Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles
Background and Purpose: Vulvovaginal candidiasis (VVC) is an opportunisticinfection due to Candida species, one of the most common genital tract diseases among reproductive-age women. The present study aimed to investigate the prevalence of VVC among non-pregnant women and identify the epidemiology...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-12-01
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| Series: | Current Medical Mycology |
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| Online Access: | http://cmm.mazums.ac.ir/article_140979_8cbb013eb58472fbedae77f331b4e829.pdf |
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| author | Narges Aslani Roya Kokabi Fatemeh Moradi Kiana Abbasi Narges Vaseghi Mohammad Hosein Afsarian |
| author_facet | Narges Aslani Roya Kokabi Fatemeh Moradi Kiana Abbasi Narges Vaseghi Mohammad Hosein Afsarian |
| author_sort | Narges Aslani |
| collection | DOAJ |
| description | Background and Purpose: Vulvovaginal candidiasis (VVC) is an opportunisticinfection due to Candida species, one of the most common genital tract diseases among reproductive-age women. The present study aimed to investigate the prevalence of VVC among non-pregnant women and identify the epidemiology of the involved Candida species with the evaluation of antifungal susceptibilities.Materials and Methods: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed to identify Candida species isolated from the genital tract of 350 non-pregnant women. Moreover, antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute broth microdilution method guidelines (M27-A3 and M27-S4).Results: Vaginal swab cultures of 119 (34%) women yielded Candida species. Candidaalbicans was the most frequently isolated species (68%), followed by Candida glabrata(19.2%). Voriconazole was the most active drug against all tested isolates showing anMIC50/MIC90 corresponding to 0.016/0.25 µg/mL, followed by posaconazole (0.031/1µg/mL). Overall, resistance rates to fluconazole, itraconazole, and voriconazole were2.4%, 4.8% and, 0.8% respectively. However, posaconazole showed potent in vitro activity against all tested isolates.Conclusion: Results of the current study showed that for the effectual therapeuticoutcome of candidiasis, accurate identification of species, appropriate source control,suitable antifungal regimens, and improved antifungal stewardship are highly recommended for the management and treatment of infection with Candida, like VVC. |
| first_indexed | 2024-04-11T04:11:05Z |
| format | Article |
| id | doaj.art-bce9ddaca788451fa3d409983dfe89d6 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-04-11T04:11:05Z |
| publishDate | 2021-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-bce9ddaca788451fa3d409983dfe89d62023-01-01T06:33:11ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202021-12-017461110.18502/cmm.7.4.8405140979Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profilesNarges Aslani0Roya Kokabi1Fatemeh Moradi2Kiana Abbasi3Narges Vaseghi4Mohammad Hosein Afsarian5Infectious and Tropical Diseases Research Centre, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Obstetrics and Gynecology, School of Medicine, Fasa University of Medical Sciences, Fasa, IranDepartment of Medical Mycology and Parasitology, School of Medicine, Fasa University of Medical Sciences, Fasa, IranDepartment of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, IranDepartment of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Medical Mycology and Parasitology, School of Medicine, Fasa University of Medical Sciences, Fasa, IranBackground and Purpose: Vulvovaginal candidiasis (VVC) is an opportunisticinfection due to Candida species, one of the most common genital tract diseases among reproductive-age women. The present study aimed to investigate the prevalence of VVC among non-pregnant women and identify the epidemiology of the involved Candida species with the evaluation of antifungal susceptibilities.Materials and Methods: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed to identify Candida species isolated from the genital tract of 350 non-pregnant women. Moreover, antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute broth microdilution method guidelines (M27-A3 and M27-S4).Results: Vaginal swab cultures of 119 (34%) women yielded Candida species. Candidaalbicans was the most frequently isolated species (68%), followed by Candida glabrata(19.2%). Voriconazole was the most active drug against all tested isolates showing anMIC50/MIC90 corresponding to 0.016/0.25 µg/mL, followed by posaconazole (0.031/1µg/mL). Overall, resistance rates to fluconazole, itraconazole, and voriconazole were2.4%, 4.8% and, 0.8% respectively. However, posaconazole showed potent in vitro activity against all tested isolates.Conclusion: Results of the current study showed that for the effectual therapeuticoutcome of candidiasis, accurate identification of species, appropriate source control,suitable antifungal regimens, and improved antifungal stewardship are highly recommended for the management and treatment of infection with Candida, like VVC.http://cmm.mazums.ac.ir/article_140979_8cbb013eb58472fbedae77f331b4e829.pdfantifungal susceptibilitycandida speciesmaldi-tofvulvovaginal candidiasis |
| spellingShingle | Narges Aslani Roya Kokabi Fatemeh Moradi Kiana Abbasi Narges Vaseghi Mohammad Hosein Afsarian Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles Current Medical Mycology antifungal susceptibility candida species maldi-tof vulvovaginal candidiasis |
| title | Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles |
| title_full | Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles |
| title_fullStr | Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles |
| title_full_unstemmed | Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles |
| title_short | Characterization of Candida species isolated from vulvovaginal candidiasis by MALDI-TOF with in vitro antifungal susceptibility profiles |
| title_sort | characterization of candida species isolated from vulvovaginal candidiasis by maldi tof with in vitro antifungal susceptibility profiles |
| topic | antifungal susceptibility candida species maldi-tof vulvovaginal candidiasis |
| url | http://cmm.mazums.ac.ir/article_140979_8cbb013eb58472fbedae77f331b4e829.pdf |
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