Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages
This study aims to formulate naringenin (Nar)-loaded pH/temperature sensitive nanopolymers for therapeutic options for inflammatory diseases. For this aim, Nar loaded SNPs (NarSNPs) were synthesized by miniememulsion method and Scanning electron microscopy (SEM), Fourier-Transform Infrared Spectrosc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Applied Surface Science Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666523923001617 |
| _version_ | 1797389434309574656 |
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| author | Metin Yildirim Ömür Acet |
| author_facet | Metin Yildirim Ömür Acet |
| author_sort | Metin Yildirim |
| collection | DOAJ |
| description | This study aims to formulate naringenin (Nar)-loaded pH/temperature sensitive nanopolymers for therapeutic options for inflammatory diseases. For this aim, Nar loaded SNPs (NarSNPs) were synthesized by miniememulsion method and Scanning electron microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR) and, Dynamic Light Scattering (DLS) characterization experiments were conducted. The cytokines release (IL-6 and TNF-α) and cell viability were determined on lipopolysaccharide (LPS) induced RAW 264.7 macrophages. SNPs were determined as a potential naringenin carrier in terms of loading capacity, entrapment efficiency, pH/temperature dependent release, and preservation of bioactivity of Nar. According to our cell viability analyses, NarSNPs are safe at concentration 120 µg/mL. pH/temperature sensitive SNPs decreased the levels of the proinflammatory cytokines IL-6, TNF-α 3.95 and 4.35 times more efficiently than free Nar, respectively. These results indicate that SNPs increase bioactivity of Nar against adverse effects of inflammatory response. |
| first_indexed | 2024-03-08T22:55:55Z |
| format | Article |
| id | doaj.art-bcf3f3071ce2452aadbc3ec63d2cef91 |
| institution | Directory Open Access Journal |
| issn | 2666-5239 |
| language | English |
| last_indexed | 2024-03-08T22:55:55Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Surface Science Advances |
| spelling | doaj.art-bcf3f3071ce2452aadbc3ec63d2cef912023-12-16T06:09:36ZengElsevierApplied Surface Science Advances2666-52392023-12-0118100527Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophagesMetin Yildirim0Ömür Acet1Department of Biochemistry, Faculty of Pharmacy, Harran University, Sanliurfa, Turkey; Corresponding author.Department of Pharmacy Services, Vocational School of Health Services, Tarsus University, Mersin, TurkeyThis study aims to formulate naringenin (Nar)-loaded pH/temperature sensitive nanopolymers for therapeutic options for inflammatory diseases. For this aim, Nar loaded SNPs (NarSNPs) were synthesized by miniememulsion method and Scanning electron microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR) and, Dynamic Light Scattering (DLS) characterization experiments were conducted. The cytokines release (IL-6 and TNF-α) and cell viability were determined on lipopolysaccharide (LPS) induced RAW 264.7 macrophages. SNPs were determined as a potential naringenin carrier in terms of loading capacity, entrapment efficiency, pH/temperature dependent release, and preservation of bioactivity of Nar. According to our cell viability analyses, NarSNPs are safe at concentration 120 µg/mL. pH/temperature sensitive SNPs decreased the levels of the proinflammatory cytokines IL-6, TNF-α 3.95 and 4.35 times more efficiently than free Nar, respectively. These results indicate that SNPs increase bioactivity of Nar against adverse effects of inflammatory response.http://www.sciencedirect.com/science/article/pii/S2666523923001617NaringeninSmart nanopolymersTemperature sensitive polymerpH sensitive polymerRaw 264.7 cells |
| spellingShingle | Metin Yildirim Ömür Acet Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages Applied Surface Science Advances Naringenin Smart nanopolymers Temperature sensitive polymer pH sensitive polymer Raw 264.7 cells |
| title | Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages |
| title_full | Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages |
| title_fullStr | Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages |
| title_full_unstemmed | Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages |
| title_short | Immunomodulatory activities of pH/temperature sensitive smart naringenin-loaded nanopolymers on the mammalian macrophages |
| title_sort | immunomodulatory activities of ph temperature sensitive smart naringenin loaded nanopolymers on the mammalian macrophages |
| topic | Naringenin Smart nanopolymers Temperature sensitive polymer pH sensitive polymer Raw 264.7 cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666523923001617 |
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