Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig
Deoxynivalenol (DON) is a mycotoxin that is found in feed ingredients derived from grains such as corn and wheat. Consumption of DON-contaminated feed has been shown to cause damage to the intestine, kidneys, and liver. However, the molecular mechanism by which DON exerts its effect in the small int...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/9/535 |
| _version_ | 1827656287046860800 |
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| author | Tae Hong Kang Kyung Soo Kang Sang In Lee |
| author_facet | Tae Hong Kang Kyung Soo Kang Sang In Lee |
| author_sort | Tae Hong Kang |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is a mycotoxin that is found in feed ingredients derived from grains such as corn and wheat. Consumption of DON-contaminated feed has been shown to cause damage to the intestine, kidneys, and liver. However, the molecular mechanism by which DON exerts its effect in the small intestine is not completely understood. As a result, we profiled gene expression in intestinal epithelial cells treated with DON and examined the molecular function in vitro. We hypothesized that DON could induce apoptosis via the FOXO3a-signaling pathway in intestinal epithelial cells based on these findings. DON induced the apoptosis and the translocation of <i>FOXO3a</i> into the nucleus. Moreover, the inhibiting of <i>FOXO3a</i> alleviated the apoptosis and expression of apoptosis-related genes (<i>TRAL</i>, <i>BCL-6</i>, <i>CASP8</i>, and <i>CASP3</i>). ERK1/2 inhibitor treatment suppressed the translocation of <i>FOXO3a</i> into the nucleus. Our discovery suggests that DON induces apoptosis in intestinal epithelial cells through the FOXO3a-signaling pathway. |
| first_indexed | 2024-03-09T22:20:28Z |
| format | Article |
| id | doaj.art-bd24a46c91024c72a531c6d4a91e63da |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T22:20:28Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-bd24a46c91024c72a531c6d4a91e63da2023-11-23T19:15:50ZengMDPI AGToxics2305-63042022-09-0110953510.3390/toxics10090535Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in PigTae Hong Kang0Kyung Soo Kang1Sang In Lee2Department of Animal Science and Biotechnology, Kyungpook National University, Sangju-si 37224, KoreaDepartment of Bio Life Sciences, Shingu College, Seongnam-si 13174, KoreaDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju-si 37224, KoreaDeoxynivalenol (DON) is a mycotoxin that is found in feed ingredients derived from grains such as corn and wheat. Consumption of DON-contaminated feed has been shown to cause damage to the intestine, kidneys, and liver. However, the molecular mechanism by which DON exerts its effect in the small intestine is not completely understood. As a result, we profiled gene expression in intestinal epithelial cells treated with DON and examined the molecular function in vitro. We hypothesized that DON could induce apoptosis via the FOXO3a-signaling pathway in intestinal epithelial cells based on these findings. DON induced the apoptosis and the translocation of <i>FOXO3a</i> into the nucleus. Moreover, the inhibiting of <i>FOXO3a</i> alleviated the apoptosis and expression of apoptosis-related genes (<i>TRAL</i>, <i>BCL-6</i>, <i>CASP8</i>, and <i>CASP3</i>). ERK1/2 inhibitor treatment suppressed the translocation of <i>FOXO3a</i> into the nucleus. Our discovery suggests that DON induces apoptosis in intestinal epithelial cells through the FOXO3a-signaling pathway.https://www.mdpi.com/2305-6304/10/9/535apoptosisdeoxynivalenoldifferentially expressed genesForkhead box |
| spellingShingle | Tae Hong Kang Kyung Soo Kang Sang In Lee Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig Toxics apoptosis deoxynivalenol differentially expressed genes Forkhead box |
| title | Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig |
| title_full | Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig |
| title_fullStr | Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig |
| title_full_unstemmed | Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig |
| title_short | Deoxynivalenol Induces Apoptosis via FOXO3a-Signaling Pathway in Small-Intestinal Cells in Pig |
| title_sort | deoxynivalenol induces apoptosis via foxo3a signaling pathway in small intestinal cells in pig |
| topic | apoptosis deoxynivalenol differentially expressed genes Forkhead box |
| url | https://www.mdpi.com/2305-6304/10/9/535 |
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