<b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010
The nutritional requirements of A. brasiliensis in culture media were assessed by supplementing a basal medium (g L-1): (glucose, 10, KH2PO4, 1, MgSO4.7H2O, 0.5, [NH4]2SO4, 1, pH 5.5) with CaCl2, trace elements (FeSO4.7H2O; MnCl2.4H2O; ZnSO4.7H2O; CuSO4.5H2O), casein, yeast extract, peptone, B-vitam...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2011-02-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7010 |
| _version_ | 1811326281361391616 |
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| author | Pascoal José Gaspar Júnior Márcia Mayumi Tomizawa Rosane Freitas Schwan Danny Lee Rinker Eustáquio Souza Dias |
| author_facet | Pascoal José Gaspar Júnior Márcia Mayumi Tomizawa Rosane Freitas Schwan Danny Lee Rinker Eustáquio Souza Dias |
| author_sort | Pascoal José Gaspar Júnior |
| collection | DOAJ |
| description | The nutritional requirements of A. brasiliensis in culture media were assessed by supplementing a basal medium (g L-1): (glucose, 10, KH2PO4, 1, MgSO4.7H2O, 0.5, [NH4]2SO4, 1, pH 5.5) with CaCl2, trace elements (FeSO4.7H2O; MnCl2.4H2O; ZnSO4.7H2O; CuSO4.5H2O), casein, yeast extract, peptone, B-vitamins or amino acids. Evaluations were based on the mycelial growth in solid or liquid culture (mm day-1 or mg day-1) and visual analysis of the colony. The addition of CaCl2 and trace elements was very important for the major mycelial growth of the fungi. The addition of casein and inositol to the medium did not have a significant effect on growth. The best growth result in solid medium was obtained with the basal medium plus the addition of yeast extract and peptone. In relation to the other nutrient sources, the mycelial growth in the presence of amino acids darkened the medium after two weeks. The addition of B-vitamins to the basal medium lead to slower mycelial growth; however, growth was more visually dense when compared to other nutritional sources. B-vitamins added separately did not have the same result, suggesting that the fungus requires two or more vitamins at the same time for better mycelial growth. |
| first_indexed | 2024-04-13T14:47:14Z |
| format | Article |
| id | doaj.art-bd2a08a13f95484d8bcb07d13d824f76 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T14:47:14Z |
| publishDate | 2011-02-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-bd2a08a13f95484d8bcb07d13d824f762022-12-22T02:42:42ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2011-02-0133110.4025/actascibiolsci.v33i1.7010<b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010Pascoal José Gaspar Júnior0Márcia Mayumi Tomizawa1Rosane Freitas Schwan2Danny Lee Rinker3Eustáquio Souza Dias4Centro Universitário de Formiga - UNIFOR-MGUniversidade Federal de LavrasUniversidade Federal de LavrasUniversity of GuelphUniversidade Federal de Lavras, Departamento de Biologia, Setor de Microbiologia.The nutritional requirements of A. brasiliensis in culture media were assessed by supplementing a basal medium (g L-1): (glucose, 10, KH2PO4, 1, MgSO4.7H2O, 0.5, [NH4]2SO4, 1, pH 5.5) with CaCl2, trace elements (FeSO4.7H2O; MnCl2.4H2O; ZnSO4.7H2O; CuSO4.5H2O), casein, yeast extract, peptone, B-vitamins or amino acids. Evaluations were based on the mycelial growth in solid or liquid culture (mm day-1 or mg day-1) and visual analysis of the colony. The addition of CaCl2 and trace elements was very important for the major mycelial growth of the fungi. The addition of casein and inositol to the medium did not have a significant effect on growth. The best growth result in solid medium was obtained with the basal medium plus the addition of yeast extract and peptone. In relation to the other nutrient sources, the mycelial growth in the presence of amino acids darkened the medium after two weeks. The addition of B-vitamins to the basal medium lead to slower mycelial growth; however, growth was more visually dense when compared to other nutritional sources. B-vitamins added separately did not have the same result, suggesting that the fungus requires two or more vitamins at the same time for better mycelial growth.https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7010Mushroomtrace elementsB-vitaminsamino acids |
| spellingShingle | Pascoal José Gaspar Júnior Márcia Mayumi Tomizawa Rosane Freitas Schwan Danny Lee Rinker Eustáquio Souza Dias <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 Acta Scientiarum: Biological Sciences Mushroom trace elements B-vitamins amino acids |
| title | <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 |
| title_full | <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 |
| title_fullStr | <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 |
| title_full_unstemmed | <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 |
| title_short | <b>Nutritional Requirements for Growth of <em>Agaricus brasiliensis</em></b> - doi: 10.4025/actascibiolsci.v33i1.7010 |
| title_sort | b nutritional requirements for growth of em agaricus brasiliensis em b doi 10 4025 actascibiolsci v33i1 7010 |
| topic | Mushroom trace elements B-vitamins amino acids |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7010 |
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