Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance
Background: Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Met...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020312998 |
| _version_ | 1811292253670342656 |
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| author | Carolina Simão Albano Anderson Moreira Gomes Geórgia da Silva Feltran Célio Junior da Costa Fernandes Luciana Daniele Trino Willian Fernando Zambuzzi Paulo Noronha Lisboa-Filho |
| author_facet | Carolina Simão Albano Anderson Moreira Gomes Geórgia da Silva Feltran Célio Junior da Costa Fernandes Luciana Daniele Trino Willian Fernando Zambuzzi Paulo Noronha Lisboa-Filho |
| author_sort | Carolina Simão Albano |
| collection | DOAJ |
| description | Background: Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Methods: The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. Results: Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-β1, Fak, and Src. These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp). Conclusion: Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field. |
| first_indexed | 2024-04-13T04:42:39Z |
| format | Article |
| id | doaj.art-bd1e9c1345ad465a813ff7b23e7945fb |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T04:42:39Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bd1e9c1345ad465a813ff7b23e7945fb2022-12-22T03:01:57ZengElsevierHeliyon2405-84402020-07-0167e04455Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performanceCarolina Simão Albano0Anderson Moreira Gomes1Geórgia da Silva Feltran2Célio Junior da Costa Fernandes3Luciana Daniele Trino4Willian Fernando Zambuzzi5Paulo Noronha Lisboa-Filho6Bioassays and Cell Dynamics Laboratory – UNESP – São Paulo State University, Biosciences Institute, Department of Chemistry and Biochemistry, Botucatu, Brazil; Advanced Materials and Nanotechnology Laboratory – UNESP – São Paulo State University School of Sciences, Department of Physics, Bauru, BrazilBioassays and Cell Dynamics Laboratory – UNESP – São Paulo State University, Biosciences Institute, Department of Chemistry and Biochemistry, Botucatu, BrazilBioassays and Cell Dynamics Laboratory – UNESP – São Paulo State University, Biosciences Institute, Department of Chemistry and Biochemistry, Botucatu, BrazilBioassays and Cell Dynamics Laboratory – UNESP – São Paulo State University, Biosciences Institute, Department of Chemistry and Biochemistry, Botucatu, BrazilAdvanced Materials and Nanotechnology Laboratory – UNESP – São Paulo State University School of Sciences, Department of Physics, Bauru, BrazilBioassays and Cell Dynamics Laboratory – UNESP – São Paulo State University, Biosciences Institute, Department of Chemistry and Biochemistry, Botucatu, BrazilAdvanced Materials and Nanotechnology Laboratory – UNESP – São Paulo State University School of Sciences, Department of Physics, Bauru, Brazil; Corresponding author.Background: Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Methods: The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. Results: Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-β1, Fak, and Src. These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp). Conclusion: Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field.http://www.sciencedirect.com/science/article/pii/S2405844020312998Materials scienceCell biologyImplantsBiocompatibilityTrace elementsOsseointegration |
| spellingShingle | Carolina Simão Albano Anderson Moreira Gomes Geórgia da Silva Feltran Célio Junior da Costa Fernandes Luciana Daniele Trino Willian Fernando Zambuzzi Paulo Noronha Lisboa-Filho Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance Heliyon Materials science Cell biology Implants Biocompatibility Trace elements Osseointegration |
| title | Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| title_full | Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| title_fullStr | Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| title_full_unstemmed | Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| title_short | Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| title_sort | biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance |
| topic | Materials science Cell biology Implants Biocompatibility Trace elements Osseointegration |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020312998 |
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