Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats
This study was designed to investigate the effects of aqueous, ethanol and crude extracts of <em>Chromolaena odorata</em> leaf on haemostatic mechanism of wistar rats and its possible <em>in vitro</em> use in coagulation study. Fifty wistar rats of both sexes weighing between...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2016-12-01
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| Series: | Journal of Biological Research |
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| Online Access: | http://www.pagepressjournals.org/index.php/jbr/article/view/6211 |
| _version_ | 1818457279757811712 |
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| author | Henshaw Uchechi Okoroiwu Item Justin Atangwho Emmanuel Kufre Uko Okafor Ifeyinwa Maryann |
| author_facet | Henshaw Uchechi Okoroiwu Item Justin Atangwho Emmanuel Kufre Uko Okafor Ifeyinwa Maryann |
| author_sort | Henshaw Uchechi Okoroiwu |
| collection | DOAJ |
| description | This study was designed to investigate the effects of aqueous, ethanol and crude extracts of <em>Chromolaena odorata</em> leaf on haemostatic mechanism of wistar rats and its possible <em>in vitro</em> use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke’s and Ivy’s methods, respectively. The prothrombin time was performed using the Quick’s one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed <em>in vitro</em> activity. The study showed the <em>in vivo</em> haemostatic properties of <em>Chromolaena odorata</em> leaf extracts and its possible use in <em>in vitro</em> coagulation study. |
| first_indexed | 2024-12-14T22:40:03Z |
| format | Article |
| id | doaj.art-bd2ec94fc2094ffd8632b63d09fc28f4 |
| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-12-14T22:40:03Z |
| publishDate | 2016-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-bd2ec94fc2094ffd8632b63d09fc28f42022-12-21T22:45:01ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302016-12-0189210.4081/jbr.2016.62115057Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar ratsHenshaw Uchechi Okoroiwu0Item Justin Atangwho1Emmanuel Kufre Uko2Okafor Ifeyinwa Maryann3Department of Medical Laboratory Science, University of Calabar, CalabarDepartment of Biochemistry, University of Calabar, CalabarDepartment of Medical Laboratory Science, University of Calabar, CalabarDepartment of Medical Laboratory Science, University of Calabar, CalabarThis study was designed to investigate the effects of aqueous, ethanol and crude extracts of <em>Chromolaena odorata</em> leaf on haemostatic mechanism of wistar rats and its possible <em>in vitro</em> use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke’s and Ivy’s methods, respectively. The prothrombin time was performed using the Quick’s one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed <em>in vitro</em> activity. The study showed the <em>in vivo</em> haemostatic properties of <em>Chromolaena odorata</em> leaf extracts and its possible use in <em>in vitro</em> coagulation study.http://www.pagepressjournals.org/index.php/jbr/article/view/6211Chromolaena odorataHaemostatic activityHaemostasis |
| spellingShingle | Henshaw Uchechi Okoroiwu Item Justin Atangwho Emmanuel Kufre Uko Okafor Ifeyinwa Maryann Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats Journal of Biological Research Chromolaena odorata Haemostatic activity Haemostasis |
| title | Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats |
| title_full | Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats |
| title_fullStr | Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats |
| title_full_unstemmed | Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats |
| title_short | Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats |
| title_sort | haemostatic property of chromolaena odorata leaf extracts in vitro and in vivo evaluation in wistar rats |
| topic | Chromolaena odorata Haemostatic activity Haemostasis |
| url | http://www.pagepressjournals.org/index.php/jbr/article/view/6211 |
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