083384edeaa3ea1cf7e53ca752022-12-21T20:47:44ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522020-12-0125346348810.11144/Javeriana.SC25-3.coarCharacterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern ColombiaDeisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon0Grupo de Investigación en Biotecnología Microbiana Universidad de Nariño. Campus Universitario Torobajo, Departamento de Biología, Universidad de Nariño, Pasto, Colombia, Docente Facultad de Ciencias Exactas y Naturales. Grupo de Investigación en Biotecnología Microbiana y GIBIMMA Universidad de Nariño. Campus Universitario Torobajo, Departamento de Biología, Universidad de Nariño, Pasto, Colombia, Ciencias Matemáticas. Docente Facultad de Ciencias Exactas y Naturales. Grupo de Investigación GIBIMMA. Campus Universitario Torobajo, Departamento de Matemáticas, Universidad de Nariño, Pasto, Colombia.Bacterial resistance to antibiotics is one of the largest medical concerns worldwide. One of the bacteria of critical priority is E. coli, since it presents different resistance mechanisms and some of its strains have evolved resistance to beta-lactam antibiotics. We characterized 32 antibiotic resistant bacterial isolates from confirmed cases of urinary tract infections from an array of patients in Nariño, southern Colombia. Macro and microscopic descriptions of the 32 clinical isolates were conducted. Resistance profiles, biochemical, and molecular characterization (via 16S rRNA gene sequencing, ERIC-PCR, and resistance genes) were performed. All the isolates were identified as E. coli and had resistance to beta-lactams, aminoglycosides, and fluoroquinolones. This resistance was related to plasmids carrying the blaTEM, blaSHV1, and blaCTXM1 genes. There were significant differences between the resistance proportions of the samples (p value: 0.0000), mainly to penicillin, cefotoxin, and imipenem. Using ERIC-PCR, four clonal states were evidenced that corroborate a degree of genetic differentiation within the isolate set. Antibiotic resistance observed in the isolates is associated with resistance genes present in the bacterial chromosome and plasmids.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/27127antibiotics; escherichia coli; urinary infections; plasmids and resistance. |
| spellingShingle | Deisy Lorena Guerrero-Ceballos, Edith Mariela Burbano-Rosero, Eduardo Ibargüen Mondragon Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia Universitas Scientiarum antibiotics; escherichia coli; urinary infections; plasmids and resistance. |
| title | Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia |
| title_full | Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia |
| title_fullStr | Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia |
| title_full_unstemmed | Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia |
| title_short | Characterization of antibiotic-resistant Escherichia coli associated with urinary tract infections in Southern Colombia |
| title_sort | characterization of antibiotic resistant escherichia coli associated with urinary tract infections in southern colombia |
| topic | antibiotics; escherichia coli; urinary infections; plasmids and resistance. |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/27127 |
| work_keys_str_mv | AT deisylorenaguerreroceballosedithmarielaburbanoroseroeduardoibarguenmondragon characterizationofantibioticresistantescherichiacoliassociatedwithurinarytractinfectionsinsoutherncolombia |