Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion

Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in O...

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Main Authors: Shanshan Liu, Lining Guo, Min Chen, Wei Liu, Yanhong Li, Xiaoxu Wang, Shilan Li, Jie Zhang, Xin Ni
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Pediatrics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fped.2021.732973/full
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author Shanshan Liu
Shanshan Liu
Lining Guo
Lining Guo
Min Chen
Min Chen
Wei Liu
Wei Liu
Yanhong Li
Yanhong Li
Xiaoxu Wang
Xiaoxu Wang
Shilan Li
Shilan Li
Jie Zhang
Jie Zhang
Xin Ni
Xin Ni
author_facet Shanshan Liu
Shanshan Liu
Lining Guo
Lining Guo
Min Chen
Min Chen
Wei Liu
Wei Liu
Yanhong Li
Yanhong Li
Xiaoxu Wang
Xiaoxu Wang
Shilan Li
Shilan Li
Jie Zhang
Jie Zhang
Xin Ni
Xin Ni
author_sort Shanshan Liu
collection DOAJ
description Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time.
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spelling doaj.art-bd2d2aa0bf6245e380887d4151a277342022-12-21T22:57:00ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602021-11-01910.3389/fped.2021.732973732973Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With EffusionShanshan Liu0Shanshan Liu1Lining Guo2Lining Guo3Min Chen4Min Chen5Wei Liu6Wei Liu7Yanhong Li8Yanhong Li9Xiaoxu Wang10Xiaoxu Wang11Shilan Li12Shilan Li13Jie Zhang14Jie Zhang15Xin Ni16Xin Ni17Department of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, ChinaBeijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck Surgery, Beijing, ChinaObjectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time.https://www.frontiersin.org/articles/10.3389/fped.2021.732973/fullotitis media with effusioncaspase-1interleukin-1βinterleukin-18inflammasomeschildren
spellingShingle Shanshan Liu
Shanshan Liu
Lining Guo
Lining Guo
Min Chen
Min Chen
Wei Liu
Wei Liu
Yanhong Li
Yanhong Li
Xiaoxu Wang
Xiaoxu Wang
Shilan Li
Shilan Li
Jie Zhang
Jie Zhang
Xin Ni
Xin Ni
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
Frontiers in Pediatrics
otitis media with effusion
caspase-1
interleukin-1β
interleukin-18
inflammasomes
children
title Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
title_full Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
title_fullStr Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
title_full_unstemmed Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
title_short Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
title_sort evaluation of caspase 1 interleukin 1β and interleukin 18 in the middle ear effusion in children with otitis media with effusion
topic otitis media with effusion
caspase-1
interleukin-1β
interleukin-18
inflammasomes
children
url https://www.frontiersin.org/articles/10.3389/fped.2021.732973/full
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