Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study
Objective: To explore the regulatory effect of recombinant amelogenin peptide TRAP on the remineralization of early enamel carious lesions.Methods: Forty-eight bovine enamel blocks that prepared initial lesions in vitro were split at random into four groups for immersion treatment for 12 days: 1) re...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1076265/full |
| _version_ | 1797945491448659968 |
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| author | Yaru Li Yaru Li Yiwei Li Yiwei Li Qinghua Bai Qinghua Bai Mingzhu Wen Mingzhu Wen Dandan Ma Yisha Lin Yisha Lin Jinpu Chu |
| author_facet | Yaru Li Yaru Li Yiwei Li Yiwei Li Qinghua Bai Qinghua Bai Mingzhu Wen Mingzhu Wen Dandan Ma Yisha Lin Yisha Lin Jinpu Chu |
| author_sort | Yaru Li |
| collection | DOAJ |
| description | Objective: To explore the regulatory effect of recombinant amelogenin peptide TRAP on the remineralization of early enamel carious lesions.Methods: Forty-eight bovine enamel blocks that prepared initial lesions in vitro were split at random into four groups for immersion treatment for 12 days: 1) remineralizing medium; 2) studied peptide 1 (consisting of the N- and C-termini of porcine amelogenin) + remineralizing medium; 3) studied peptide 2 (TRAP) + remineralizing medium; 4) fluoride + remineralizing medium. After demineralization and remineralization immersion, each specimen’s mean mineral loss and lesion depth were measured using micro-computed tomography (micro-CT). The changes in lesion depth (∆LD) and mineral gain (∆Z) were computed following remineralization. The enamel samples were then cut into sections and examined with polarized light microscopy (PLM). The cross-section morphology was observed by scanning electron microscopy (SEM). The crystal phase was analyzed by an X-ray micro-diffractometer (XRD). The calcium-binding properties were determined using isothermal titration calorimetry (ITC).Results: Micro-CT analysis revealed a significant reduction in mineral loss in the four groups following the remineralization treatment (p < 0.05). The treatment with fluoride resulted in the greatest ∆Z and ∆LD, whereas the treatment with a remineralizing medium showed the least ∆Z and ∆LD among all groups. The ∆Z and ∆LD of the studied peptide 1 and studied peptide 2 groups were greater than those of the remineralizing medium group. However, there was no significant difference between the studied peptide 1 and studied peptide 2 groups (p > 0.05). All of the samples that the PLM analyzed had a thickening of the surface layer. A negative birefringent band changed in the lesion’s body. The SEM displayed that minerals were formed in all four groups of samples. The XRD results indicated that the products of remineralization of the studied peptide were hydroxyapatite crystals (HA). ITC showed that there were two binding modes between the calcium and peptide TRAP.Conclusion: This study confirmed the potential of the recombinant amelogenin peptide TRAP as a key functional motif of amelogenin protein for enamel remineralization and provided a promising biomaterial for remineralization in initial enamel carious lesion treatment. |
| first_indexed | 2024-04-10T20:55:57Z |
| format | Article |
| id | doaj.art-bd2b1a70b45743ea97162b0caa93df10 |
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| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-10T20:55:57Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-bd2b1a70b45743ea97162b0caa93df102023-01-23T06:29:20ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-01-011410.3389/fphys.2023.10762651076265Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro studyYaru Li0Yaru Li1Yiwei Li2Yiwei Li3Qinghua Bai4Qinghua Bai5Mingzhu Wen6Mingzhu Wen7Dandan Ma8Yisha Lin9Yisha Lin10Jinpu Chu11The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Stomatology, Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Stomatology, Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Stomatology, Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Stomatology, Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Stomatology, Zhengzhou University, Zhengzhou, ChinaThe First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaObjective: To explore the regulatory effect of recombinant amelogenin peptide TRAP on the remineralization of early enamel carious lesions.Methods: Forty-eight bovine enamel blocks that prepared initial lesions in vitro were split at random into four groups for immersion treatment for 12 days: 1) remineralizing medium; 2) studied peptide 1 (consisting of the N- and C-termini of porcine amelogenin) + remineralizing medium; 3) studied peptide 2 (TRAP) + remineralizing medium; 4) fluoride + remineralizing medium. After demineralization and remineralization immersion, each specimen’s mean mineral loss and lesion depth were measured using micro-computed tomography (micro-CT). The changes in lesion depth (∆LD) and mineral gain (∆Z) were computed following remineralization. The enamel samples were then cut into sections and examined with polarized light microscopy (PLM). The cross-section morphology was observed by scanning electron microscopy (SEM). The crystal phase was analyzed by an X-ray micro-diffractometer (XRD). The calcium-binding properties were determined using isothermal titration calorimetry (ITC).Results: Micro-CT analysis revealed a significant reduction in mineral loss in the four groups following the remineralization treatment (p < 0.05). The treatment with fluoride resulted in the greatest ∆Z and ∆LD, whereas the treatment with a remineralizing medium showed the least ∆Z and ∆LD among all groups. The ∆Z and ∆LD of the studied peptide 1 and studied peptide 2 groups were greater than those of the remineralizing medium group. However, there was no significant difference between the studied peptide 1 and studied peptide 2 groups (p > 0.05). All of the samples that the PLM analyzed had a thickening of the surface layer. A negative birefringent band changed in the lesion’s body. The SEM displayed that minerals were formed in all four groups of samples. The XRD results indicated that the products of remineralization of the studied peptide were hydroxyapatite crystals (HA). ITC showed that there were two binding modes between the calcium and peptide TRAP.Conclusion: This study confirmed the potential of the recombinant amelogenin peptide TRAP as a key functional motif of amelogenin protein for enamel remineralization and provided a promising biomaterial for remineralization in initial enamel carious lesion treatment.https://www.frontiersin.org/articles/10.3389/fphys.2023.1076265/fullpeptide TRAPenamel cariesremineralizationamelogeninmicro-CT |
| spellingShingle | Yaru Li Yaru Li Yiwei Li Yiwei Li Qinghua Bai Qinghua Bai Mingzhu Wen Mingzhu Wen Dandan Ma Yisha Lin Yisha Lin Jinpu Chu Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study Frontiers in Physiology peptide TRAP enamel caries remineralization amelogenin micro-CT |
| title | Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study |
| title_full | Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study |
| title_fullStr | Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study |
| title_full_unstemmed | Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study |
| title_short | Recombinant amelogenin peptide TRAP promoting remineralization of early enamel caries: An in vitro study |
| title_sort | recombinant amelogenin peptide trap promoting remineralization of early enamel caries an in vitro study |
| topic | peptide TRAP enamel caries remineralization amelogenin micro-CT |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1076265/full |
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