RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation
Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will b...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/8/729 |
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| author | Sharda Yadav Navid Kashaninejad Nam-Trung Nguyen |
| author_facet | Sharda Yadav Navid Kashaninejad Nam-Trung Nguyen |
| author_sort | Sharda Yadav |
| collection | DOAJ |
| description | Liver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients. |
| first_indexed | 2024-03-10T18:09:27Z |
| format | Article |
| id | doaj.art-bd3e1083279249ff8eac2ece4f526535 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T18:09:27Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | Micromachines |
| spelling | doaj.art-bd3e1083279249ff8eac2ece4f5265352023-11-20T08:13:52ZengMDPI AGMicromachines2072-666X2020-07-0111872910.3390/mi11080729RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical StimulationSharda Yadav0Navid Kashaninejad1Nam-Trung Nguyen2Queensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University,170 Kessels Road, Brisbane, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University,170 Kessels Road, Brisbane, QLD 4111, AustraliaQueensland Micro- and Nanotechnology Centre, Nathan Campus, Griffith University,170 Kessels Road, Brisbane, QLD 4111, AustraliaLiver cancer, especially hepatocellular carcinoma (HCC), is an aggressive disease with an extremely high mortality rate. Unfortunately, no promising markers are currently available for the early diagnosis of this disease. Thus, a reliable biomarker reflecting the early behaviour of the tumour will be valuable for diagnosis and treatment. The Ras homologous (Rho) GTPases, which belong to the small guanosine triphosphate (GTP) binding proteins, have been reported to play an important role in mediating liver cancer based on their important function in cytoskeletal reorganisation. These proteins can be either oncogenic or tumour suppressors. They are also associated with the acquirement of malignant features by cancer cells. The overexpression of RhoA and Rac1, members of the Rho GTPases, have been linked with carcinogenesis and the progression of different types of cancer. In the quest of elucidating the role of mechanical stimulation in the mechanobiology of liver cancer cells, this paper evaluates the effect of stretching on the expression levels of RhoA and Rac1 in different types of liver cancers. It is shown that that stretching liver cancer cells significantly increases the expression levels of RhoA and Rac1 in HCC and cholangiocarcinoma cell lines. We hypothesise that this relatively simple and sensitive method could be helpful for screening biological features and provide suitable treatment guidance for liver cancer patients.https://www.mdpi.com/2072-666X/11/8/729liver cancerHCCRho GTPasecell-stretchingmechanobiologyRac1 |
| spellingShingle | Sharda Yadav Navid Kashaninejad Nam-Trung Nguyen RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation Micromachines liver cancer HCC Rho GTPase cell-stretching mechanobiology Rac1 |
| title | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
| title_full | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
| title_fullStr | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
| title_full_unstemmed | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
| title_short | RhoA and Rac1 in Liver Cancer Cells: Induction of Overexpression Using Mechanical Stimulation |
| title_sort | rhoa and rac1 in liver cancer cells induction of overexpression using mechanical stimulation |
| topic | liver cancer HCC Rho GTPase cell-stretching mechanobiology Rac1 |
| url | https://www.mdpi.com/2072-666X/11/8/729 |
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