Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging
To systematically analyze biological changes with spatiotemporal dynamics, it is important to establish a system that is amenable for real-time in vivo imaging at various size levels. Herein, we focused on the developing pupal wing tissues in the pale grass blue butterfly, <i>Zizeeria maha<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Journal of Imaging |
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| Online Access: | https://www.mdpi.com/2313-433X/5/4/42 |
| _version_ | 1818497167126429696 |
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| author | Kanako Hirata Joji M. Otaki |
| author_facet | Kanako Hirata Joji M. Otaki |
| author_sort | Kanako Hirata |
| collection | DOAJ |
| description | To systematically analyze biological changes with spatiotemporal dynamics, it is important to establish a system that is amenable for real-time in vivo imaging at various size levels. Herein, we focused on the developing pupal wing tissues in the pale grass blue butterfly, <i>Zizeeria maha</i>, as a system of choice for a systematic multiscale approach in vivo in real time. We showed that the entire pupal wing could be monitored throughout development using a high-resolution bright-field time-lapse imaging system under the forewing-lift configuration; we recorded detailed dynamics of the dorsal and ventral epithelia that behaved independently for peripheral adjustment. We also monitored changes in the dorsal hindwing at the compartmental level and directly observed evaginating scale buds. We also employed a confocal laser microscopy system with multiple fluorescent dyes for three-dimensional observations at the tissue and cellular levels. We discovered extensive cellular clusters that may be functionally important as a unit of cellular communication and differentiation. We also identified epithelial discal and marginal dents that may function during development. Together, this lycaenid forewing system established a foundation to study the differentiation process of epithelial cells and can be used to study biophysically challenging mechanisms such as the determination of color patterns and scale nanoarchitecture at the multiscale levels. |
| first_indexed | 2024-12-10T18:42:16Z |
| format | Article |
| id | doaj.art-bd01a61b47224dfd92ac10d40c5484ff |
| institution | Directory Open Access Journal |
| issn | 2313-433X |
| language | English |
| last_indexed | 2024-12-10T18:42:16Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Imaging |
| spelling | doaj.art-bd01a61b47224dfd92ac10d40c5484ff2022-12-22T01:37:37ZengMDPI AGJournal of Imaging2313-433X2019-03-01544210.3390/jimaging5040042jimaging5040042Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale BioimagingKanako Hirata0Joji M. Otaki1The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, JapanThe BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, JapanTo systematically analyze biological changes with spatiotemporal dynamics, it is important to establish a system that is amenable for real-time in vivo imaging at various size levels. Herein, we focused on the developing pupal wing tissues in the pale grass blue butterfly, <i>Zizeeria maha</i>, as a system of choice for a systematic multiscale approach in vivo in real time. We showed that the entire pupal wing could be monitored throughout development using a high-resolution bright-field time-lapse imaging system under the forewing-lift configuration; we recorded detailed dynamics of the dorsal and ventral epithelia that behaved independently for peripheral adjustment. We also monitored changes in the dorsal hindwing at the compartmental level and directly observed evaginating scale buds. We also employed a confocal laser microscopy system with multiple fluorescent dyes for three-dimensional observations at the tissue and cellular levels. We discovered extensive cellular clusters that may be functionally important as a unit of cellular communication and differentiation. We also identified epithelial discal and marginal dents that may function during development. Together, this lycaenid forewing system established a foundation to study the differentiation process of epithelial cells and can be used to study biophysically challenging mechanisms such as the determination of color patterns and scale nanoarchitecture at the multiscale levels.https://www.mdpi.com/2313-433X/5/4/42butterfly wingcolor pattern formationforewing-lift methodlycaenid butterflypupal wing epitheliumreal-time in vivo imagingscale formationwhole-wing imaging<i>Zizeeria maha</i> |
| spellingShingle | Kanako Hirata Joji M. Otaki Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging Journal of Imaging butterfly wing color pattern formation forewing-lift method lycaenid butterfly pupal wing epithelium real-time in vivo imaging scale formation whole-wing imaging <i>Zizeeria maha</i> |
| title | Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging |
| title_full | Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging |
| title_fullStr | Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging |
| title_full_unstemmed | Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging |
| title_short | Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly <i>Zizeeria maha</i>: Establishing the Lycaenid System for Multiscale Bioimaging |
| title_sort | real time in vivo imaging of the developing pupal wing tissues in the pale grass blue butterfly i zizeeria maha i establishing the lycaenid system for multiscale bioimaging |
| topic | butterfly wing color pattern formation forewing-lift method lycaenid butterfly pupal wing epithelium real-time in vivo imaging scale formation whole-wing imaging <i>Zizeeria maha</i> |
| url | https://www.mdpi.com/2313-433X/5/4/42 |
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