Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine
For the detection and identification of Candidatus Phytoplasma vitis Flavescence doree - the yellowing grape quarantine pest for the territory of Russia and Candidatus Phytoplasma solani Bois noir - pathogen of blackening crust grape was used one of the existing methods of diagnosis PCR in “real tim...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Peoples’ Friendship University of Russia (RUDN University)
2015-12-01
|
| Series: | RUDN Journal of Agronomy and Animal Industries |
| Subjects: | |
| Online Access: | http://journals.rudn.ru/agronomy/article/view/1505 |
| _version_ | 1819101907154632704 |
|---|---|
| author | G N Matyashova V G Zaets |
| author_facet | G N Matyashova V G Zaets |
| author_sort | G N Matyashova |
| collection | DOAJ |
| description | For the detection and identification of Candidatus Phytoplasma vitis Flavescence doree - the yellowing grape quarantine pest for the territory of Russia and Candidatus Phytoplasma solani Bois noir - pathogen of blackening crust grape was used one of the existing methods of diagnosis PCR in “real time” (RT-PCR). Matched to the fragment 16SrRNA gene species-specific primer systems were tested for false positive and false negative results for the detection of plant material in the above two phytoplasmas. According to the results of PCR in “real time”, it was determined that the studied pairs of primers allow the identification of target species phytoplasmas. Cross-reactions with other species phytoplasmas were not detected. Primers pairs do not give false positive reactions with gram-positive bacteria and some types of bacterial microflora grapes. The primer pairs and probes FDrt and BNrt can be used to detect and identify phytoplasmas - pathogens FD and BN in the regulated articles, as well as monitoring. RT-PCR method is considered to be relatively simple to implement and does not require time-consuming for the detection results. |
| first_indexed | 2024-12-22T01:26:07Z |
| format | Article |
| id | doaj.art-bd3b6ed2f1f240c3838e1a7dafae4be3 |
| institution | Directory Open Access Journal |
| issn | 2312-797X 2312-7988 |
| language | English |
| last_indexed | 2024-12-22T01:26:07Z |
| publishDate | 2015-12-01 |
| publisher | Peoples’ Friendship University of Russia (RUDN University) |
| record_format | Article |
| series | RUDN Journal of Agronomy and Animal Industries |
| spelling | doaj.art-bd3b6ed2f1f240c3838e1a7dafae4be32022-12-21T18:43:36ZengPeoples’ Friendship University of Russia (RUDN University)RUDN Journal of Agronomy and Animal Industries2312-797X2312-79882015-12-010471410.22363/2312-797X-2015-4-7-141505Research of the Real Time PCR method for detection and identification phytoplasmas on grapevineG N Matyashova0V G Zaets1<p>Российский университет дружбы народов</p><p>Российский университет дружбы народов</p>For the detection and identification of Candidatus Phytoplasma vitis Flavescence doree - the yellowing grape quarantine pest for the territory of Russia and Candidatus Phytoplasma solani Bois noir - pathogen of blackening crust grape was used one of the existing methods of diagnosis PCR in “real time” (RT-PCR). Matched to the fragment 16SrRNA gene species-specific primer systems were tested for false positive and false negative results for the detection of plant material in the above two phytoplasmas. According to the results of PCR in “real time”, it was determined that the studied pairs of primers allow the identification of target species phytoplasmas. Cross-reactions with other species phytoplasmas were not detected. Primers pairs do not give false positive reactions with gram-positive bacteria and some types of bacterial microflora grapes. The primer pairs and probes FDrt and BNrt can be used to detect and identify phytoplasmas - pathogens FD and BN in the regulated articles, as well as monitoring. RT-PCR method is considered to be relatively simple to implement and does not require time-consuming for the detection results.http://journals.rudn.ru/agronomy/article/view/1505диагностикафитоплазмыкарантин растенийПЦР |
| spellingShingle | G N Matyashova V G Zaets Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine RUDN Journal of Agronomy and Animal Industries диагностика фитоплазмы карантин растений ПЦР |
| title | Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine |
| title_full | Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine |
| title_fullStr | Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine |
| title_full_unstemmed | Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine |
| title_short | Research of the Real Time PCR method for detection and identification phytoplasmas on grapevine |
| title_sort | research of the real time pcr method for detection and identification phytoplasmas on grapevine |
| topic | диагностика фитоплазмы карантин растений ПЦР |
| url | http://journals.rudn.ru/agronomy/article/view/1505 |
| work_keys_str_mv | AT gnmatyashova researchoftherealtimepcrmethodfordetectionandidentificationphytoplasmasongrapevine AT vgzaets researchoftherealtimepcrmethodfordetectionandidentificationphytoplasmasongrapevine |