Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens
Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1243819/full |
| _version_ | 1827807208856879104 |
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| author | Jin Liu Jin Liu Shuangqi Wu Shuangqi Wu Honghao Zhao Honghao Zhao Chun Ye Chun Ye Shulin Fu Shulin Fu Yu Liu Yu Liu Ting Liu Ting Liu Yinsheng Qiu Yinsheng Qiu |
| author_facet | Jin Liu Jin Liu Shuangqi Wu Shuangqi Wu Honghao Zhao Honghao Zhao Chun Ye Chun Ye Shulin Fu Shulin Fu Yu Liu Yu Liu Ting Liu Ting Liu Yinsheng Qiu Yinsheng Qiu |
| author_sort | Jin Liu |
| collection | DOAJ |
| description | Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of C. perfringens CVCC2030, it also evaluated the in vivo therapeutic effect on NE. The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system (agrB, agrD) and genes of VirS/R two-component regulatory system (virS, virR). In vivo experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Besides, baicalin-aluminum alleviated the upregulation of C. perfringens and Escherichia coli and raised the relative abundance of Lactobacillus in the ileal digesta. This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens infection by inhibiting the virulence-associated traits and virulence genes expression. |
| first_indexed | 2024-03-11T21:54:05Z |
| format | Article |
| id | doaj.art-bd004098af474b8b8d4b3214a8f8cb61 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T21:54:05Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bd004098af474b8b8d4b3214a8f8cb612023-09-26T05:57:37ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-09-011310.3389/fcimb.2023.12438191243819Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringensJin Liu0Jin Liu1Shuangqi Wu2Shuangqi Wu3Honghao Zhao4Honghao Zhao5Chun Ye6Chun Ye7Shulin Fu8Shulin Fu9Yu Liu10Yu Liu11Ting Liu12Ting Liu13Yinsheng Qiu14Yinsheng Qiu15Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, ChinaHubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaClostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of C. perfringens CVCC2030, it also evaluated the in vivo therapeutic effect on NE. The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system (agrB, agrD) and genes of VirS/R two-component regulatory system (virS, virR). In vivo experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Besides, baicalin-aluminum alleviated the upregulation of C. perfringens and Escherichia coli and raised the relative abundance of Lactobacillus in the ileal digesta. This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens infection by inhibiting the virulence-associated traits and virulence genes expression.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1243819/fullClostridium perfringensbaicalin-aluminumnecrotic enteritisvirulence factorsbroiler chickens |
| spellingShingle | Jin Liu Jin Liu Shuangqi Wu Shuangqi Wu Honghao Zhao Honghao Zhao Chun Ye Chun Ye Shulin Fu Shulin Fu Yu Liu Yu Liu Ting Liu Ting Liu Yinsheng Qiu Yinsheng Qiu Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens Frontiers in Cellular and Infection Microbiology Clostridium perfringens baicalin-aluminum necrotic enteritis virulence factors broiler chickens |
| title | Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens |
| title_full | Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens |
| title_fullStr | Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens |
| title_full_unstemmed | Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens |
| title_short | Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens |
| title_sort | baicalin aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of clostridium perfringens |
| topic | Clostridium perfringens baicalin-aluminum necrotic enteritis virulence factors broiler chickens |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1243819/full |
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