Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan
Background: We performed molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan to reveal the risk of C. difficile infection. Methods: Cultured isolates from 919 stool samples from 869 patients obtained from July 2015 to August 2016 were subjected t...
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Elsevier
2023-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023073759 |
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| author | Yukitaka Ito Koichi Tanimoto Naoko Chiba Masanobu Otsuka Masato Ota Mieko Yoshida Yusuke Hashimoto Takahiro Nomura Haruyoshi Tomita |
| author_facet | Yukitaka Ito Koichi Tanimoto Naoko Chiba Masanobu Otsuka Masato Ota Mieko Yoshida Yusuke Hashimoto Takahiro Nomura Haruyoshi Tomita |
| author_sort | Yukitaka Ito |
| collection | DOAJ |
| description | Background: We performed molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan to reveal the risk of C. difficile infection. Methods: Cultured isolates from 919 stool samples from 869 patients obtained from July 2015 to August 2016 were subjected to toxin gene detection, ribotyping, multilocus sequence typing, antimicrobial susceptibility testing, and quantitative real-time polymerase chain reaction testing for C. difficile toxin gene expression. Results: Of the 919 stool samples from 869 patients, C. difficile was isolated from 153 samples (16.6%), of which 49 (32%) and 104 (68%) were from patients with and without C. difficile infection, respectively. Analyses showed genetic diversity, with ST8 and ST17 strains of healthcare-associated infections, some of which caused C. difficile infections. There was no significant difference in the transcription levels of C. difficile toxin genes between isolates from patients with and without C. difficile infection. Conclusions: Major Japanese clonal strains, ST8 and ST17, have been in the hospital environment for a long time and cause healthcare-associated C. difficile infections. The C. difficile toxin genes were transcribed in the isolates from both patients with and without C. difficile infection but were no significant relationship with the development of C. difficile infection. |
| first_indexed | 2024-03-11T15:04:08Z |
| format | Article |
| id | doaj.art-bcf2c5422e1a4dfaa318f844c34b55be |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T15:04:08Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bcf2c5422e1a4dfaa318f844c34b55be2023-10-30T06:05:27ZengElsevierHeliyon2405-84402023-10-01910e20167Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in JapanYukitaka Ito0Koichi Tanimoto1Naoko Chiba2Masanobu Otsuka3Masato Ota4Mieko Yoshida5Yusuke Hashimoto6Takahiro Nomura7Haruyoshi Tomita8Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, Japan; Department of Bacteriology, Gunma University Graduate School of Medicine, Gunma, Japan; Corresponding author. Division of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, Japan.Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, Gunma, JapanDivision of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, JapanDivision of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, JapanDivision of Clinical Microbiology Laboratory, Toho University Ohashi Medical Center, Tokyo, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, Gunma, JapanDepartment of Bacteriology, Gunma University Graduate School of Medicine, Gunma, Japan; Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Gunma, JapanBackground: We performed molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan to reveal the risk of C. difficile infection. Methods: Cultured isolates from 919 stool samples from 869 patients obtained from July 2015 to August 2016 were subjected to toxin gene detection, ribotyping, multilocus sequence typing, antimicrobial susceptibility testing, and quantitative real-time polymerase chain reaction testing for C. difficile toxin gene expression. Results: Of the 919 stool samples from 869 patients, C. difficile was isolated from 153 samples (16.6%), of which 49 (32%) and 104 (68%) were from patients with and without C. difficile infection, respectively. Analyses showed genetic diversity, with ST8 and ST17 strains of healthcare-associated infections, some of which caused C. difficile infections. There was no significant difference in the transcription levels of C. difficile toxin genes between isolates from patients with and without C. difficile infection. Conclusions: Major Japanese clonal strains, ST8 and ST17, have been in the hospital environment for a long time and cause healthcare-associated C. difficile infections. The C. difficile toxin genes were transcribed in the isolates from both patients with and without C. difficile infection but were no significant relationship with the development of C. difficile infection.http://www.sciencedirect.com/science/article/pii/S2405844023073759Clostridioides difficileMolecular epidemiologyCD toxinRibotypingHealthcare-associated infection |
| spellingShingle | Yukitaka Ito Koichi Tanimoto Naoko Chiba Masanobu Otsuka Masato Ota Mieko Yoshida Yusuke Hashimoto Takahiro Nomura Haruyoshi Tomita Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan Heliyon Clostridioides difficile Molecular epidemiology CD toxin Ribotyping Healthcare-associated infection |
| title | Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan |
| title_full | Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan |
| title_fullStr | Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan |
| title_full_unstemmed | Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan |
| title_short | Molecular epidemiological analyses of Clostridioides difficile isolates in a university hospital in Japan |
| title_sort | molecular epidemiological analyses of clostridioides difficile isolates in a university hospital in japan |
| topic | Clostridioides difficile Molecular epidemiology CD toxin Ribotyping Healthcare-associated infection |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023073759 |
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