Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication
Tobacco mosaic virus (TMV) elicits a hypersensitive response (HR) in tobacco plants that carry the N gene. To identify the elicitor of this HR, Agrobacteriumn tumefaciens was used as a vector for the transient expression of TMV replicase proteins, movement protein, and coat protein in NN and nn toba...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1998-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1242 |
| _version_ | 1811264535772790784 |
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| author | Truus E. M. Abbink Pernilla A. Tjernberg John F. Bol Huub J. M. Linthorst |
| author_facet | Truus E. M. Abbink Pernilla A. Tjernberg John F. Bol Huub J. M. Linthorst |
| author_sort | Truus E. M. Abbink |
| collection | DOAJ |
| description | Tobacco mosaic virus (TMV) elicits a hypersensitive response (HR) in tobacco plants that carry the N gene. To identify the elicitor of this HR, Agrobacteriumn tumefaciens was used as a vector for the transient expression of TMV replicase proteins, movement protein, and coat protein in NN and nn tobacco. Transient expression of the 126K protein and fragments thereof containing the helicase motifs induced necrosis and systemic expression of the pathogenesis-related PR-1a gene in NN plants but not in nnplants. The results confirm previous evidence that the TMV helicase sequence is the elicitor of the HR (H. S. Padgett, Y. Watanabe, and R. N. Beachy, Mol. Plant-Microbe Interact. 10:709-715, 1997) and demonstrate that this helicase sequence acts as an elicitor of HR in the absence of other viral proteins or RNA replication. |
| first_indexed | 2024-04-12T20:06:05Z |
| format | Article |
| id | doaj.art-bd147489c0784f07a6905638f8976496 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:05Z |
| publishDate | 1998-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-bd147489c0784f07a6905638f89764962022-12-22T03:18:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-12-0111121242124610.1094/MPMI.1998.11.12.1242Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus ReplicationTruus E. M. AbbinkPernilla A. TjernbergJohn F. BolHuub J. M. LinthorstTobacco mosaic virus (TMV) elicits a hypersensitive response (HR) in tobacco plants that carry the N gene. To identify the elicitor of this HR, Agrobacteriumn tumefaciens was used as a vector for the transient expression of TMV replicase proteins, movement protein, and coat protein in NN and nn tobacco. Transient expression of the 126K protein and fragments thereof containing the helicase motifs induced necrosis and systemic expression of the pathogenesis-related PR-1a gene in NN plants but not in nnplants. The results confirm previous evidence that the TMV helicase sequence is the elicitor of the HR (H. S. Padgett, Y. Watanabe, and R. N. Beachy, Mol. Plant-Microbe Interact. 10:709-715, 1997) and demonstrate that this helicase sequence acts as an elicitor of HR in the absence of other viral proteins or RNA replication.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1242 |
| spellingShingle | Truus E. M. Abbink Pernilla A. Tjernberg John F. Bol Huub J. M. Linthorst Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication Molecular Plant-Microbe Interactions |
| title | Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication |
| title_full | Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication |
| title_fullStr | Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication |
| title_full_unstemmed | Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication |
| title_short | Tobacco Mosaic Virus Helicase Domain Induces Necrosis in N Gene-Carrying Tobacco in the Absence of Virus Replication |
| title_sort | tobacco mosaic virus helicase domain induces necrosis in n gene carrying tobacco in the absence of virus replication |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1242 |
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