Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity
γ-Aminobutyric (GABA) acid is a nutrient and signaling molecule existing in many plants, participating in the regulation of metabolism and various physiological activities. Two strains of <i>Hypsizygus marmoreus</i> (a white variety and a brown variety) were investigated to study the imp...
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MDPI AG
2024-01-01
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| author | Zhi Cao Hongyu Chen Chenli Zhou Ming Gong Yan Li Youran Shao Yingying Wu Dapeng Bao |
| author_facet | Zhi Cao Hongyu Chen Chenli Zhou Ming Gong Yan Li Youran Shao Yingying Wu Dapeng Bao |
| author_sort | Zhi Cao |
| collection | DOAJ |
| description | γ-Aminobutyric (GABA) acid is a nutrient and signaling molecule existing in many plants, participating in the regulation of metabolism and various physiological activities. Two strains of <i>Hypsizygus marmoreus</i> (a white variety and a brown variety) were investigated to study the impact of exogenous GABA on mycelial growth and the response to stress. Mycelial growth, microscopic morphology, antioxidant profile, and <i>gad2</i> expression in <i>H. marmoreu</i> were investigated under salt, dehydration, or cold stress. The results indicated that 5 mM GABA stimulated mycelial growth under standard cultivation conditions, whereas GABA addition over 10 mM hindered the growth. Under salt, dehydration, or cold stress, treatment with 5 mM GABA significantly enhanced the mycelial growth rate and density of both <i>H. marmoreus</i> strains by promoting front hyphae branching. Meanwhile, the activities of key antioxidant enzymes such as peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were enhanced by GABA, thereby augmenting the defensive network against abiotic stress. Additionally, <i>gad2</i> expression and GABA concentration were increased under abiotic stresses as a resistance regulation response. The exogenous addition of GABA strengthened the upregulation of <i>gad2</i> expression and GABA production. These findings indicated that exogenously adding low concentrations of GABA effectively enhanced the mycelial growth and antioxidant profile of <i>H. marmoreus</i>, thereby improving its resistance against stresses. |
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| issn | 2218-1989 |
| language | English |
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| spelling | doaj.art-bd2513ba551446b188f503049e437c5f2024-02-23T15:27:00ZengMDPI AGMetabolites2218-19892024-01-011429410.3390/metabo14020094Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant CapacityZhi Cao0Hongyu Chen1Chenli Zhou2Ming Gong3Yan Li4Youran Shao5Yingying Wu6Dapeng Bao7School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaNational Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, Chinaγ-Aminobutyric (GABA) acid is a nutrient and signaling molecule existing in many plants, participating in the regulation of metabolism and various physiological activities. Two strains of <i>Hypsizygus marmoreus</i> (a white variety and a brown variety) were investigated to study the impact of exogenous GABA on mycelial growth and the response to stress. Mycelial growth, microscopic morphology, antioxidant profile, and <i>gad2</i> expression in <i>H. marmoreu</i> were investigated under salt, dehydration, or cold stress. The results indicated that 5 mM GABA stimulated mycelial growth under standard cultivation conditions, whereas GABA addition over 10 mM hindered the growth. Under salt, dehydration, or cold stress, treatment with 5 mM GABA significantly enhanced the mycelial growth rate and density of both <i>H. marmoreus</i> strains by promoting front hyphae branching. Meanwhile, the activities of key antioxidant enzymes such as peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were enhanced by GABA, thereby augmenting the defensive network against abiotic stress. Additionally, <i>gad2</i> expression and GABA concentration were increased under abiotic stresses as a resistance regulation response. The exogenous addition of GABA strengthened the upregulation of <i>gad2</i> expression and GABA production. These findings indicated that exogenously adding low concentrations of GABA effectively enhanced the mycelial growth and antioxidant profile of <i>H. marmoreus</i>, thereby improving its resistance against stresses.https://www.mdpi.com/2218-1989/14/2/94<i>Hypsizygus marmoreus</i>GABAexogenous additionmycelial growthantioxidant enzyme |
| spellingShingle | Zhi Cao Hongyu Chen Chenli Zhou Ming Gong Yan Li Youran Shao Yingying Wu Dapeng Bao Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity Metabolites <i>Hypsizygus marmoreus</i> GABA exogenous addition mycelial growth antioxidant enzyme |
| title | Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity |
| title_full | Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity |
| title_fullStr | Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity |
| title_full_unstemmed | Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity |
| title_short | Exogenous γ-Aminobutyric Acid (GABA) Enhanced Response to Abiotic Stress in <i>Hypsizygus marmoreus</i> by Improving Mycelial Growth and Antioxidant Capacity |
| title_sort | exogenous γ aminobutyric acid gaba enhanced response to abiotic stress in i hypsizygus marmoreus i by improving mycelial growth and antioxidant capacity |
| topic | <i>Hypsizygus marmoreus</i> GABA exogenous addition mycelial growth antioxidant enzyme |
| url | https://www.mdpi.com/2218-1989/14/2/94 |
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