Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol
These data are related to the research article entitled “Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion”. In this article, the peptide and free amino acid profile of egg white gastrointestinal in vitro digests is shown. E...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235234092030826X |
| _version_ | 1819007170134409216 |
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| author | Marta Santos-Hernández Beatriz Miralles Lourdes Amigo Isidra Recio |
| author_facet | Marta Santos-Hernández Beatriz Miralles Lourdes Amigo Isidra Recio |
| author_sort | Marta Santos-Hernández |
| collection | DOAJ |
| description | These data are related to the research article entitled “Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion”. In this article, the peptide and free amino acid profile of egg white gastrointestinal in vitro digests is shown. Egg white proteins were digested following the INFOGEST gastrointestinal digestion protocol. Different time points of gastric and intestinal digestion were characterized regarding protein, peptide and amino acid content. Protein degradation was followed by SDS-PAGE where some electrophoretic bands were identified by MALDI-TOF/TOF after tryptic digestion. Moreover, the molecular weight distribution of egg white peptides found at different times of gastrointestinal digestion was performed using MALDI-TOF. Peptides identified from the most abundant egg white proteins by tandem mass spectrometry were represented using a peptide profile tool and raw data are given in table format. These results reveal the protein regions resistant to digestion and illustrate the free amino acid profile of egg white protein at the end of the digestion process. These data can be used for nutritional purposes and to identify allergen epitopes or bioactive sequences. |
| first_indexed | 2024-12-21T00:20:19Z |
| format | Article |
| id | doaj.art-bd2a61513b0c420ca72798f1b20221c1 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-21T00:20:19Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-bd2a61513b0c420ca72798f1b20221c12022-12-21T19:22:06ZengElsevierData in Brief2352-34092020-08-0131105932Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized ProtocolMarta Santos-Hernández0Beatriz Miralles1Lourdes Amigo2Isidra Recio3Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, SpainCorresponding author.; Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, SpainThese data are related to the research article entitled “Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion”. In this article, the peptide and free amino acid profile of egg white gastrointestinal in vitro digests is shown. Egg white proteins were digested following the INFOGEST gastrointestinal digestion protocol. Different time points of gastric and intestinal digestion were characterized regarding protein, peptide and amino acid content. Protein degradation was followed by SDS-PAGE where some electrophoretic bands were identified by MALDI-TOF/TOF after tryptic digestion. Moreover, the molecular weight distribution of egg white peptides found at different times of gastrointestinal digestion was performed using MALDI-TOF. Peptides identified from the most abundant egg white proteins by tandem mass spectrometry were represented using a peptide profile tool and raw data are given in table format. These results reveal the protein regions resistant to digestion and illustrate the free amino acid profile of egg white protein at the end of the digestion process. These data can be used for nutritional purposes and to identify allergen epitopes or bioactive sequences.http://www.sciencedirect.com/science/article/pii/S235234092030826XEgg white proteinCharacterizationMass spectrometryMaldi-tofPeptidomics |
| spellingShingle | Marta Santos-Hernández Beatriz Miralles Lourdes Amigo Isidra Recio Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol Data in Brief Egg white protein Characterization Mass spectrometry Maldi-tof Peptidomics |
| title | Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol |
| title_full | Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol |
| title_fullStr | Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol |
| title_full_unstemmed | Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol |
| title_short | Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol |
| title_sort | peptidomic data of egg white gastrointestinal digests prepared using the infogest harmonized protocol |
| topic | Egg white protein Characterization Mass spectrometry Maldi-tof Peptidomics |
| url | http://www.sciencedirect.com/science/article/pii/S235234092030826X |
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