Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-15540-1 |
| _version_ | 1818717088439599104 |
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| author | Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf |
| author_facet | Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf |
| author_sort | Johan O. L. Andreasson |
| collection | DOAJ |
| description | RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme. |
| first_indexed | 2024-12-17T19:29:36Z |
| format | Article |
| id | doaj.art-bd2de6930e9143e08b42ce2dd005876a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T19:29:36Z |
| publishDate | 2020-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bd2de6930e9143e08b42ce2dd005876a2022-12-21T21:35:17ZengNature PortfolioNature Communications2041-17232020-04-0111111110.1038/s41467-020-15540-1Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozymeJohan O. L. Andreasson0Andrew Savinov1Steven M. Block2William J. Greenleaf3Department of Genetics, Stanford UniversityBiophysics Program, Stanford UniversityDepartment of Biology, Stanford UniversityDepartment of Genetics, Stanford UniversityRNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.https://doi.org/10.1038/s41467-020-15540-1 |
| spellingShingle | Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme Nature Communications |
| title | Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme |
| title_full | Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme |
| title_fullStr | Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme |
| title_full_unstemmed | Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme |
| title_short | Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme |
| title_sort | comprehensive sequence to function mapping of cofactor dependent rna catalysis in the glms ribozyme |
| url | https://doi.org/10.1038/s41467-020-15540-1 |
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