Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status.
Among livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4556373?pdf=render |
| _version_ | 1818021774436073472 |
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| author | Robert Staroń Rachel P L Van Swelm Paweł Lipiński Anna Gajowiak Małgorzata Lenartowicz Aleksandra Bednarz Małgorzata Gajewska Marek Pieszka Coby M M Laarakkers Dorine W Swinkels Rafał R Starzyński |
| author_facet | Robert Staroń Rachel P L Van Swelm Paweł Lipiński Anna Gajowiak Małgorzata Lenartowicz Aleksandra Bednarz Małgorzata Gajewska Marek Pieszka Coby M M Laarakkers Dorine W Swinkels Rafał R Starzyński |
| author_sort | Robert Staroń |
| collection | DOAJ |
| description | Among livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. Moreover, supplementation of essentially all commercially reared piglets with iron entails a need for monitoring the efficacy of this routine practice followed in the swine industry for several decades. Since the discovery of hepcidin many studies confirmed its role as key regulator of iron metabolism and pointed out the assessment of its concentrations in biological fluids as diagnostic tool for iron-related disorder. Here we demonstrate that urine hepcidin-25 levels measured by a combination of weak cation exchange chromatography and time-of-flight mass spectrometry (WCX-TOF MS) are highly correlated with mRNA hepcidin expression in the liver and plasma hepcidin-25 concentrations in anemic and iron-supplemented 28-day old piglets. We also found a high correlation between urine hepcidin level and hepatic non-heme iron content. Our results show that similarly to previously described transgenic mouse models of iron disorders, young pigs constitute a convenient animal model to explore accuracy and relationship between indicators for assessing systemic iron status. |
| first_indexed | 2024-04-14T08:22:28Z |
| format | Article |
| id | doaj.art-bd381ffd2c054f3ea3e406d8a06cecde |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:22:28Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bd381ffd2c054f3ea3e406d8a06cecde2022-12-22T02:04:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013669510.1371/journal.pone.0136695Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status.Robert StarońRachel P L Van SwelmPaweł LipińskiAnna GajowiakMałgorzata LenartowiczAleksandra BednarzMałgorzata GajewskaMarek PieszkaCoby M M LaarakkersDorine W SwinkelsRafał R StarzyńskiAmong livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. Moreover, supplementation of essentially all commercially reared piglets with iron entails a need for monitoring the efficacy of this routine practice followed in the swine industry for several decades. Since the discovery of hepcidin many studies confirmed its role as key regulator of iron metabolism and pointed out the assessment of its concentrations in biological fluids as diagnostic tool for iron-related disorder. Here we demonstrate that urine hepcidin-25 levels measured by a combination of weak cation exchange chromatography and time-of-flight mass spectrometry (WCX-TOF MS) are highly correlated with mRNA hepcidin expression in the liver and plasma hepcidin-25 concentrations in anemic and iron-supplemented 28-day old piglets. We also found a high correlation between urine hepcidin level and hepatic non-heme iron content. Our results show that similarly to previously described transgenic mouse models of iron disorders, young pigs constitute a convenient animal model to explore accuracy and relationship between indicators for assessing systemic iron status.http://europepmc.org/articles/PMC4556373?pdf=render |
| spellingShingle | Robert Staroń Rachel P L Van Swelm Paweł Lipiński Anna Gajowiak Małgorzata Lenartowicz Aleksandra Bednarz Małgorzata Gajewska Marek Pieszka Coby M M Laarakkers Dorine W Swinkels Rafał R Starzyński Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. PLoS ONE |
| title | Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. |
| title_full | Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. |
| title_fullStr | Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. |
| title_full_unstemmed | Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. |
| title_short | Urinary Hepcidin Levels in Iron-Deficient and Iron-Supplemented Piglets Correlate with Hepcidin Hepatic mRNA and Serum Levels and with Body Iron Status. |
| title_sort | urinary hepcidin levels in iron deficient and iron supplemented piglets correlate with hepcidin hepatic mrna and serum levels and with body iron status |
| url | http://europepmc.org/articles/PMC4556373?pdf=render |
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