Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals
Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunct...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00072/full |
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| author | Rene Arredondo-Hernández Max Schmulson Patricia Orduña Gamaliel López-Leal Angel-Mario Zarate Gerardo Alanis-Funes Luis David Alcaraz Rubí Santiago-Cruz Miguel A. Cevallos Antonio R. Villa Samuel Ponce-de-León Rosales Yolanda López-Vidal Consorcio Mexicano para el Estudio del Microbioma Humano |
| author_facet | Rene Arredondo-Hernández Max Schmulson Patricia Orduña Gamaliel López-Leal Angel-Mario Zarate Gerardo Alanis-Funes Luis David Alcaraz Rubí Santiago-Cruz Miguel A. Cevallos Antonio R. Villa Samuel Ponce-de-León Rosales Yolanda López-Vidal Consorcio Mexicano para el Estudio del Microbioma Humano |
| author_sort | Rene Arredondo-Hernández |
| collection | DOAJ |
| description | Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunction with colonic mucosa dysbiosis, is necessary to determine IBS physiopathology and the effects of environmental pressures. Using a simple polygenic model, host single nucleotide polymorphisms (SNPs) and the taxonomic compositions of microbiota were compared between IBS patients and healthy subjects. As proof of concept, five IBS-Rome III patients and five healthy controls (HCs) were systematically studied. The human and bacterial intestinal metagenome of each subject was taxonomically annotated and screened for previously annotated IBS, ulcerative colitis, and Crohn's disease-associated SNPs or taxon abundance. Dietary data and fecal markers were collected and associated with the intestinal microbiome. However, more than 1,000 variants were found, and at least 76 SNPs differentiated IBS patients from HCs, as did associations with 4 phyla and 10 bacterial genera. In this study, we found elements supporting a polygenic background, with frequent variants, among the Mestizo population, and the colonic mucosal enrichment of Bacteroides, Alteromonas, Neisseria, Streptococcus, and Microbacterium, may serve as a hallmark for IBS. |
| first_indexed | 2024-12-18T23:11:35Z |
| format | Article |
| id | doaj.art-bd275a09a2334e3086b12792995fc420 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-18T23:11:35Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-bd275a09a2334e3086b12792995fc4202022-12-21T20:48:20ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-03-011010.3389/fcimb.2020.00072505380Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo IndividualsRene Arredondo-Hernández0Max Schmulson1Patricia Orduña2Gamaliel López-Leal3Angel-Mario Zarate4Gerardo Alanis-Funes5Luis David Alcaraz6Rubí Santiago-Cruz7Miguel A. Cevallos8Antonio R. Villa9Samuel Ponce-de-León Rosales10Yolanda López-Vidal11Consorcio Mexicano para el Estudio del Microbioma HumanoLaboratorio de Microbioma, División de Estudios de Posgrado y División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Hígado, Páncreas y Motilidad (HIPAM), Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Microbioma, División de Estudios de Posgrado y División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoPrograma de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoHospital General de México, Dr. Eduardo Liceaga, Mexico City, MexicoTecnologico de Monterrey, School of Medicine and Health Sciences, Monterrey, MexicoDepartamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, MexicoPrograma de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoCentro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, MexicoLaboratorio de Microbioma, División de Estudios de Posgrado y División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Microbioma, División de Estudios de Posgrado y División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoPrograma de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoIrritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunction with colonic mucosa dysbiosis, is necessary to determine IBS physiopathology and the effects of environmental pressures. Using a simple polygenic model, host single nucleotide polymorphisms (SNPs) and the taxonomic compositions of microbiota were compared between IBS patients and healthy subjects. As proof of concept, five IBS-Rome III patients and five healthy controls (HCs) were systematically studied. The human and bacterial intestinal metagenome of each subject was taxonomically annotated and screened for previously annotated IBS, ulcerative colitis, and Crohn's disease-associated SNPs or taxon abundance. Dietary data and fecal markers were collected and associated with the intestinal microbiome. However, more than 1,000 variants were found, and at least 76 SNPs differentiated IBS patients from HCs, as did associations with 4 phyla and 10 bacterial genera. In this study, we found elements supporting a polygenic background, with frequent variants, among the Mestizo population, and the colonic mucosal enrichment of Bacteroides, Alteromonas, Neisseria, Streptococcus, and Microbacterium, may serve as a hallmark for IBS.https://www.frontiersin.org/article/10.3389/fcimb.2020.00072/fullIBSSNPsmicrobiotaBacteroidespolygenic SNPs |
| spellingShingle | Rene Arredondo-Hernández Max Schmulson Patricia Orduña Gamaliel López-Leal Angel-Mario Zarate Gerardo Alanis-Funes Luis David Alcaraz Rubí Santiago-Cruz Miguel A. Cevallos Antonio R. Villa Samuel Ponce-de-León Rosales Yolanda López-Vidal Consorcio Mexicano para el Estudio del Microbioma Humano Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals Frontiers in Cellular and Infection Microbiology IBS SNPs microbiota Bacteroides polygenic SNPs |
| title | Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals |
| title_full | Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals |
| title_fullStr | Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals |
| title_full_unstemmed | Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals |
| title_short | Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals |
| title_sort | mucosal microbiome profiles polygenic irritable bowel syndrome in mestizo individuals |
| topic | IBS SNPs microbiota Bacteroides polygenic SNPs |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00072/full |
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