Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy
Abstract Underwater adhesive proteins secreted by organisms greatly inspires the development of underwater glue. However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related recombinant proteins, it is believed that abundant common...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40856-z |
| _version_ | 1797558254690107392 |
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| author | Yongchun Liu Ke Li Juanhua Tian Aiting Gao Lihua Tian Hao Su Shuting Miao Fei Tao Hao Ren Qingmin Yang Jing Cao Peng Yang |
| author_facet | Yongchun Liu Ke Li Juanhua Tian Aiting Gao Lihua Tian Hao Su Shuting Miao Fei Tao Hao Ren Qingmin Yang Jing Cao Peng Yang |
| author_sort | Yongchun Liu |
| collection | DOAJ |
| description | Abstract Underwater adhesive proteins secreted by organisms greatly inspires the development of underwater glue. However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related recombinant proteins, it is believed that abundant common proteins cannot be converted into underwater glue. Here, we demonstrate that unfolded common proteins exhibit high affinity to surfaces and strong internal cohesion via amyloid-like aggregation in water. Using bovine serum albumin (BSA) as a model protein, we obtain a stable unfolded protein by cleaving the disulfide bonds and maintaining the unfolded state by means of stabilizing agents such as trifluoroethanol (TFE) and urea. The diffusion of stabilizing agents into water exposes the hydrophobic residues of an unfolded protein and initiates aggregation of the unfolded protein into a solid block. A robust and stable underwater glue can thus be prepared from tens of common proteins. This strategy deciphers a general code in common proteins to construct robust underwater glue from abundant biomass. |
| first_indexed | 2024-03-10T17:28:47Z |
| format | Article |
| id | doaj.art-bcfed394efd54595b451cb396adee67f |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:28:47Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bcfed394efd54595b451cb396adee67f2023-11-20T10:06:25ZengNature PortfolioNature Communications2041-17232023-08-0114111510.1038/s41467-023-40856-zSynthesis of robust underwater glues from common proteins via unfolding-aggregating strategyYongchun Liu0Ke Li1Juanhua Tian2Aiting Gao3Lihua Tian4Hao Su5Shuting Miao6Fei Tao7Hao Ren8Qingmin Yang9Jing Cao10Peng Yang11Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityXi’an Key Laboratory for Prevention and Treatment of Common Aging Diseases, Translational and Research Centre for Prevention and Therapy of Chronic Disease, Institute of Basic and Translational Medicine, Xi’an Medical UniversityDepartment of Urology, The Second Affiliated Hospital of Xi’an Jiaotong UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityKey Laboratory of Archaeological Exploration and Cultural Heritage Conservation Technology, Ministry of Education, Institute of Culture and Heritage, Northwestern Polytechnical UniversityKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal UniversityAbstract Underwater adhesive proteins secreted by organisms greatly inspires the development of underwater glue. However, except for specific proteins such as mussel adhesive protein, barnacle cement proteins, curli protein and their related recombinant proteins, it is believed that abundant common proteins cannot be converted into underwater glue. Here, we demonstrate that unfolded common proteins exhibit high affinity to surfaces and strong internal cohesion via amyloid-like aggregation in water. Using bovine serum albumin (BSA) as a model protein, we obtain a stable unfolded protein by cleaving the disulfide bonds and maintaining the unfolded state by means of stabilizing agents such as trifluoroethanol (TFE) and urea. The diffusion of stabilizing agents into water exposes the hydrophobic residues of an unfolded protein and initiates aggregation of the unfolded protein into a solid block. A robust and stable underwater glue can thus be prepared from tens of common proteins. This strategy deciphers a general code in common proteins to construct robust underwater glue from abundant biomass.https://doi.org/10.1038/s41467-023-40856-z |
| spellingShingle | Yongchun Liu Ke Li Juanhua Tian Aiting Gao Lihua Tian Hao Su Shuting Miao Fei Tao Hao Ren Qingmin Yang Jing Cao Peng Yang Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy Nature Communications |
| title | Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy |
| title_full | Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy |
| title_fullStr | Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy |
| title_full_unstemmed | Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy |
| title_short | Synthesis of robust underwater glues from common proteins via unfolding-aggregating strategy |
| title_sort | synthesis of robust underwater glues from common proteins via unfolding aggregating strategy |
| url | https://doi.org/10.1038/s41467-023-40856-z |
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