Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter
<i>Mannheimia haemolytica</i> is the main pathogen contributing to pneumonic pasteurellosis in sheep. The aim of this study was to investigate the antimicrobial resistance levels in <i>M. haemolytica</i> isolates from the lungs of slaughtered sheep and to examine the genetic...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/12/1991 |
| _version_ | 1797596485959811072 |
|---|---|
| author | Julio Alvarez Johan M. Calderón Bernal Laura Torre-Fuentes Marta Hernández Chris E. Pinto Jimenez Lucas Domínguez José F. Fernández-Garayzábal Ana I. Vela Dolores Cid |
| author_facet | Julio Alvarez Johan M. Calderón Bernal Laura Torre-Fuentes Marta Hernández Chris E. Pinto Jimenez Lucas Domínguez José F. Fernández-Garayzábal Ana I. Vela Dolores Cid |
| author_sort | Julio Alvarez |
| collection | DOAJ |
| description | <i>Mannheimia haemolytica</i> is the main pathogen contributing to pneumonic pasteurellosis in sheep. The aim of this study was to investigate the antimicrobial resistance levels in <i>M. haemolytica</i> isolates from the lungs of slaughtered sheep and to examine the genetic resistance mechanisms involved. A total of 256 <i>M. haemolytica</i> isolates, 169 from lungs with pneumonic lesions and 87 from lungs without lesions, were analyzed by the disk diffusion method for 12 antimicrobials, and the whole genome of 14 isolates was sequenced to identify antimicrobial resistance determinants. Levels of phenotypic resistance ranged from <2% for 10 antimicrobials (amoxicillin, amoxicillin-clavulanic, ceftiofur, cefquinome, lincomycin/spectinomycin, gentamicin, erythromycin, florfenicol, enrofloxacin, and doxycycline) to 4.3% for tetracycline and 89.1% for tylosin. Six isolates carried <i>tetH</i> genes and four isolates carried, in addition, the <i>strA</i> and <i>sul2</i> genes in putative plasmid sequences. No mutations associated with macrolide resistance were identified in 23 rDNA sequences, suggesting that the <i>M. haemolytica</i> phenotypic results for tylosin should be interpreted with care in the absence of well-established epidemiological and clinical breakpoints. The identification of strains phenotypically resistant to tetracycline and of several resistance genes, some of which were present in plasmids, highlights the need for continuous monitoring of susceptibility patterns in <i>Pasteurellaceae</i> isolates from livestock. |
| first_indexed | 2024-03-11T02:51:02Z |
| format | Article |
| id | doaj.art-bd3cf2abbf6c477c895c1398d348276c |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T02:51:02Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-bd3cf2abbf6c477c895c1398d348276c2023-11-18T08:59:03ZengMDPI AGAnimals2076-26152023-06-011312199110.3390/ani13121991Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at SlaughterJulio Alvarez0Johan M. Calderón Bernal1Laura Torre-Fuentes2Marta Hernández3Chris E. Pinto Jimenez4Lucas Domínguez5José F. Fernández-Garayzábal6Ana I. Vela7Dolores Cid8Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainCentro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, 47071 Valladolid, SpainLondon School of Hygiene and Tropical Medicine, University of London, London WC1E 7HT, UKDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain<i>Mannheimia haemolytica</i> is the main pathogen contributing to pneumonic pasteurellosis in sheep. The aim of this study was to investigate the antimicrobial resistance levels in <i>M. haemolytica</i> isolates from the lungs of slaughtered sheep and to examine the genetic resistance mechanisms involved. A total of 256 <i>M. haemolytica</i> isolates, 169 from lungs with pneumonic lesions and 87 from lungs without lesions, were analyzed by the disk diffusion method for 12 antimicrobials, and the whole genome of 14 isolates was sequenced to identify antimicrobial resistance determinants. Levels of phenotypic resistance ranged from <2% for 10 antimicrobials (amoxicillin, amoxicillin-clavulanic, ceftiofur, cefquinome, lincomycin/spectinomycin, gentamicin, erythromycin, florfenicol, enrofloxacin, and doxycycline) to 4.3% for tetracycline and 89.1% for tylosin. Six isolates carried <i>tetH</i> genes and four isolates carried, in addition, the <i>strA</i> and <i>sul2</i> genes in putative plasmid sequences. No mutations associated with macrolide resistance were identified in 23 rDNA sequences, suggesting that the <i>M. haemolytica</i> phenotypic results for tylosin should be interpreted with care in the absence of well-established epidemiological and clinical breakpoints. The identification of strains phenotypically resistant to tetracycline and of several resistance genes, some of which were present in plasmids, highlights the need for continuous monitoring of susceptibility patterns in <i>Pasteurellaceae</i> isolates from livestock.https://www.mdpi.com/2076-2615/13/12/1991<i>Mannheimia haemolytica</i>antimicrobial resistancemobile genetic elements<i>tetH</i><i>strA</i><i>sul2</i> |
| spellingShingle | Julio Alvarez Johan M. Calderón Bernal Laura Torre-Fuentes Marta Hernández Chris E. Pinto Jimenez Lucas Domínguez José F. Fernández-Garayzábal Ana I. Vela Dolores Cid Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter Animals <i>Mannheimia haemolytica</i> antimicrobial resistance mobile genetic elements <i>tetH</i> <i>strA</i> <i>sul2</i> |
| title | Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter |
| title_full | Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter |
| title_fullStr | Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter |
| title_full_unstemmed | Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter |
| title_short | Antimicrobial Susceptibility and Resistance Mechanisms in <i>Mannheimia haemolytica</i> Isolates from Sheep at Slaughter |
| title_sort | antimicrobial susceptibility and resistance mechanisms in i mannheimia haemolytica i isolates from sheep at slaughter |
| topic | <i>Mannheimia haemolytica</i> antimicrobial resistance mobile genetic elements <i>tetH</i> <i>strA</i> <i>sul2</i> |
| url | https://www.mdpi.com/2076-2615/13/12/1991 |
| work_keys_str_mv | AT julioalvarez antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT johanmcalderonbernal antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT lauratorrefuentes antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT martahernandez antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT chrisepintojimenez antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT lucasdominguez antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT joseffernandezgarayzabal antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT anaivela antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter AT dolorescid antimicrobialsusceptibilityandresistancemechanismsinimannheimiahaemolyticaiisolatesfromsheepatslaughter |