Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish
Abstract Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated mic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-023-00311-1 |
| _version_ | 1827633564553838592 |
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| author | Daehan Kim Que Thanh Thanh Nguyen Seungjin Lee Kyung-Mi Choi Eun-Ju Lee Joong Yull Park |
| author_facet | Daehan Kim Que Thanh Thanh Nguyen Seungjin Lee Kyung-Mi Choi Eun-Ju Lee Joong Yull Park |
| author_sort | Daehan Kim |
| collection | DOAJ |
| description | Abstract Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment. |
| first_indexed | 2024-03-09T15:01:18Z |
| format | Article |
| id | doaj.art-bd14e085655d4586a66fe1139df02753 |
| institution | Directory Open Access Journal |
| issn | 2373-8065 |
| language | English |
| last_indexed | 2024-03-09T15:01:18Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj.art-bd14e085655d4586a66fe1139df027532023-11-26T13:53:54ZengNature Portfolionpj Microgravity2373-80652023-08-019111110.1038/s41526-023-00311-1Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dishDaehan Kim0Que Thanh Thanh Nguyen1Seungjin Lee2Kyung-Mi Choi3Eun-Ju Lee4Joong Yull Park5Department of Mechanical Engineering, Graduate School, Chung-Ang UniversityDepartment of Obstetrics and Gynecology, School of Medicine, Chung-Ang UniversityDepartment of Mechanical Engineering, Graduate School, Chung-Ang UniversityDepartment of Obstetrics and Gynecology, School of Medicine, Chung-Ang UniversityDepartment of Obstetrics and Gynecology, School of Medicine, Chung-Ang UniversityDepartment of Mechanical Engineering, Graduate School, Chung-Ang UniversityAbstract Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment.https://doi.org/10.1038/s41526-023-00311-1 |
| spellingShingle | Daehan Kim Que Thanh Thanh Nguyen Seungjin Lee Kyung-Mi Choi Eun-Ju Lee Joong Yull Park Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish npj Microgravity |
| title | Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish |
| title_full | Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish |
| title_fullStr | Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish |
| title_full_unstemmed | Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish |
| title_short | Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish |
| title_sort | customized small sized clinostat using 3d printing and gas permeable polydimethylsiloxane culture dish |
| url | https://doi.org/10.1038/s41526-023-00311-1 |
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