<i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats
Dried tangerine peel (<i>Citri reticulatae Pericarpium</i>, CRP; Chenpi in Chinese) possesses medicine and food homology with hypolipidemic, anti-inflammatory and antioxidant activities. This study aimed to explore the protective effect of CRP extract on endothelial function and inflamma...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/24/5221 |
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| author | Yuehan Wang Xutao Zhang Chunxiu Zhou Haroon Khan Manqin Fu Wai San Cheang |
| author_facet | Yuehan Wang Xutao Zhang Chunxiu Zhou Haroon Khan Manqin Fu Wai San Cheang |
| author_sort | Yuehan Wang |
| collection | DOAJ |
| description | Dried tangerine peel (<i>Citri reticulatae Pericarpium</i>, CRP; Chenpi in Chinese) possesses medicine and food homology with hypolipidemic, anti-inflammatory and antioxidant activities. This study aimed to explore the protective effect of CRP extract on endothelial function and inflammation in type 2 diabetic rats and the related mechanisms. Type 2 diabetes mellitus was induced by high-fat diet (HFD)/streptozotocin (STZ) in male Sprague Dawley rats, and CRP extract was orally administered at 400 mg/kg/day for 4 weeks. Rat and mouse aortas were treated with high glucose and CRP extract ex vivo. The data showed that the ethanolic extract of CRP normalized blood pressure and the plasma lipid profile as well as the plasma levels of liver enzymes in diabetic rats. Impaired endothelium-dependent relaxations in aortas, carotid arteries and renal arteries were improved. CRP extract suppressed vascular inflammatory markers and induced AMPK activation in aortas of diabetic rats. Exposure to high glucose impaired vasodilation in aortas of rats and mice, and this impairment was prevented by co-incubation with CRP extract. In conclusion, our findings suggest that CRP extract protects endothelial function by inhibiting the vascular inflammatory state on activation of AMPK in diabetic rats. |
| first_indexed | 2024-03-09T16:00:38Z |
| format | Article |
| id | doaj.art-bd170fa923e14aa89b201b95f45d589d |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T16:00:38Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-bd170fa923e14aa89b201b95f45d589d2023-11-24T17:07:34ZengMDPI AGNutrients2072-66432022-12-011424522110.3390/nu14245221<i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic RatsYuehan Wang0Xutao Zhang1Chunxiu Zhou2Haroon Khan3Manqin Fu4Wai San Cheang5Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, ChinaInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, ChinaInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, ChinaDepartment of Pharmacy, Abdul Wali Khan University Mardan, Mardan 23200, PakistanSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR 999078, ChinaDried tangerine peel (<i>Citri reticulatae Pericarpium</i>, CRP; Chenpi in Chinese) possesses medicine and food homology with hypolipidemic, anti-inflammatory and antioxidant activities. This study aimed to explore the protective effect of CRP extract on endothelial function and inflammation in type 2 diabetic rats and the related mechanisms. Type 2 diabetes mellitus was induced by high-fat diet (HFD)/streptozotocin (STZ) in male Sprague Dawley rats, and CRP extract was orally administered at 400 mg/kg/day for 4 weeks. Rat and mouse aortas were treated with high glucose and CRP extract ex vivo. The data showed that the ethanolic extract of CRP normalized blood pressure and the plasma lipid profile as well as the plasma levels of liver enzymes in diabetic rats. Impaired endothelium-dependent relaxations in aortas, carotid arteries and renal arteries were improved. CRP extract suppressed vascular inflammatory markers and induced AMPK activation in aortas of diabetic rats. Exposure to high glucose impaired vasodilation in aortas of rats and mice, and this impairment was prevented by co-incubation with CRP extract. In conclusion, our findings suggest that CRP extract protects endothelial function by inhibiting the vascular inflammatory state on activation of AMPK in diabetic rats.https://www.mdpi.com/2072-6643/14/24/5221tangerine peeldiabetes mellitusendothelial dysfunctioninflammationAMPK |
| spellingShingle | Yuehan Wang Xutao Zhang Chunxiu Zhou Haroon Khan Manqin Fu Wai San Cheang <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats Nutrients tangerine peel diabetes mellitus endothelial dysfunction inflammation AMPK |
| title | <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats |
| title_full | <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats |
| title_fullStr | <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats |
| title_full_unstemmed | <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats |
| title_short | <i>Citri Reticulatae Pericarpium</i> (Chenpi) Protects against Endothelial Dysfunction and Vascular Inflammation in Diabetic Rats |
| title_sort | i citri reticulatae pericarpium i chenpi protects against endothelial dysfunction and vascular inflammation in diabetic rats |
| topic | tangerine peel diabetes mellitus endothelial dysfunction inflammation AMPK |
| url | https://www.mdpi.com/2072-6643/14/24/5221 |
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