MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion m...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | Journal of Cardiothoracic Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13019-021-01698-2 |
| _version_ | 1818826731842174976 |
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| author | Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou |
| author_facet | Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou |
| author_sort | Jiangwei Hu |
| collection | DOAJ |
| description | Abstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC. |
| first_indexed | 2024-12-19T00:32:20Z |
| format | Article |
| id | doaj.art-bcfa7ae1c27d4eefb1ad10a7f08c10d4 |
| institution | Directory Open Access Journal |
| issn | 1749-8090 |
| language | English |
| last_indexed | 2024-12-19T00:32:20Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cardiothoracic Surgery |
| spelling | doaj.art-bcfa7ae1c27d4eefb1ad10a7f08c10d42022-12-21T20:45:03ZengBMCJournal of Cardiothoracic Surgery1749-80902021-11-011611810.1186/s13019-021-01698-2MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progressionJiangwei Hu0Xinqin Xiang1Wei Guan2Weihua Lou3Junming He4Jian Chen5Yin Fu6Guoliang Lou7Department of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalDepartment of Cardiovascular Surgery, Yiwu Central HospitalAbstract Background So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). Methods MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. Results Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. Conclusion By targeting CDCA4, miR-497-5p restrained development of LUSC.https://doi.org/10.1186/s13019-021-01698-2miR-497-5pCDCA4LUSCProliferationMigrationInvasion |
| spellingShingle | Jiangwei Hu Xinqin Xiang Wei Guan Weihua Lou Junming He Jian Chen Yin Fu Guoliang Lou MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression Journal of Cardiothoracic Surgery miR-497-5p CDCA4 LUSC Proliferation Migration Invasion |
| title | MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression |
| title_full | MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression |
| title_fullStr | MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression |
| title_full_unstemmed | MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression |
| title_short | MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression |
| title_sort | mir 497 5p down regulates cdca4 to restrains lung squamous cell carcinoma progression |
| topic | miR-497-5p CDCA4 LUSC Proliferation Migration Invasion |
| url | https://doi.org/10.1186/s13019-021-01698-2 |
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