Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in O...
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Frontiers Media S.A.
2021-11-01
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Series: | Frontiers in Pediatrics |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2021.732973/full |
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author | Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni |
author_facet | Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni |
author_sort | Shanshan Liu |
collection | DOAJ |
description | Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time. |
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issn | 2296-2360 |
language | English |
last_indexed | 2024-12-14T14:55:28Z |
publishDate | 2021-11-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Pediatrics |
spelling | doaj.art-bd2d2aa0bf6245e380887d4151a277342022-12-21T22:57:00ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602021-11-01910.3389/fped.2021.732973732973Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With EffusionShanshan Liu0Shanshan Liu1Lining Guo2Lining Guo3Min Chen4Min Chen5Wei Liu6Wei Liu7Yanhong Li8Yanhong Li9Xiaoxu Wang10Xiaoxu Wang11Shilan Li12Shilan Li13Jie Zhang14Jie Zhang15Xin Ni16Xin Ni17Department of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaObjectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time.https://www.frontiersin.org/articles/10.3389/fped.2021.732973/fullotitis media with effusioncaspase-1interleukin-1βinterleukin-18inflammasomeschildren |
spellingShingle | Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion Frontiers in Pediatrics otitis media with effusion caspase-1 interleukin-1β interleukin-18 inflammasomes children |
title | Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
title_full | Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
title_fullStr | Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
title_full_unstemmed | Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
title_short | Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
title_sort | evaluation of caspase 1 interleukin 1β and interleukin 18 in the middle ear effusion in children with otitis media with effusion |
topic | otitis media with effusion caspase-1 interleukin-1β interleukin-18 inflammasomes children |
url | https://www.frontiersin.org/articles/10.3389/fped.2021.732973/full |
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