Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses
Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delive...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Drug Delivery |
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| Online Access: | http://dx.doi.org/10.1080/10717544.2018.1450909 |
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| author | Weiping Cao Margarita Mishina Samuel Amoah Wadzanai P. Mboko Caitlin Bohannon James McCoy Suresh K. Mittal Shivaprakash Gangappa Suryaprakash Sambhara |
| author_facet | Weiping Cao Margarita Mishina Samuel Amoah Wadzanai P. Mboko Caitlin Bohannon James McCoy Suresh K. Mittal Shivaprakash Gangappa Suryaprakash Sambhara |
| author_sort | Weiping Cao |
| collection | DOAJ |
| description | Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet™ or in vivo-jetPEI®, and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed TH1/TH2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines. |
| first_indexed | 2024-12-10T09:19:55Z |
| format | Article |
| id | doaj.art-bd29deac68df40f4a89554a8bf69ab77 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-10T09:19:55Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-bd29deac68df40f4a89554a8bf69ab772022-12-22T01:54:44ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642018-01-0125177377910.1080/10717544.2018.14509091450909Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responsesWeiping Cao0Margarita Mishina1Samuel Amoah2Wadzanai P. Mboko3Caitlin Bohannon4James McCoy5Suresh K. Mittal6Shivaprakash Gangappa7Suryaprakash Sambhara8National Center for Immunization and Respiratory DiseasesNational Center for Immunization and Respiratory DiseasesNational Center for Immunization and Respiratory DiseasesPurdue UniversityNational Center for Immunization and Respiratory DiseasesEmory UniversityPurdue UniversityNational Center for Immunization and Respiratory DiseasesNational Center for Immunization and Respiratory DiseasesAvian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet™ or in vivo-jetPEI®, and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed TH1/TH2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines.http://dx.doi.org/10.1080/10717544.2018.1450909cationic polymersh5n1 vaccineadaptive immunityprotective immunitymouse modelinfluenza |
| spellingShingle | Weiping Cao Margarita Mishina Samuel Amoah Wadzanai P. Mboko Caitlin Bohannon James McCoy Suresh K. Mittal Shivaprakash Gangappa Suryaprakash Sambhara Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses Drug Delivery cationic polymers h5n1 vaccine adaptive immunity protective immunity mouse model influenza |
| title | Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses |
| title_full | Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses |
| title_fullStr | Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses |
| title_full_unstemmed | Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses |
| title_short | Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses |
| title_sort | nasal delivery of h5n1 avian influenza vaccine formulated with genjet™ or in vivo jetpei r induces enhanced serological cellular and protective immune responses |
| topic | cationic polymers h5n1 vaccine adaptive immunity protective immunity mouse model influenza |
| url | http://dx.doi.org/10.1080/10717544.2018.1450909 |
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