Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5
Synapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-04-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124713001538 |
| _version_ | 1811285515267211264 |
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| author | Courtney Easley-Neal Javier Fierro, Jr. JoAnn Buchanan Philip Washbourne |
| author_facet | Courtney Easley-Neal Javier Fierro, Jr. JoAnn Buchanan Philip Washbourne |
| author_sort | Courtney Easley-Neal |
| collection | DOAJ |
| description | Synapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during development. We examined the transport and recruitment of synapsin to nascent presynaptic terminals in vivo in the developing zebrafish spinal cord. Synapsin was transported in a transport packet independently of two other presynaptic organelles: synaptic vesicle (SV) protein transport vesicles (STVs) and Piccolo-containing active zone precursor transport vesicles (PTVs). During presynaptic assembly, recruitment of all three transport packets occurred in an ordered sequence: STVs preceded PTVs, which in turn preceded synapsin. Importantly, cyclin-dependent kinase 5 (Cdk5) specifically regulated the late recruitment of synapsin transport packets at synapses. These results point to additional layers of complexity in the established mechanisms of synaptogenesis. |
| first_indexed | 2024-04-13T02:45:19Z |
| format | Article |
| id | doaj.art-bce6f682a2f1455e85cb0a2fb44dff61 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-13T02:45:19Z |
| publishDate | 2013-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bce6f682a2f1455e85cb0a2fb44dff612022-12-22T03:06:04ZengElsevierCell Reports2211-12472013-04-01341199121210.1016/j.celrep.2013.03.031Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5Courtney Easley-Neal0Javier Fierro, Jr.1JoAnn Buchanan2Philip Washbourne3Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USAInstitute of Neuroscience, University of Oregon, Eugene, OR 97403, USADepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USAInstitute of Neuroscience, University of Oregon, Eugene, OR 97403, USASynapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during development. We examined the transport and recruitment of synapsin to nascent presynaptic terminals in vivo in the developing zebrafish spinal cord. Synapsin was transported in a transport packet independently of two other presynaptic organelles: synaptic vesicle (SV) protein transport vesicles (STVs) and Piccolo-containing active zone precursor transport vesicles (PTVs). During presynaptic assembly, recruitment of all three transport packets occurred in an ordered sequence: STVs preceded PTVs, which in turn preceded synapsin. Importantly, cyclin-dependent kinase 5 (Cdk5) specifically regulated the late recruitment of synapsin transport packets at synapses. These results point to additional layers of complexity in the established mechanisms of synaptogenesis.http://www.sciencedirect.com/science/article/pii/S2211124713001538 |
| spellingShingle | Courtney Easley-Neal Javier Fierro, Jr. JoAnn Buchanan Philip Washbourne Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 Cell Reports |
| title | Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 |
| title_full | Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 |
| title_fullStr | Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 |
| title_full_unstemmed | Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 |
| title_short | Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5 |
| title_sort | late recruitment of synapsin to nascent synapses is regulated by cdk5 |
| url | http://www.sciencedirect.com/science/article/pii/S2211124713001538 |
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