Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels
New technologies and materials could help in this fight against healthcare-associated infections. As the majority of these infections are caused by antibiotic-resistant bacteria, the development of materials with intrinsic antibacterial properties is a promising field of research. We combined chitos...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/8/428 |
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| author | Antonio Laganà Alessio Facciolà Daniela Iannazzo Consuelo Celesti Evelina Polimeni Carmelo Biondo Angela Di Pietro Giuseppa Visalli |
| author_facet | Antonio Laganà Alessio Facciolà Daniela Iannazzo Consuelo Celesti Evelina Polimeni Carmelo Biondo Angela Di Pietro Giuseppa Visalli |
| author_sort | Antonio Laganà |
| collection | DOAJ |
| description | New technologies and materials could help in this fight against healthcare-associated infections. As the majority of these infections are caused by antibiotic-resistant bacteria, the development of materials with intrinsic antibacterial properties is a promising field of research. We combined chitosan (CS), with antibacterial properties, with polyhedral oligomeric silsesquioxanes (POSS), a biocompatible polymer with physico-chemical, mechanical, and rheological properties, creating a hydrogel using cross-linking agent genipin. The antibacterial properties of CS and CS-POSS hydrogels were investigated against nosocomial Gram-positive and Gram-negative bacteria both in terms of membrane damage and surface charge variations, and finally, the anti-biofilm property was studied through confocal microscopy. Both materials showed a good antibacterial capacity against all analyzed strains, both in suspension, with % decreases between 36.36 and 73.58 for CS and 29.86 and 66.04 for CS-POSS, and in plates with % decreases between 55.29 and 78.32 and 17.00 and 53.99 for CS and CS-POSS, respectively. The treated strains compared to the baseline condition showed an important membrane damage, which also determined a variation of surface charges, and finally, for both hydrogels, a remarkable anti-biofilm property was highlighted. Our findings showed a possible future use of these biocompatible materials in the manufacture of medical and surgical devices with intrinsic antibacterial and anti-biofilm properties. |
| first_indexed | 2024-03-10T23:50:49Z |
| format | Article |
| id | doaj.art-bd35ab8734b84e5cb47a985b00041435 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T23:50:49Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-bd35ab8734b84e5cb47a985b000414352023-11-19T01:43:22ZengMDPI AGJournal of Functional Biomaterials2079-49832023-08-0114842810.3390/jfb14080428Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid HydrogelsAntonio Laganà0Alessio Facciolà1Daniela Iannazzo2Consuelo Celesti3Evelina Polimeni4Carmelo Biondo5Angela Di Pietro6Giuseppa Visalli7Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, ItalyDepartment of Electronic Engineering, Industrial Chemistry and Engineering, University of Messina, 98166 Messina, ItalyDepartment of Electronic Engineering, Industrial Chemistry and Engineering, University of Messina, 98166 Messina, ItalyDepartment of Human Pathology, University of Messina, 98125 Messina, ItalyDepartment of Human Pathology, University of Messina, 98125 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98125 Messina, ItalyNew technologies and materials could help in this fight against healthcare-associated infections. As the majority of these infections are caused by antibiotic-resistant bacteria, the development of materials with intrinsic antibacterial properties is a promising field of research. We combined chitosan (CS), with antibacterial properties, with polyhedral oligomeric silsesquioxanes (POSS), a biocompatible polymer with physico-chemical, mechanical, and rheological properties, creating a hydrogel using cross-linking agent genipin. The antibacterial properties of CS and CS-POSS hydrogels were investigated against nosocomial Gram-positive and Gram-negative bacteria both in terms of membrane damage and surface charge variations, and finally, the anti-biofilm property was studied through confocal microscopy. Both materials showed a good antibacterial capacity against all analyzed strains, both in suspension, with % decreases between 36.36 and 73.58 for CS and 29.86 and 66.04 for CS-POSS, and in plates with % decreases between 55.29 and 78.32 and 17.00 and 53.99 for CS and CS-POSS, respectively. The treated strains compared to the baseline condition showed an important membrane damage, which also determined a variation of surface charges, and finally, for both hydrogels, a remarkable anti-biofilm property was highlighted. Our findings showed a possible future use of these biocompatible materials in the manufacture of medical and surgical devices with intrinsic antibacterial and anti-biofilm properties.https://www.mdpi.com/2079-4983/14/8/428healthcare-associated infectionschitosanchitosan-POSShydrogelantibacterial activity |
| spellingShingle | Antonio Laganà Alessio Facciolà Daniela Iannazzo Consuelo Celesti Evelina Polimeni Carmelo Biondo Angela Di Pietro Giuseppa Visalli Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels Journal of Functional Biomaterials healthcare-associated infections chitosan chitosan-POSS hydrogel antibacterial activity |
| title | Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels |
| title_full | Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels |
| title_fullStr | Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels |
| title_full_unstemmed | Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels |
| title_short | Promising Materials in the Fight against Healthcare-Associated Infections: Antibacterial Properties of Chitosan-Polyhedral Oligomeric Silsesquioxanes Hybrid Hydrogels |
| title_sort | promising materials in the fight against healthcare associated infections antibacterial properties of chitosan polyhedral oligomeric silsesquioxanes hybrid hydrogels |
| topic | healthcare-associated infections chitosan chitosan-POSS hydrogel antibacterial activity |
| url | https://www.mdpi.com/2079-4983/14/8/428 |
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