Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis
Abstract Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant...
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| Format: | Article |
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SpringerOpen
2023-02-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-023-00362-z |
| _version_ | 1797863893055307776 |
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| author | Zienab Ali Tarek Dishisha Ahmed O. El-Gendy Ahmed F. Azmy |
| author_facet | Zienab Ali Tarek Dishisha Ahmed O. El-Gendy Ahmed F. Azmy |
| author_sort | Zienab Ali |
| collection | DOAJ |
| description | Abstract Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. |
| first_indexed | 2024-04-09T22:42:58Z |
| format | Article |
| id | doaj.art-bcecb6a18a1b41c9b20c7fdfbe7fb0a5 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-04-09T22:42:58Z |
| publishDate | 2023-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-bcecb6a18a1b41c9b20c7fdfbe7fb0a52023-03-22T12:03:33ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432023-02-0112111010.1186/s43088-023-00362-zIsolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalisZienab Ali0Tarek Dishisha1Ahmed O. El-Gendy2Ahmed F. Azmy3Department of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef UniversityAbstract Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms.https://doi.org/10.1186/s43088-023-00362-zBacteriophageResistanceEnterococcus faecalisBiofilm |
| spellingShingle | Zienab Ali Tarek Dishisha Ahmed O. El-Gendy Ahmed F. Azmy Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis Beni-Suef University Journal of Basic and Applied Sciences Bacteriophage Resistance Enterococcus faecalis Biofilm |
| title | Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| title_full | Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| title_fullStr | Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| title_full_unstemmed | Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| title_short | Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| title_sort | isolation and phenotypic characterization of bacteriophage sa14 with lytic and anti biofilm activity against multidrug resistant enterococcus faecalis |
| topic | Bacteriophage Resistance Enterococcus faecalis Biofilm |
| url | https://doi.org/10.1186/s43088-023-00362-z |
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