Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro
Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.681131/full |
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| author | Kaila Petronila Medina-Alarcón Iara Pengo Tobias da Silva Giovana Garcia Ferin Marcelo A. Pereira-da-Silva Marcelo A. Pereira-da-Silva Caroline Maria Marcos Mariana Bastos dos Santos Luis Octávio Regasini Marlus Chorilli Maria José S. Mendes-Giannini Fernando Rogerio Pavan Ana Marisa Fusco-Almeida |
| author_facet | Kaila Petronila Medina-Alarcón Iara Pengo Tobias da Silva Giovana Garcia Ferin Marcelo A. Pereira-da-Silva Marcelo A. Pereira-da-Silva Caroline Maria Marcos Mariana Bastos dos Santos Luis Octávio Regasini Marlus Chorilli Maria José S. Mendes-Giannini Fernando Rogerio Pavan Ana Marisa Fusco-Almeida |
| author_sort | Kaila Petronila Medina-Alarcón |
| collection | DOAJ |
| description | Co-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities. |
| first_indexed | 2024-12-17T19:30:42Z |
| format | Article |
| id | doaj.art-bd26c32f3c854333a52fd09a4c0c7ff0 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-17T19:30:42Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bd26c32f3c854333a52fd09a4c0c7ff02022-12-21T21:35:15ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-11-011110.3389/fcimb.2021.681131681131Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In VitroKaila Petronila Medina-Alarcón0Iara Pengo Tobias da Silva1Giovana Garcia Ferin2Marcelo A. Pereira-da-Silva3Marcelo A. Pereira-da-Silva4Caroline Maria Marcos5Mariana Bastos dos Santos6Luis Octávio Regasini7Marlus Chorilli8Maria José S. Mendes-Giannini9Fernando Rogerio Pavan10Ana Marisa Fusco-Almeida11School of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilSchool of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilSchool of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilInstitute of Physics of Sao Carlos (IFSC)-University of Sao Paulo (USP) IFSC/USP, Sao Carlos, BrazilExact Sciences and Engineering, Paulista Central University Center (UNICEP), Säo Carlos, BrazilSchool of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilDepartment of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista, São José do Rio Preto, BrazilDepartment of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, Universidade Estadual Paulista, São José do Rio Preto, BrazilDepartment of Drug and Medicines, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Araraquara, BrazilSchool of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilDepartment of Biological, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Araraquara, BrazilSchool of Pharmaceutical Sciences, Department of Clinical Analysis, Universidade Estadual Paulista (UNESP), Araraquara, BrazilCo-infection of Mycobacterium tuberculosis and Paracoccidioides brasiliensis, present in 20% in Latin America, is a public health problem due to a lack of adequate diagnosis. These microorganisms are capable of forming biofilms, mainly in immunocompromised patients, which can lead to death due to the lack of effective treatment for both diseases. The present research aims to show for the first time the formation of mixed biofilms of M. tuberculosis and P. brasiliensis (Pb18) in vitro, as well as to evaluate the action of 3’hydroxychalcone (3’chalc) -loaded nanoemulsion (NE) (NE3’chalc) against monospecies and mixed biofilms, the formation of mixed biofilms of M. tuberculosis H37Rv (ATCC 27294), 40Rv (clinical strains) and P. brasiliensis (Pb18) (ATCC 32069), and the first condition of formation (H37Rv +Pb18) and (40Rv + Pb18) and second condition of formation (Pb18 + H37Rv) with 45 days of total formation time under both conditions. The results of mixed biofilms (H37Rv + Pb18) and (40Rv + Pb18), showed an organized network of M. tuberculosis bacilli in which P. brasiliensis yeasts are connected with a highly extracellular polysaccharide matrix. The (Pb18 + H37Rv) showed a dense biofilm with an apparent predominance of P. brasiliensis and fragments of M. tuberculosis. PCR assays confirmed the presence of the microorganisms involved in this formation. The characterization of NE and NE3’chalc displayed sizes from 145.00 ± 1.05 and 151.25 ± 0.60, a polydispersity index (PDI) from 0.20± 0.01 to 0.16± 0.01, and zeta potential -58.20 ± 0.92 mV and -56.10 ± 0.71 mV, respectively. The atomic force microscopy (AFM) results showed lamellar structures characteristic of NE. The minimum inhibitory concentration (MIC) values of 3’hidroxychalcone (3’chalc) range from 0.97- 7.8 µg/mL and NE3’chalc from 0.24 - 3.9 µg/mL improved the antibacterial activity when compared with 3’chalc-free, no cytotoxicity. Antibiofilm assays proved the efficacy of 3’chalc-free incorporation in NE. These findings contribute to a greater understanding of the formation of M. tuberculosis and P. brasiliensis in the mixed biofilm. In addition, the findings present a new possible NE3’chalc treatment alternative for the mixed biofilms of these microorganisms, with a high degree of relevance due to the lack of other treatments for these comorbidities.https://www.frontiersin.org/articles/10.3389/fcimb.2021.681131/fullmixed biofilmMycobacterium tuberculosisParacoccidioides brasiliensisnanoemulsion3’hydroxychalcone |
| spellingShingle | Kaila Petronila Medina-Alarcón Iara Pengo Tobias da Silva Giovana Garcia Ferin Marcelo A. Pereira-da-Silva Marcelo A. Pereira-da-Silva Caroline Maria Marcos Mariana Bastos dos Santos Luis Octávio Regasini Marlus Chorilli Maria José S. Mendes-Giannini Fernando Rogerio Pavan Ana Marisa Fusco-Almeida Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro Frontiers in Cellular and Infection Microbiology mixed biofilm Mycobacterium tuberculosis Paracoccidioides brasiliensis nanoemulsion 3’hydroxychalcone |
| title | Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
| title_full | Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
| title_fullStr | Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
| title_full_unstemmed | Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
| title_short | Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro |
| title_sort | mycobacterium tuberculosis and paracoccidioides brasiliensis formation and treatment of mixed biofilm in vitro |
| topic | mixed biofilm Mycobacterium tuberculosis Paracoccidioides brasiliensis nanoemulsion 3’hydroxychalcone |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.681131/full |
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