Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows
AimThis study aimed to examine the effects of feeding or abdominal brushing on the release of the hormones oxytocin, ACTH and cortisol during milking in dairy cows.MethodsTwelve cows in early lactation were used (2 × 2 factorial experimental design), testing the effects of two types of sensory stimu...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.671702/full |
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| author | Ewa Wredle Kerstin Svennersten-Sjaunja Lene Munksgaard Mette S. Herskin Rupert M. Bruckmaier Kerstin Uvnäs-Moberg |
| author_facet | Ewa Wredle Kerstin Svennersten-Sjaunja Lene Munksgaard Mette S. Herskin Rupert M. Bruckmaier Kerstin Uvnäs-Moberg |
| author_sort | Ewa Wredle |
| collection | DOAJ |
| description | AimThis study aimed to examine the effects of feeding or abdominal brushing on the release of the hormones oxytocin, ACTH and cortisol during milking in dairy cows.MethodsTwelve cows in early lactation were used (2 × 2 factorial experimental design), testing the effects of two types of sensory stimulation during milking over a 3 day period; feeding concentrate or manual abdominal brushing (1 stroke/s). Blood samples for hormone analyses were collected at time at −15, −1, 0 (onset of cluster), every min for 8 min, at 10, 12, 14, 16, 30, and 60 min. Hormone levels were assayed and AUC was calculated.ResultsMilking was associated with an immediate and significant rise of oxytocin. When milking was combined with feeding, significantly higher levels of oxytocin were observed at 2 and 4 mins (p < 0.05). No effect of brushing on oxytocin levels was observed. Milking alone was associated with a significant rise of ACTH levels. Feeding in connection with milking reduced the immediate rise of ACTH levels (p < 0.05) and AUC (p < 0.02), whereas no effects of brushing were found. Milking caused a progressive rise of cortisol levels. Concomitant feeding did not influence cortisol levels, whereas brushing significantly decreased cortisol levels at 1, 5 and 14 mins after onset of milking (p < 0.05).ConclusionFeeding increases oxytocin release in response to milking and decreases ACTH levels. Abdominal brushing did not influence these variables, but decreased cortisol levels. These data demonstrate that activation of afferent vagal nerve fibres and of cutaneous sensory nerves originating from the abdominal skin in front of the udder influence milking related hormone release differently. |
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| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-17T13:39:43Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-bcfe1f74efd24492a4c00ff07f59ed0c2022-12-21T21:46:19ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-03-011610.3389/fnins.2022.671702671702Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy CowsEwa Wredle0Kerstin Svennersten-Sjaunja1Lene Munksgaard2Mette S. Herskin3Rupert M. Bruckmaier4Kerstin Uvnäs-Moberg5Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, SwedenDepartment of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, SwedenDepartment of Animal Science, AU-Foulum, Aarhus University, Tjele, DenmarkDepartment of Animal Science, AU-Foulum, Aarhus University, Tjele, DenmarkVeterinary Physiology Vetsuisse Faculty, University of Bern, Bern, SwitzerlandDepartment of Animal Environment and Health, Swedish University of Agricultural Sciences, Skara, SwedenAimThis study aimed to examine the effects of feeding or abdominal brushing on the release of the hormones oxytocin, ACTH and cortisol during milking in dairy cows.MethodsTwelve cows in early lactation were used (2 × 2 factorial experimental design), testing the effects of two types of sensory stimulation during milking over a 3 day period; feeding concentrate or manual abdominal brushing (1 stroke/s). Blood samples for hormone analyses were collected at time at −15, −1, 0 (onset of cluster), every min for 8 min, at 10, 12, 14, 16, 30, and 60 min. Hormone levels were assayed and AUC was calculated.ResultsMilking was associated with an immediate and significant rise of oxytocin. When milking was combined with feeding, significantly higher levels of oxytocin were observed at 2 and 4 mins (p < 0.05). No effect of brushing on oxytocin levels was observed. Milking alone was associated with a significant rise of ACTH levels. Feeding in connection with milking reduced the immediate rise of ACTH levels (p < 0.05) and AUC (p < 0.02), whereas no effects of brushing were found. Milking caused a progressive rise of cortisol levels. Concomitant feeding did not influence cortisol levels, whereas brushing significantly decreased cortisol levels at 1, 5 and 14 mins after onset of milking (p < 0.05).ConclusionFeeding increases oxytocin release in response to milking and decreases ACTH levels. Abdominal brushing did not influence these variables, but decreased cortisol levels. These data demonstrate that activation of afferent vagal nerve fibres and of cutaneous sensory nerves originating from the abdominal skin in front of the udder influence milking related hormone release differently.https://www.frontiersin.org/articles/10.3389/fnins.2022.671702/fullcutaneous sensory nervesafferent vagal nervesfeedingbrushingoxytocindairy cows |
| spellingShingle | Ewa Wredle Kerstin Svennersten-Sjaunja Lene Munksgaard Mette S. Herskin Rupert M. Bruckmaier Kerstin Uvnäs-Moberg Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows Frontiers in Neuroscience cutaneous sensory nerves afferent vagal nerves feeding brushing oxytocin dairy cows |
| title | Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows |
| title_full | Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows |
| title_fullStr | Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows |
| title_full_unstemmed | Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows |
| title_short | Feeding and Manual Brushing Influence the Release of Oxytocin, ACTH and Cortisol Differently During Milking in Dairy Cows |
| title_sort | feeding and manual brushing influence the release of oxytocin acth and cortisol differently during milking in dairy cows |
| topic | cutaneous sensory nerves afferent vagal nerves feeding brushing oxytocin dairy cows |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.671702/full |
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