Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

Abstract Background Mucosal vaccines have the potential to induce protective immune responses at the sites of infection. Applying CRISPR/Cas9 editing, we aimed to develop a probiotic-based vaccine candidate expressing the HIV-1 envelope membrane-proximal external region (MPER) on the surface of E. c...

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Bibliographic Details
Main Authors: Nathaniel Ninyio, Katharina Schmitt, Gladys Sergon, Charlotta Nilsson, Sören Andersson, Nikolai Scherbak
Format: Article
Language:English
Published: BMC 2024-02-01
Series:Microbial Cell Factories
Subjects:
CRISPR/Cas9
HIV-1
Probiotic
Membrane-proximal external region (MPER)
Outer membrane protein F (OmpF)
Online Access:https://doi.org/10.1186/s12934-023-02290-0

Internet

https://doi.org/10.1186/s12934-023-02290-0

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