Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression
The biocontrol bacterium Paenibacillus alvei K165 has the ability to protect Arabidopsis thaliana against Verticillium dahliae. A direct antagonistic action of strain K165 against V. dahliae was ruled out, making it likely that K165-mediated protection results from induced systemic resistance (ISR)...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2005-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0555 |
| _version_ | 1811278136433704960 |
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| author | Sotirios E. Tjamos Emmanouil Flemetakis Epaminondas J. Paplomatas Panagiotis Katinakis |
| author_facet | Sotirios E. Tjamos Emmanouil Flemetakis Epaminondas J. Paplomatas Panagiotis Katinakis |
| author_sort | Sotirios E. Tjamos |
| collection | DOAJ |
| description | The biocontrol bacterium Paenibacillus alvei K165 has the ability to protect Arabidopsis thaliana against Verticillium dahliae. A direct antagonistic action of strain K165 against V. dahliae was ruled out, making it likely that K165-mediated protection results from induced systemic resistance (ISR) in the host. K165-mediated protection was tested in various Arabidopsis mutants and transgenic plants impaired in defense signaling pathways, including NahG (transgenic line degrading salicylic acid [SA]), etr1-1 (insensitive to ethylene), jar1-1 (insensitive to jasmonate), npr1-1 (non-expressing NPR1 protein), pad3-1 (phytoalexin deficient), pad4-1 (phytoalexin deficient), eds5/sid1 (enhanced disease susceptibility), and sid2 (SA-induction deficient). ISR was blocked in blocked in Arabidopsis mutants npr1-1, eds5/sid1, and sid2, indicating that components of the pathway from isochorismate and a functional NPR1 play a crucial role in the K165-mediated ISR. Furthermore, the concomitant activation and increased transient accumulation of the PR-1, PR-2, and PR-5 genes were observed in the treatment in which both the inducing bacterial strain and the challenging pathogen were present in the rhizosphere of the A. thaliana plants. |
| first_indexed | 2024-04-13T00:29:15Z |
| format | Article |
| id | doaj.art-bcf9fa2ccec54d6a94e98cac4fa54e1e |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:29:15Z |
| publishDate | 2005-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-bcf9fa2ccec54d6a94e98cac4fa54e1e2022-12-22T03:10:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062005-06-0118655556110.1094/MPMI-18-0555Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene ExpressionSotirios E. TjamosEmmanouil FlemetakisEpaminondas J. PaplomatasPanagiotis KatinakisThe biocontrol bacterium Paenibacillus alvei K165 has the ability to protect Arabidopsis thaliana against Verticillium dahliae. A direct antagonistic action of strain K165 against V. dahliae was ruled out, making it likely that K165-mediated protection results from induced systemic resistance (ISR) in the host. K165-mediated protection was tested in various Arabidopsis mutants and transgenic plants impaired in defense signaling pathways, including NahG (transgenic line degrading salicylic acid [SA]), etr1-1 (insensitive to ethylene), jar1-1 (insensitive to jasmonate), npr1-1 (non-expressing NPR1 protein), pad3-1 (phytoalexin deficient), pad4-1 (phytoalexin deficient), eds5/sid1 (enhanced disease susceptibility), and sid2 (SA-induction deficient). ISR was blocked in blocked in Arabidopsis mutants npr1-1, eds5/sid1, and sid2, indicating that components of the pathway from isochorismate and a functional NPR1 play a crucial role in the K165-mediated ISR. Furthermore, the concomitant activation and increased transient accumulation of the PR-1, PR-2, and PR-5 genes were observed in the treatment in which both the inducing bacterial strain and the challenging pathogen were present in the rhizosphere of the A. thaliana plants.https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0555vascular wiltplant-growth-promoting rhizobacterium |
| spellingShingle | Sotirios E. Tjamos Emmanouil Flemetakis Epaminondas J. Paplomatas Panagiotis Katinakis Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression Molecular Plant-Microbe Interactions vascular wilt plant-growth-promoting rhizobacterium |
| title | Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression |
| title_full | Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression |
| title_fullStr | Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression |
| title_full_unstemmed | Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression |
| title_short | Induction of Resistance to Verticillium dahliae in Arabidopsis thaliana by the Biocontrol Agent K-165 and Pathogenesis-Related Proteins Gene Expression |
| title_sort | induction of resistance to verticillium dahliae in arabidopsis thaliana by the biocontrol agent k 165 and pathogenesis related proteins gene expression |
| topic | vascular wilt plant-growth-promoting rhizobacterium |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0555 |
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