Efek kandungan serat beras analog terhadap ekspresi GLUT4 otot rangka tikus diabetes
Effect of analog rice’s fiber on skeletal muscles GLUT4 expression in diabetic rats Background: Disruption of glucose transportation in skeletal muscle through GLUT4 becomes a problem in diabetes. Analog rice that had been modified by adding dietary fiber could improve the expression of GLUT4. Ob...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Indonesian |
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Universitas Gadjah Mada
2019-07-01
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| Series: | Jurnal Gizi Klinik Indonesia |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/jgki/article/view/31806 |
| Summary: | Effect of analog rice’s fiber on skeletal muscles GLUT4 expression in diabetic rats
Background: Disruption of glucose transportation in skeletal muscle through GLUT4 becomes a problem in diabetes. Analog rice that had been modified by adding dietary fiber could improve the expression of GLUT4.
Objective: This study aims to know the effect of dietary fiber toward GLUT4 expression and to know the dietary fiber percentage in analog rice.
Method: The research type is true experimental with post-test only group design. The samples consist of 24 male Wistar rats that are group into 4 groups (n=6 each group). Three groups were induced by giving a high-fat diet for 40 days and streptozotocin (STZ) 35 mg/kg BW was given at 33th day and one group was not induced. After the blood glucose level exceeded 135 mg/dl, the treatment was given. After 3 weeks, the rats were terminated and quadriceps femoris muscle tissue was taken for immunohistochemistry examination using rat GLUT4 polyclonal antibody. GLUT4 expression was quantified using an immunoreactive score (IRS-GLUT4). The data were analyzed using the Kruskal-Wallis test and Spearman test.
Results: Statistical analyses showed that there were significant differences between groups with a moderate positive correlation (correlation coefficient=0,651; p=0,003).
Conclusion: Dietary fiber in analog rice could improve skeletal muscle GLUT4 expression in Wistar rat diabetic model. |
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| ISSN: | 1693-900X 2502-4140 |