Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus
<p>Abstract</p> <p>Background</p> <p>Marek’s disease virus (MDV) is a highly cell-associated oncogenic α-herpesvirus that causes a disease characterised by T-cell lymphomas. The pathogenesis, or the nature of the interaction of the virus and the host, in the thymus are...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-11-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/9/1/256 |
| _version_ | 1818916381609951232 |
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| author | Hu Xuming Qin Aijian Qian Kun Shao Hongxia Yu Chuan Xu Wencai Miao Ji |
| author_facet | Hu Xuming Qin Aijian Qian Kun Shao Hongxia Yu Chuan Xu Wencai Miao Ji |
| author_sort | Hu Xuming |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Marek’s disease virus (MDV) is a highly cell-associated oncogenic α-herpesvirus that causes a disease characterised by T-cell lymphomas. The pathogenesis, or the nature of the interaction of the virus and the host, in the thymus are still unclear.</p> <p>Results</p> <p>In this study, we identified 119 differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry from the thymuses of chickens infected with the RB1B strain of MDV. These differentially expressed proteins were found mainly at 21, 28 and 35 days post-infection. More than 20 of the differentially expressed proteins were directly associated with immunity, apoptosis, tumour development and viral infection and replication. Five of these proteins, ANXA1, MIF, NPM1, OP18 and VIM, were further confirmed using real-time PCR. The functional associations and roles in oncogenesis of these proteins are discussed.</p> <p>Conclusions</p> <p>This work provides a proteomic profiling of host responses to MDV in the thymus of chickens and further characterises proteins related to the mechanisms of MDV oncogenesis and pathogenesis.</p> |
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| format | Article |
| id | doaj.art-bceea34e6e4348a7b4a117958ee1bf56 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-20T00:17:16Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-bceea34e6e4348a7b4a117958ee1bf562022-12-21T20:00:17ZengBMCVirology Journal1743-422X2012-11-019125610.1186/1743-422X-9-256Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virusHu XumingQin AijianQian KunShao HongxiaYu ChuanXu WencaiMiao Ji<p>Abstract</p> <p>Background</p> <p>Marek’s disease virus (MDV) is a highly cell-associated oncogenic α-herpesvirus that causes a disease characterised by T-cell lymphomas. The pathogenesis, or the nature of the interaction of the virus and the host, in the thymus are still unclear.</p> <p>Results</p> <p>In this study, we identified 119 differentially expressed proteins using two-dimensional electrophoresis and mass spectrometry from the thymuses of chickens infected with the RB1B strain of MDV. These differentially expressed proteins were found mainly at 21, 28 and 35 days post-infection. More than 20 of the differentially expressed proteins were directly associated with immunity, apoptosis, tumour development and viral infection and replication. Five of these proteins, ANXA1, MIF, NPM1, OP18 and VIM, were further confirmed using real-time PCR. The functional associations and roles in oncogenesis of these proteins are discussed.</p> <p>Conclusions</p> <p>This work provides a proteomic profiling of host responses to MDV in the thymus of chickens and further characterises proteins related to the mechanisms of MDV oncogenesis and pathogenesis.</p>http://www.virologyj.com/content/9/1/256 |
| spellingShingle | Hu Xuming Qin Aijian Qian Kun Shao Hongxia Yu Chuan Xu Wencai Miao Ji Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus Virology Journal |
| title | Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus |
| title_full | Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus |
| title_fullStr | Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus |
| title_full_unstemmed | Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus |
| title_short | Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus |
| title_sort | analysis of protein expression profiles in the thymus of chickens infected with marek s disease virus |
| url | http://www.virologyj.com/content/9/1/256 |
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