Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche
In a previous study, we established a prenatal surgical approach and transplanted mesenchymal stem cells (MSCs) into the fetal rat spinal column to treat neural tube defects (NTDs). We found that the transplanted MSCs survived and differentiated into neural lineage cells. Various cytokines and extra...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-06-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368915X689910 |
| _version_ | 1818939175969226752 |
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| author | Songying Cao Xiaowei Wei Hui Li Jianing Miao Guifeng Zhao Di Wu Bo Liu Yi Zhang Hui Gu Lili Wang Yang Fan Dong An Zhengwei Yuan |
| author_facet | Songying Cao Xiaowei Wei Hui Li Jianing Miao Guifeng Zhao Di Wu Bo Liu Yi Zhang Hui Gu Lili Wang Yang Fan Dong An Zhengwei Yuan |
| author_sort | Songying Cao |
| collection | DOAJ |
| description | In a previous study, we established a prenatal surgical approach and transplanted mesenchymal stem cells (MSCs) into the fetal rat spinal column to treat neural tube defects (NTDs). We found that the transplanted MSCs survived and differentiated into neural lineage cells. Various cytokines and extracellular signaling systems in the spinal cord niche play an important role in cell differentiation. In this study, we observed the differentiation of transplanted MSCs in different spinal cord niches and further observed the expression of neurotrophic factors and growth factors in the spinal cord at different developmental stages to explore the mechanism of MSC differentiation in different spinal cord niches. The results showed that transplanted MSCs expressed markers of neural precursor cells (nestin), neurogliocytes (GFAP), and neurons (β-tubulin). The percentages of GFP + /nestin + double-positive cells in transplanted MSCs in E16, P1, and P21 rats were 18.31%, 12.18%, and 5.06%, respectively. The percentages of GFP + /GFAP + double-positive cells in E16, P1, and P21 rats were 32.01%, 15.35%, and 12.56%, respectively. The percentages of GFP + /β-tubulin + double-positive cells in E16, P1, and P21 were 11.76%, 7.62%, and 4.88%, respectively. The differentiation rates of MSCs in embryonic spinal cords were significantly higher than in postnatal spinal cords ( p < 0.05). We found that the transplanted MSCs expressed synapsin-1 at different developmental stages. After MSC transplantation, we observed that neurotrophic factor-3 (NT-3), fibroblast growth factor-2 (FGF-2), FGF-8, transforming growth factor-α (TGF-α), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) significantly increased in the MSC transplantation group compared with the blank injection group. Furthermore, FGF-2 and VEGF expression were positively correlated with the number of surviving MSCs. In addition, we found that the expression of brain-derived neurotrophic factor (BDNF), NT-3, FGF-8, TGF-β, epidermal growth factor (EGF), and insulin-like growth factor (IGF) decreased with age, and the expression of FGF-2, FGF-10, FGF-20, TGF-α, and PDGF increased with age. Our data suggest that the embryonic spinal cord niche is more conducive to MSC differentiation after transplantation. |
| first_indexed | 2024-12-20T06:19:35Z |
| format | Article |
| id | doaj.art-bd30e4e462f94f048a5d83c089c2ef19 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-20T06:19:35Z |
| publishDate | 2016-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-bd30e4e462f94f048a5d83c089c2ef192022-12-21T19:50:27ZengSAGE PublishingCell Transplantation0963-68971555-38922016-06-012510.3727/096368915X689910Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord NicheSongying Cao0Xiaowei Wei1Hui Li2Jianing Miao3Guifeng Zhao4Di Wu5Bo Liu6Yi Zhang7Hui Gu8Lili Wang9Yang Fan10Dong An11Zhengwei Yuan12Department of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Pediatric Surgery, Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital, China Medical University, Shenyang, ChinaIn a previous study, we established a prenatal surgical approach and transplanted mesenchymal stem cells (MSCs) into the fetal rat spinal column to treat neural tube defects (NTDs). We found that the transplanted MSCs survived and differentiated into neural lineage cells. Various cytokines and extracellular signaling systems in the spinal cord niche play an important role in cell differentiation. In this study, we observed the differentiation of transplanted MSCs in different spinal cord niches and further observed the expression of neurotrophic factors and growth factors in the spinal cord at different developmental stages to explore the mechanism of MSC differentiation in different spinal cord niches. The results showed that transplanted MSCs expressed markers of neural precursor cells (nestin), neurogliocytes (GFAP), and neurons (β-tubulin). The percentages of GFP + /nestin + double-positive cells in transplanted MSCs in E16, P1, and P21 rats were 18.31%, 12.18%, and 5.06%, respectively. The percentages of GFP + /GFAP + double-positive cells in E16, P1, and P21 rats were 32.01%, 15.35%, and 12.56%, respectively. The percentages of GFP + /β-tubulin + double-positive cells in E16, P1, and P21 were 11.76%, 7.62%, and 4.88%, respectively. The differentiation rates of MSCs in embryonic spinal cords were significantly higher than in postnatal spinal cords ( p < 0.05). We found that the transplanted MSCs expressed synapsin-1 at different developmental stages. After MSC transplantation, we observed that neurotrophic factor-3 (NT-3), fibroblast growth factor-2 (FGF-2), FGF-8, transforming growth factor-α (TGF-α), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) significantly increased in the MSC transplantation group compared with the blank injection group. Furthermore, FGF-2 and VEGF expression were positively correlated with the number of surviving MSCs. In addition, we found that the expression of brain-derived neurotrophic factor (BDNF), NT-3, FGF-8, TGF-β, epidermal growth factor (EGF), and insulin-like growth factor (IGF) decreased with age, and the expression of FGF-2, FGF-10, FGF-20, TGF-α, and PDGF increased with age. Our data suggest that the embryonic spinal cord niche is more conducive to MSC differentiation after transplantation.https://doi.org/10.3727/096368915X689910 |
| spellingShingle | Songying Cao Xiaowei Wei Hui Li Jianing Miao Guifeng Zhao Di Wu Bo Liu Yi Zhang Hui Gu Lili Wang Yang Fan Dong An Zhengwei Yuan Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche Cell Transplantation |
| title | Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche |
| title_full | Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche |
| title_fullStr | Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche |
| title_full_unstemmed | Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche |
| title_short | Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche |
| title_sort | comparative study on the differentiation of mesenchymal stem cells between fetal and postnatal rat spinal cord niche |
| url | https://doi.org/10.3727/096368915X689910 |
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