Arbutin improves gut development and serum lipids via Lactobacillus intestinalis
Arbutin has been widely studied in whitening, anti-inflammatory, and antioxidant. However, the interaction between arbutin and intestinal microbes has been rarely studied. Thus, mice were treated with arbutin concentrations of 0, 0.1, 0.2, 0.4, and 1 mg/ml. We found that arbutin promoted gut develop...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.948573/full |
| _version_ | 1798003378305892352 |
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| author | Jie Ma Shuai Chen Yuying Li Xin Wu Zehe Song |
| author_facet | Jie Ma Shuai Chen Yuying Li Xin Wu Zehe Song |
| author_sort | Jie Ma |
| collection | DOAJ |
| description | Arbutin has been widely studied in whitening, anti-inflammatory, and antioxidant. However, the interaction between arbutin and intestinal microbes has been rarely studied. Thus, mice were treated with arbutin concentrations of 0, 0.1, 0.2, 0.4, and 1 mg/ml. We found that arbutin promoted gut development such as villus length, villus areas, and villus length/crypt depth (L/D). Total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were significantly reduced by low concentrations of arbutin. Importantly, we analyzed the microbial composition in the control and 0.4 mg/ml arbutin group and found that the abundance of Lactobacillus intestinalis (L. intestinalis) was highest and enhanced in arbutin. Further, mice were fed with oral antibiotics and antibiotics + 0.4 mg/ml arbutin and then we transplanted fecal microbes from oral 0.4 mg/ml arbutin mice to mice pretreated with antibiotics. Our results showed that arbutin improves gut development, such as villus width, villus length, L/D, and villus areas. In addition, L. intestinalis monocolonization was carried out after a week of oral antibiotics and increased villus length, crypt depth, and villus areas. Finally, in vitro arbutin and L. intestinalis co-culture showed that arbutin promoted the growth and proliferation of L. intestinalis. Taken together, our results suggest that arbutin improves gut development and health of L. intestinalis. Future studies are needed to explore the function and mechanism of L. intestinalis affecting gut development. |
| first_indexed | 2024-04-11T12:06:28Z |
| format | Article |
| id | doaj.art-bcfdedf90022478a9981115f47b8d020 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T12:06:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-bcfdedf90022478a9981115f47b8d0202022-12-22T04:24:42ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.948573948573Arbutin improves gut development and serum lipids via Lactobacillus intestinalisJie Ma0Shuai Chen1Yuying Li2Xin Wu3Zehe Song4Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaKey Laboratory of Systems Health Science of Zhejiang Province, School of Life Sciences, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaArbutin has been widely studied in whitening, anti-inflammatory, and antioxidant. However, the interaction between arbutin and intestinal microbes has been rarely studied. Thus, mice were treated with arbutin concentrations of 0, 0.1, 0.2, 0.4, and 1 mg/ml. We found that arbutin promoted gut development such as villus length, villus areas, and villus length/crypt depth (L/D). Total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were significantly reduced by low concentrations of arbutin. Importantly, we analyzed the microbial composition in the control and 0.4 mg/ml arbutin group and found that the abundance of Lactobacillus intestinalis (L. intestinalis) was highest and enhanced in arbutin. Further, mice were fed with oral antibiotics and antibiotics + 0.4 mg/ml arbutin and then we transplanted fecal microbes from oral 0.4 mg/ml arbutin mice to mice pretreated with antibiotics. Our results showed that arbutin improves gut development, such as villus width, villus length, L/D, and villus areas. In addition, L. intestinalis monocolonization was carried out after a week of oral antibiotics and increased villus length, crypt depth, and villus areas. Finally, in vitro arbutin and L. intestinalis co-culture showed that arbutin promoted the growth and proliferation of L. intestinalis. Taken together, our results suggest that arbutin improves gut development and health of L. intestinalis. Future studies are needed to explore the function and mechanism of L. intestinalis affecting gut development.https://www.frontiersin.org/articles/10.3389/fnut.2022.948573/fullarbutingut developmentgut microbiotafecal microflora transplantationLactobacillus intestinalis |
| spellingShingle | Jie Ma Shuai Chen Yuying Li Xin Wu Zehe Song Arbutin improves gut development and serum lipids via Lactobacillus intestinalis Frontiers in Nutrition arbutin gut development gut microbiota fecal microflora transplantation Lactobacillus intestinalis |
| title | Arbutin improves gut development and serum lipids via Lactobacillus intestinalis |
| title_full | Arbutin improves gut development and serum lipids via Lactobacillus intestinalis |
| title_fullStr | Arbutin improves gut development and serum lipids via Lactobacillus intestinalis |
| title_full_unstemmed | Arbutin improves gut development and serum lipids via Lactobacillus intestinalis |
| title_short | Arbutin improves gut development and serum lipids via Lactobacillus intestinalis |
| title_sort | arbutin improves gut development and serum lipids via lactobacillus intestinalis |
| topic | arbutin gut development gut microbiota fecal microflora transplantation Lactobacillus intestinalis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.948573/full |
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