Coagulatory active constituents of Malus pumila Mill. flowers
Abstract Background The flowers of Malus pumila Mill (Rosaceae) is rich in resources, but lack of medicinal value research. Chemical constituents of the same family M. pumila have coagulatory activity. Considering the coagulatory activity could be beneficial for various cardiovascular diseases, the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-12-01
|
| Series: | Chemistry Central Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13065-018-0490-6 |
| _version_ | 1818365265591664640 |
|---|---|
| author | Zhenhua Yin Yong Zhang Juanjuan Zhang Jinmei Wang Wenyi Kang |
| author_facet | Zhenhua Yin Yong Zhang Juanjuan Zhang Jinmei Wang Wenyi Kang |
| author_sort | Zhenhua Yin |
| collection | DOAJ |
| description | Abstract Background The flowers of Malus pumila Mill (Rosaceae) is rich in resources, but lack of medicinal value research. Chemical constituents of the same family M. pumila have coagulatory activity. Considering the coagulatory activity could be beneficial for various cardiovascular diseases, the aim of this study is to evaluate coagulatory active constituents of M. pumila flowers. Methods Chemical constituents of M. pumila flowers were isolated by various column chromatographies, and their coagulatory activity were evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) in vitro. Results Nine compounds were isolated from M. pumila flowers, and identified as kaempferol-3-O-β-d-glucopyranoside (1), kaempferol-7-O-β-d-glucopyranoside (2), kaempferol-3-O-α-l-arabinofuranoside (3), phloridzin (4), kaempferol (5), phloretin (6), β-sitosterol (7), lupeol (8) and pyracanthoside (9). Compounds 1–9 were isolated from the flowers for the first time, compounds 1, 2 and 9 were isolated from the genus for the first time. Compound 2 could significantly shorten APTT, TT and PT, but significantly decrease the content of FIB. Compound 3 could shorten PT. Compound 4 could significantly shorten TT and PT, but significantly decrease the content of FIB. Compound 5 shortened APTT. Compound 6 and 7 could significantly shorten APTT and PT. Compound 9 was able to prolong TT and decrease the content of FIB, but shorten PT. Conclusions Compounds 2–7 possessed significant procoagulant activity in vitro, compound 9 had anticoagulant activity in vitro, which showed coagulation potential of compounds from M. pumila flowers, as a new source of bioactive molecules for therapeutic purposes. |
| first_indexed | 2024-12-13T22:17:31Z |
| format | Article |
| id | doaj.art-bce5614ee842478095cca6ba0d042c93 |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-13T22:17:31Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-bce5614ee842478095cca6ba0d042c932022-12-21T23:29:28ZengBMCChemistry Central Journal1752-153X2018-12-011211710.1186/s13065-018-0490-6Coagulatory active constituents of Malus pumila Mill. flowersZhenhua Yin0Yong Zhang1Juanjuan Zhang2Jinmei Wang3Wenyi Kang4Zhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology CollegeZhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology CollegeZhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology CollegeJoint International Research Laboratory of Food & Medicine Resource FunctionZhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology CollegeAbstract Background The flowers of Malus pumila Mill (Rosaceae) is rich in resources, but lack of medicinal value research. Chemical constituents of the same family M. pumila have coagulatory activity. Considering the coagulatory activity could be beneficial for various cardiovascular diseases, the aim of this study is to evaluate coagulatory active constituents of M. pumila flowers. Methods Chemical constituents of M. pumila flowers were isolated by various column chromatographies, and their coagulatory activity were evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) in vitro. Results Nine compounds were isolated from M. pumila flowers, and identified as kaempferol-3-O-β-d-glucopyranoside (1), kaempferol-7-O-β-d-glucopyranoside (2), kaempferol-3-O-α-l-arabinofuranoside (3), phloridzin (4), kaempferol (5), phloretin (6), β-sitosterol (7), lupeol (8) and pyracanthoside (9). Compounds 1–9 were isolated from the flowers for the first time, compounds 1, 2 and 9 were isolated from the genus for the first time. Compound 2 could significantly shorten APTT, TT and PT, but significantly decrease the content of FIB. Compound 3 could shorten PT. Compound 4 could significantly shorten TT and PT, but significantly decrease the content of FIB. Compound 5 shortened APTT. Compound 6 and 7 could significantly shorten APTT and PT. Compound 9 was able to prolong TT and decrease the content of FIB, but shorten PT. Conclusions Compounds 2–7 possessed significant procoagulant activity in vitro, compound 9 had anticoagulant activity in vitro, which showed coagulation potential of compounds from M. pumila flowers, as a new source of bioactive molecules for therapeutic purposes.http://link.springer.com/article/10.1186/s13065-018-0490-6Malus pumila MillChemical constituentsCoagulatory activity |
| spellingShingle | Zhenhua Yin Yong Zhang Juanjuan Zhang Jinmei Wang Wenyi Kang Coagulatory active constituents of Malus pumila Mill. flowers Chemistry Central Journal Malus pumila Mill Chemical constituents Coagulatory activity |
| title | Coagulatory active constituents of Malus pumila Mill. flowers |
| title_full | Coagulatory active constituents of Malus pumila Mill. flowers |
| title_fullStr | Coagulatory active constituents of Malus pumila Mill. flowers |
| title_full_unstemmed | Coagulatory active constituents of Malus pumila Mill. flowers |
| title_short | Coagulatory active constituents of Malus pumila Mill. flowers |
| title_sort | coagulatory active constituents of malus pumila mill flowers |
| topic | Malus pumila Mill Chemical constituents Coagulatory activity |
| url | http://link.springer.com/article/10.1186/s13065-018-0490-6 |
| work_keys_str_mv | AT zhenhuayin coagulatoryactiveconstituentsofmaluspumilamillflowers AT yongzhang coagulatoryactiveconstituentsofmaluspumilamillflowers AT juanjuanzhang coagulatoryactiveconstituentsofmaluspumilamillflowers AT jinmeiwang coagulatoryactiveconstituentsofmaluspumilamillflowers AT wenyikang coagulatoryactiveconstituentsofmaluspumilamillflowers |