Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli.
Integrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) t...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0233315 |
| _version_ | 1818588667611971584 |
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| author | Susanne Sütterlin James E Bray Martin C J Maiden Eva Tano |
| author_facet | Susanne Sütterlin James E Bray Martin C J Maiden Eva Tano |
| author_sort | Susanne Sütterlin |
| collection | DOAJ |
| description | Integrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) the Murray collection (n = 58 bacteria isolated from the 1910s to 1940s); (ii) the E. coli reference (ECOR) collection (n = 37 isolates mainly from the 1980s); and (iii) a recently assembled collection (n = 88 isolates obtained in 2016). High-quality whole genome sequences (WGSs) were available for all isolates. Integrons were detected in the WGSs with the program IntegronFinder and the results compared with three established methods: (i) polymerase chain reaction detection of the integrase gene; (ii) BLAST searching using draft genomes; and (iii) mapping of short reads. No integrons were found in any of the Murray Collection isolates; however, integrons were present in 3% of the isolates from ECOR collection, assembled in the 1980s, and 26% of the isolates from the 2010s. Similarly, antimicrobial resistance determinants were not present in the Murray Collection isolates, whereas they were present in 19% of the ECOR Collection isolates and in 55% of the isolates obtained in during the 2010s. |
| first_indexed | 2024-12-16T09:28:24Z |
| format | Article |
| id | doaj.art-bcec3d52e2384d71864f9e40a40073dc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:28:24Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bcec3d52e2384d71864f9e40a40073dc2022-12-21T22:36:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023331510.1371/journal.pone.0233315Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli.Susanne SütterlinJames E BrayMartin C J MaidenEva TanoIntegrons play a major role in the evolution and spread of antimicrobial resistance in human pathogens, including Escherichia coli. This study describes the occurrence of class 1 integrons in human pathogenic E. coli, in three isolate collections involving three periods from the last 100 years (i) the Murray collection (n = 58 bacteria isolated from the 1910s to 1940s); (ii) the E. coli reference (ECOR) collection (n = 37 isolates mainly from the 1980s); and (iii) a recently assembled collection (n = 88 isolates obtained in 2016). High-quality whole genome sequences (WGSs) were available for all isolates. Integrons were detected in the WGSs with the program IntegronFinder and the results compared with three established methods: (i) polymerase chain reaction detection of the integrase gene; (ii) BLAST searching using draft genomes; and (iii) mapping of short reads. No integrons were found in any of the Murray Collection isolates; however, integrons were present in 3% of the isolates from ECOR collection, assembled in the 1980s, and 26% of the isolates from the 2010s. Similarly, antimicrobial resistance determinants were not present in the Murray Collection isolates, whereas they were present in 19% of the ECOR Collection isolates and in 55% of the isolates obtained in during the 2010s.https://doi.org/10.1371/journal.pone.0233315 |
| spellingShingle | Susanne Sütterlin James E Bray Martin C J Maiden Eva Tano Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. PLoS ONE |
| title | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. |
| title_full | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. |
| title_fullStr | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. |
| title_full_unstemmed | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. |
| title_short | Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli. |
| title_sort | distribution of class 1 integrons in historic and contemporary collections of human pathogenic escherichia coli |
| url | https://doi.org/10.1371/journal.pone.0233315 |
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