In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation
A biomaterial can replace the function of a real organ, conferring properties of support, regeneration or resistance. In the present investigation, a new composite was developed in the form of a polymeric membrane embedded with hydroxyapatite and alumina particles to be used as scaffolding and to al...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Results in Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590048X20300339 |
| _version_ | 1818211715984130048 |
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| author | Ana Karen Monrreal-Rodríguez Jesús Alberto Garibay-Alvarado Claudia Lucía Vargas-Requena Simón Yobanny Reyes-López |
| author_facet | Ana Karen Monrreal-Rodríguez Jesús Alberto Garibay-Alvarado Claudia Lucía Vargas-Requena Simón Yobanny Reyes-López |
| author_sort | Ana Karen Monrreal-Rodríguez |
| collection | DOAJ |
| description | A biomaterial can replace the function of a real organ, conferring properties of support, regeneration or resistance. In the present investigation, a new composite was developed in the form of a polymeric membrane embedded with hydroxyapatite and alumina particles to be used as scaffolding and to allow cell viability. The support matrix is poly ε-caprolactone, which is a biodegradable polymer, hydroxyapatite is the ceramic that contributes to the improvement of osteoconductive and osteo-regenerative properties, while alumina provides the hardness to the composite for its viable application in the orthopedic industry. The morphology of the composite resulted in an interweaving of fibers with a diameter of 840 ± 230 nm. The composites were analyzed to the MTT cytotoxicity test, showing that none of the composites were toxic (p = 0.0001); where the PCL/HA/α-Al2O3 composite showed greater cellular viability with 238%, demonstrating its possible usefulness as orthopedic material, in filling fractures, or bone imperfections caused by physical damage. |
| first_indexed | 2024-12-12T05:36:55Z |
| format | Article |
| id | doaj.art-bd0b5a94affe4296b053cd55e12ab12c |
| institution | Directory Open Access Journal |
| issn | 2590-048X |
| language | English |
| last_indexed | 2024-12-12T05:36:55Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Materials |
| spelling | doaj.art-bd0b5a94affe4296b053cd55e12ab12c2022-12-22T00:36:07ZengElsevierResults in Materials2590-048X2020-06-016100091In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferationAna Karen Monrreal-Rodríguez0Jesús Alberto Garibay-Alvarado1Claudia Lucía Vargas-Requena2Simón Yobanny Reyes-López3Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Envolvente Del PRONAF y Estocolmo S/n, Ciudad Juárez, Chih, C.P. 2300, MexicoInstituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Envolvente Del PRONAF y Estocolmo S/n, Ciudad Juárez, Chih, C.P. 2300, MexicoInstituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Envolvente Del PRONAF y Estocolmo S/n, Ciudad Juárez, Chih, C.P. 2300, MexicoCorresponding author.; Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Envolvente Del PRONAF y Estocolmo S/n, Ciudad Juárez, Chih, C.P. 2300, MexicoA biomaterial can replace the function of a real organ, conferring properties of support, regeneration or resistance. In the present investigation, a new composite was developed in the form of a polymeric membrane embedded with hydroxyapatite and alumina particles to be used as scaffolding and to allow cell viability. The support matrix is poly ε-caprolactone, which is a biodegradable polymer, hydroxyapatite is the ceramic that contributes to the improvement of osteoconductive and osteo-regenerative properties, while alumina provides the hardness to the composite for its viable application in the orthopedic industry. The morphology of the composite resulted in an interweaving of fibers with a diameter of 840 ± 230 nm. The composites were analyzed to the MTT cytotoxicity test, showing that none of the composites were toxic (p = 0.0001); where the PCL/HA/α-Al2O3 composite showed greater cellular viability with 238%, demonstrating its possible usefulness as orthopedic material, in filling fractures, or bone imperfections caused by physical damage.http://www.sciencedirect.com/science/article/pii/S2590048X20300339Cell viabilityPoly ε-caprolactoneHydroxyapatiteAlumina |
| spellingShingle | Ana Karen Monrreal-Rodríguez Jesús Alberto Garibay-Alvarado Claudia Lucía Vargas-Requena Simón Yobanny Reyes-López In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation Results in Materials Cell viability Poly ε-caprolactone Hydroxyapatite Alumina |
| title | In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation |
| title_full | In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation |
| title_fullStr | In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation |
| title_full_unstemmed | In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation |
| title_short | In vitro evaluation of poly-ε-caprolactone-hydroxypatite-alumina electrospun fibers on the fibroblast’s proliferation |
| title_sort | in vitro evaluation of poly ε caprolactone hydroxypatite alumina electrospun fibers on the fibroblast s proliferation |
| topic | Cell viability Poly ε-caprolactone Hydroxyapatite Alumina |
| url | http://www.sciencedirect.com/science/article/pii/S2590048X20300339 |
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