Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2
The present study aimed to investigate the effects of <i>Moringa oleifera</i> leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against <i>Streptococcus agalactiae</i> Biotype 2. F...
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MDPI AG
2024-03-01
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| author | Manoj Tukaram Kamble Wenresti Gallardo Krishna Rugmini Salin Suntree Pumpuang Balasaheb Ramdas Chavan Ram Chandra Bhujel Seema Vijay Medhe Aikkarach Kettawan Kim D. Thompson Nopadon Pirarat |
| author_facet | Manoj Tukaram Kamble Wenresti Gallardo Krishna Rugmini Salin Suntree Pumpuang Balasaheb Ramdas Chavan Ram Chandra Bhujel Seema Vijay Medhe Aikkarach Kettawan Kim D. Thompson Nopadon Pirarat |
| author_sort | Manoj Tukaram Kamble |
| collection | DOAJ |
| description | The present study aimed to investigate the effects of <i>Moringa oleifera</i> leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against <i>Streptococcus agalactiae</i> Biotype 2. Four hundred and fifty Nile tilapia (32.61 ± 0.2 g/fish) were randomly allocated into fifteen tanks (30 fish/tank). Different concentrations of MLE at 0%, 0.5%, 1%, 1.5%, and 2% were fed to the Nile tilapia for 30 days, and the growth, feed utilization, hematology, and innate immune response of the Nile tilapia were determined. After the feeding trial, the Nile tilapia were challenged with a <i>S. agalactiae</i> Biotype 2 infection, and the relative percentage of survival (RPS) was determined. Results revealed the presence of quercetin, kaempferol, and <i>p</i>-coumaric acid in the MLE extract, exhibiting stronger antimicrobial activity against <i>S. agalactiae</i> Biotype 2. The diets supplemented with the MLE-0.5 group showed a significantly higher growth, feed utilization, hematology, and innate immune response in the Nile tilapia compared to the control and other MLE groups. Additionally, the MLE-0.5 group exhibited a significantly higher RPS of the Nile tilapia against <i>S. agalactiae</i> Biotype 2. Therefore, MLE-0.5 can be employed as an alternative feed supplement in sustainable Nile tilapia farming to protect against <i>S. agalactiae</i> Biotype 2. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-24T18:38:03Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-bcf6a2ed8baa40bb96c736b5a85a19e92024-03-27T13:17:56ZengMDPI AGAnimals2076-26152024-03-0114695310.3390/ani14060953Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2Manoj Tukaram Kamble0Wenresti Gallardo1Krishna Rugmini Salin2Suntree Pumpuang3Balasaheb Ramdas Chavan4Ram Chandra Bhujel5Seema Vijay Medhe6Aikkarach Kettawan7Kim D. Thompson8Nopadon Pirarat9Center of Excellence in Wildlife, Exotic, and Aquatic Animal Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Marine Science and Fisheries, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat 123, OmanAquaculture and Aquatic Resources Management (AARM), Department of Food, Agriculture and Bioresources, School of Environment Resources and Development, Asian Institute of Technology, Pathum Thani 12120, ThailandAquaculture and Aquatic Resources Management (AARM), Department of Food, Agriculture and Bioresources, School of Environment Resources and Development, Asian Institute of Technology, Pathum Thani 12120, ThailandDepartment of Aquaculture, College of Fisheries, Ratnagiri 415629, IndiaAquaculture and Aquatic Resources Management (AARM), Department of Food, Agriculture and Bioresources, School of Environment Resources and Development, Asian Institute of Technology, Pathum Thani 12120, ThailandCenter of Excellence in Wildlife, Exotic, and Aquatic Animal Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Food Chemistry, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, ThailandMoredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UKCenter of Excellence in Wildlife, Exotic, and Aquatic Animal Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandThe present study aimed to investigate the effects of <i>Moringa oleifera</i> leaf (MLE) extract-supplemented diets on the growth, feed utilization, hematology, innate immune response, and disease resistance of Nile tilapia against <i>Streptococcus agalactiae</i> Biotype 2. Four hundred and fifty Nile tilapia (32.61 ± 0.2 g/fish) were randomly allocated into fifteen tanks (30 fish/tank). Different concentrations of MLE at 0%, 0.5%, 1%, 1.5%, and 2% were fed to the Nile tilapia for 30 days, and the growth, feed utilization, hematology, and innate immune response of the Nile tilapia were determined. After the feeding trial, the Nile tilapia were challenged with a <i>S. agalactiae</i> Biotype 2 infection, and the relative percentage of survival (RPS) was determined. Results revealed the presence of quercetin, kaempferol, and <i>p</i>-coumaric acid in the MLE extract, exhibiting stronger antimicrobial activity against <i>S. agalactiae</i> Biotype 2. The diets supplemented with the MLE-0.5 group showed a significantly higher growth, feed utilization, hematology, and innate immune response in the Nile tilapia compared to the control and other MLE groups. Additionally, the MLE-0.5 group exhibited a significantly higher RPS of the Nile tilapia against <i>S. agalactiae</i> Biotype 2. Therefore, MLE-0.5 can be employed as an alternative feed supplement in sustainable Nile tilapia farming to protect against <i>S. agalactiae</i> Biotype 2.https://www.mdpi.com/2076-2615/14/6/953<i>Moringa oleifera</i>Nile tilapiagrowth performancehematologyimmune response<i>Streptococcus agalactiae</i> Biotype 2 |
| spellingShingle | Manoj Tukaram Kamble Wenresti Gallardo Krishna Rugmini Salin Suntree Pumpuang Balasaheb Ramdas Chavan Ram Chandra Bhujel Seema Vijay Medhe Aikkarach Kettawan Kim D. Thompson Nopadon Pirarat Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 Animals <i>Moringa oleifera</i> Nile tilapia growth performance hematology immune response <i>Streptococcus agalactiae</i> Biotype 2 |
| title | Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 |
| title_full | Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 |
| title_fullStr | Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 |
| title_full_unstemmed | Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 |
| title_short | Effect of <i>Moringa oleifera</i> Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (<i>Oreochromis niloticus</i>) against <i>Streptococcus agalactiae</i> Biotype 2 |
| title_sort | effect of i moringa oleifera i leaf extract on the growth performance hematology innate immunity and disease resistance of nile tilapia i oreochromis niloticus i against i streptococcus agalactiae i biotype 2 |
| topic | <i>Moringa oleifera</i> Nile tilapia growth performance hematology immune response <i>Streptococcus agalactiae</i> Biotype 2 |
| url | https://www.mdpi.com/2076-2615/14/6/953 |
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