Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation

Microalgae have been viewed as one of potential solution for CO2 biofixation or CO2 sequestration. However, many factors need to be evaluated to support development of CO2 biofixation. One important environmental factor for the growth of micro algae is related with light requirement. The aim of this...

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Main Authors: Johar Gunawan Teuku, Ikhwan Yusni, Restuhadi Fajar, Pato Usman
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:E3S Web of Conferences
Online Access:https://doi.org/10.1051/e3sconf/20183103003
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author Johar Gunawan Teuku
Ikhwan Yusni
Restuhadi Fajar
Pato Usman
author_facet Johar Gunawan Teuku
Ikhwan Yusni
Restuhadi Fajar
Pato Usman
author_sort Johar Gunawan Teuku
collection DOAJ
description Microalgae have been viewed as one of potential solution for CO2 biofixation or CO2 sequestration. However, many factors need to be evaluated to support development of CO2 biofixation. One important environmental factor for the growth of micro algae is related with light requirement. The aim of this study was to evaluate the effect of light intensity and photoperiod on growth of Chlorellapyrenoidosa (C.pyrenoidosa) and CO2 biofixation. Experiments were carried out in 1000 mL semi batch photo bioreactors, purged continuously with air (0.034% CO2). An Experiment of Factorial Design was employed in which the light intensity was evaluated 4 level at 2000, 4000, 6000 and 8000 lux with 3 level of photo period at L/D (light /dark) 8 hours/16 hours; L/D 12 hours/12 hours and L/D 16 hours/8 hours. The result indicated that both light intensity and photo period had significant effect (p< 0.05) on growth of C. pyrenoidosa. However, the photo period showed stronger effect relative to light intensity on growth of C.pyrenoidosa within the range reviewed. The interaction between the two factors was indicative but statistically not significant. Best growth profile sustained at combination of L/D 16 hours/8 hours of photoperiod and light intensity of 8000 lux with the highest average biomass observed at 0.516 ± 0.069gr/L. An increase in CO2biofixation rate of around 2 times was also observed between highest setting (8000 lux; L/D 16/12 hours) relative to that of lowest setting (2000 lux; L/D 8/12 hours).
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spelling doaj.art-bcec55a51f7e46cf82d812ce3415d7272022-12-21T22:01:38ZengEDP SciencesE3S Web of Conferences2267-12422018-01-01310300310.1051/e3sconf/20183103003e3sconf_icenis2018_03003Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 BiofixationJohar Gunawan TeukuIkhwan YusniRestuhadi FajarPato UsmanMicroalgae have been viewed as one of potential solution for CO2 biofixation or CO2 sequestration. However, many factors need to be evaluated to support development of CO2 biofixation. One important environmental factor for the growth of micro algae is related with light requirement. The aim of this study was to evaluate the effect of light intensity and photoperiod on growth of Chlorellapyrenoidosa (C.pyrenoidosa) and CO2 biofixation. Experiments were carried out in 1000 mL semi batch photo bioreactors, purged continuously with air (0.034% CO2). An Experiment of Factorial Design was employed in which the light intensity was evaluated 4 level at 2000, 4000, 6000 and 8000 lux with 3 level of photo period at L/D (light /dark) 8 hours/16 hours; L/D 12 hours/12 hours and L/D 16 hours/8 hours. The result indicated that both light intensity and photo period had significant effect (p< 0.05) on growth of C. pyrenoidosa. However, the photo period showed stronger effect relative to light intensity on growth of C.pyrenoidosa within the range reviewed. The interaction between the two factors was indicative but statistically not significant. Best growth profile sustained at combination of L/D 16 hours/8 hours of photoperiod and light intensity of 8000 lux with the highest average biomass observed at 0.516 ± 0.069gr/L. An increase in CO2biofixation rate of around 2 times was also observed between highest setting (8000 lux; L/D 16/12 hours) relative to that of lowest setting (2000 lux; L/D 8/12 hours).https://doi.org/10.1051/e3sconf/20183103003
spellingShingle Johar Gunawan Teuku
Ikhwan Yusni
Restuhadi Fajar
Pato Usman
Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
E3S Web of Conferences
title Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
title_full Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
title_fullStr Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
title_full_unstemmed Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
title_short Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation
title_sort effect of light intensity and photoperiod on growth of chlorella pyrenoidosa and co2 biofixation
url https://doi.org/10.1051/e3sconf/20183103003
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