Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting

Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting

Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HR...

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Detalhes bibliográficos
Main Authors: Joas Lucas da Silva, Gabriela Guimaraes Sousa Leite, Gisele Medeiros Bastos, Beatriz Cacciacarro Lucas, Daniel Keniti Shinohara, Joice Sayuri Takinami, Marcelo Miyata, Cristina Moreno Fajardo, André Ducati Luchessi, Clarice Queico Fujimura Leite, Rosilene Fressatti Cardoso, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata
Formato: Artigo
Idioma:English
Publicado em: Fundação Oswaldo Cruz (FIOCRUZ) 2013-02-01
Colecção:Memorias do Instituto Oswaldo Cruz
Assuntos:
drug resistance
rifampicin
Mycobacterium tuberculosis
Acesso em linha:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100017&lng=en&tlng=en

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